Estimation of the biofilm formation of Escherichia coli K-12 by the cell number

Mutants ofLactobacillus plantarumML11-11 Deficient in Co-Aggregation with Yeast Exhibited Reduced Activities of Mixed-Species Biofilm Formation

“…After inoculation, both the single-and the mixed-culture samples were incubated at 30 C for 24 h. Quantification of biofilm formation was done by the crystal violet staining method. [21][22][23] Results presented here are representative of three independent experiments.…”

“…Biofilm formation and the assay protocol were basically as in our previous study. 21) To assay biofilm formation, LAB and yeast overnight cultures were inoculated into fresh YPD at a dilution rate of 1:100 in the single cultures, while for the mixed cultures, both overnight cultures of LAB and yeast were inoculated into fresh YPD at a dilution rate of 1:200. A 96-well polystyrene micro-titer plate (#92696, TPP AG) was used for biofilm formation.…”

Screening of lactic acid bacteria that can form mixed-species biofilm with Saccharomyces cerevisiae

“…[17][18][19] Observation of biofilms by scanning electron microscopy. Mixed-species biofilms were formed on glass coverslips by culturing at 30°C for 24 h in 20 mL of YPD medium in a Petri dish.…”

Mixed-Species Biofilm Formation by Direct Cell-Cell Contact between Brewing Yeasts and Lactic Acid Bacteria

“…20) To assay biofilm formation, stationary phase LAB or yeast cultures were inoculated into fresh YPD at the dilution rate of 1:100 in monoculture, while for co-culture, stationary phase cultures of the LAB and yeast were inoculated into fresh YPD at a dilution rate of 1:200. A 96-well polystyrene micro-titer plate (#92696, TPP AG, Adolf Kühner, Switzerland) was used for biofilm formation.…”

“…After inoculation, both mono-and co-culture samples were incubated at 30 C for 24 h. Quantification of biofilm formation was done by the conventional titer plate method. 14,20,21) y To whom correspondence should be addressed. Note Ten yeasts and 124 LAB were isolated from samples of Fukuyama pot vinegar fermentation collected on the 5th and 11th brewing day, and mixed-species biofilm formation between the yeasts and LAB was investigated ( Table 1).…”