Estimation of TGF-b1 genotypes in Egyptian smokers; association with FEV1 in COPD patients

Abstract

IntroductionTGF-β1 is a cytokine with many different effects on cell proliferation, differentiation and inflammation and can protect against the development of COPD. This work aims to study the association between COPD and the TGF-β1 gene genotypes.Material and methodsThe study included 70 males: 25 smokers with COPD, 25 resistant smokers, and 20 normal non-smokers as the control. They were subjected to spirometry pre- and post-bronchodilator (FEV1, FEV1/FVC), estimation of serum level of TGF-β1 gene by PCR and RFLP.ResultsThe percent of Pro-Leu was 28% in the COPD group, 84% in the resistant smokers group and 85% in the control group. There was a highly significant statistical difference in FEV1% of predicted associated with the distribution of TGF-β1 gene genotypes: 56.9 ±8.4% with Pro-Leu genotype and 35.5 ±8.8% with Leu-Leu genotype in COPD patients, 93.2 ±6.2% with Pro-Leu genotype and 86.7 ±0.9% with Leu-Leu genotype in the resistant smokers group.ConclusionsThe Pro-allele genotype is associated with increased production of TGF-β1, which has a protective role against the development of COPD and is important in preserving the decline of FEV1 in COPD patients.

Effects of Two Chinese Herbal Formulae for the Treatment of Moderate to Severe Stable Chronic Obstructive Pulmonary Disease: A Multicenter, Double-Blind, Randomized Controlled Trial

“…These findings supported the hypothesis that the two treatments, especially BY granule, benefited to patients with COPD in clinical practice. Interestingly, TGF-β1 could delay the development of COPD, and help repair damage to the lungs of individuals who are at risk of developing COPD, preserving the decline of FEV 1 [20] , [21] . Recent researches provided the levels of TGF-β1 were different at four stages of COPD with highest in stage IV, and inversely correlated with FEV 1 (% predicted) and FVC (% predicted) [22] , [23] .…”

Association of TNF-α–308G/A, SP-B 1580 C/T, IL-13 –1055 C/T gene polymorphisms and latent adenoviral infection with chronic obstructive pulmonary disease in an Egyptian population

“…In a case control study, the distribution of TGF-β1 genotypes in an Egyptian population was demonstrated; the absence of the Pro-Pro alleles in all cases of the study denoted a high predisposition to COPD in Egyptian smokers. Moreover, the Pro allele of the TGF-β1 gene was expressed more commonly in resistant smokers than in smokers who developed COPD, demonstrating a protective role against COPD and an important effect in preserving further decline in forced expiratory volume in the 1 s (FEV 1 ) [ 5 ].…”