Estimation of the Biopreservative Obtaining Method Modification for Perennial Grasses Silage Making by Fermentative Activity

Abstract

For a long time, scientific research has been carried out at the VNIIMZ on the basis of the Department of Biotechnology in the field of bioconversion of organic resources into biological means intended for use in agriculture as fertilizers, plant growth stimulants, and bioconservants for silage forage crops. One of the promising basic developments of the department is the patented way of obtaining a biokonservant by peat fermentation with excrement of agricultural animals, including extraction of bioactive substances, which, in fact, constitute the basis of the product being formed. The material of the publication presented contains a comparative analysis of a patented method for producing a biokonservant and a modified method in which new biostimulant and extractant are used. The investigated processes were compared in the dynamics of enzyme activity. The results of the studies showed that the introduction of a new multicomponent biostimulator activated the enzymatic activity in the bioconversion process of the initial substrate. Two bursts of oxidation-reduction reaction activity were recorded at the key stages of the process (the oxidation-reduction ratio of the initial mixture was 1.3 and the fermentation product was 1.23 in the new technology versus 0.14 and 0.06 in the base technology, respectively). In this case, the high activity of invertase in the initial substrate provided an intensification of the process of cleavage of complex carbohydrates to monomers. The change in the extractant had no significant effect on the enzymatic activity. The extraction period was characterized by a decrease in the activity of all the enzymes studied, regardless of the technology used. Additional studies of a complex biostimulator are required to increase and grow lactic acid microflora, as in the finished biokonservant by the new technology, the lactic acid microflora titer is nx107 cfu / ml, which is lower by an order of magnitude than in the finished product according to the basic technology.

The Obtaining Process Optimization a New Biological Preservative for Ensiling of Perennial Grasses

“…Он активно работал уже на первых этапах ферментации (инвертазно-целлюлазное отношение по новой технологии составило 1,4 против 0,8 по базовой технологии). РН биоконсервантов изменялась в зависимости от экстрагента: щелочного (6,5) или кислотного (4,9). Активная реакция среды экстрагента оказывала влияние на общую микробную обсемененность.…”

“…Далее все технологические процессы по новой технологии проходили без изменений до этапа экстракции. На этом этапе по новой технологии в качестве экстрагента был выбран раствор уксусной кислоты (СН3СООН) [3,4]. В ходе эксперимента изучали образцы исходной и ферментируемой массы, продукты ферментации и готовые жидкофазные биоконсерванты общепринятыми методами по ГОСТу:…”