Remarkable LAB-yeast mixed-species biofilm was formed by lactic acid bacteria (LAB) Lactobacillus plantarum
ML11-11 isolated from Fukuyama pot vinegar and Saccharomyces cerevisiae. This mixed-species biofilm formation
increased in proportion to the YPD medium concentration but decreased in proportion to the MRS medium concentration. The effect of
MRS components on mixed-species biofilm formation was investigated in a YPD medium environment, and it was clarified that beef
extract (one of the MRS medium components) decreased mixed-species biofilm formation. On the other hand, manganese sulfate
(another component in MRS) remarkably increased both LAB single- and LAB-yeast mixed-species biofilm formation. LAB single- and
mixed-species biofilm formation were increased in proportion to the manganese sulfate concentration up to 1 mM and 100 μM,
respectively. The growth of L. plantarum ML11-11 was increased significantly by the addition of 10 μM manganese
sulfate and was resistant to higher concentration of up to 100 mM, but growth of S. cerevisiae was sensitive to
manganese ion above 100 μM. These results suggested that mixed-species biofilm formation could be controlled artificially by
controlling the manganese ion level.
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