Estimation of shear stress values along endothelial tip cells past the lumen of capillary sprouts

Hu, Nien‐Wen; Rodriguez, Camille D; Rey, Julian; Rozenblum, Maximillian; Courtney, Connor; Balogh, Péter; Sarntinoranont, Malisa; Murfee, Walter L.

doi:10.1016/j.mvr.2022.104360

Cited by 7 publications

(10 citation statements)

References 57 publications

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“…Also, the mesentery provides branched microvascular networks and has been previously used for computational estimation of vessel specific hemodynamics. 22,42,43 Also, the 48-80 angiogenesis stimulation, which has been well characterized by our group and others, 28,44 results in robust angiogenic remolding across the hierarchy of networks. Future studies are also needed to evaluate shear stress distributions for alternative remodeling stimuli.…”

Section: Discussionmentioning

confidence: 68%

“…The rat mesentery was used for this study because it is 20–40 μm thick, and its thinness makes it relatively easy to image with a standard epifluorescent microscope. Also, the mesentery provides branched microvascular networks and has been previously used for computational estimation of vessel specific hemodynamics 22,42,43 . Also, the 48–80 angiogenesis stimulation, which has been well characterized by our group and others, 28,44 results in robust angiogenic remolding across the hierarchy of networks.…”

Section: Discussionmentioning

confidence: 96%

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Estimation of shear stress heterogeneity along capillary segments in angiogenic rat mesenteric microvascular networks

Hu,

Lomel,

Rice

et al. 2023

Microcirculation

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ObjectiveFluid shear stress is thought to be a regulator of endothelial cell behavior during angiogenesis. The link, however, requires an understanding of stress values at the capillary level in angiogenic microvascular networks. Critical questions remain. What are the stresses? Do capillaries experience similar stress magnitudes? Can variations explain vessel‐specific behavior? The objective of this study was to estimate segment‐specific shear stresses in angiogenic networks.MethodsImages of angiogenic networks characterized by increased vascular density were obtained from rat mesenteric tissues stimulated by compound 48/80‐induced mast cell degranulation. Vessels were identified by perfusion of a 40 kDa fixable dextran prior to harvesting and immunolabeling for PECAM. Using a network flow‐based segment model with physiologically relevant parameters, stresses were computed per vessel for regions across multiple networks.ResultsStresses ranged from 0.003 to 2328.1 dyne/cm2 and varied dramatically at the capillary level. For all regions, the maximum segmental shear stresses were for capillary segments. Stresses along proximal capillaries branching from arteriole inlets were increased compared to stresses along capillaries in more distal regions.ConclusionsThe results highlight the variability of shear stresses along angiogenic capillaries and motivate new discussions on how endothelial cells may respond in vivo to segment‐specific microenvironment during angiogenesis.

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Section: Discussionmentioning

confidence: 68%

Section: Discussionmentioning

confidence: 96%

Estimation of shear stress heterogeneity along capillary segments in angiogenic rat mesenteric microvascular networks

Hu,

Lomel,

Rice

et al. 2023

Microcirculation

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“…Another work showed that FSS induces differentiation and vasculogenesis of circulating EPCs (Obi et al, 2012). Recently, Hu and colleagues estimated FSS values of endothelial tip cells in the capillary sprout lumen (Hu et al, 2022). Shear stress controls angiogenic sprouting and offers a potential mechanism for modulating vascular density (Galie et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Hemodynamic force dictates endothelial angiogenesis through MIEN1‐ERK/MAPK‐signaling axis

Cheng,

Shi,

et al. 2024

Journal Cellular Physiology

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It is well‐recognized that blood flow at branches and bends of arteries generates disturbed shear stress, which plays a crucial in driving atherosclerosis. Flow‐generated fluid shear stress (FSS), as one of the key hemodynamic factors, is appreciated for its critical involvement in regulating angiogenesis to facilitate wound healing and tissue repair. Endothelial cells can directly sense FSS but the mechanobiological mechanism by which they decode different patterns of FSS to trigger angiogenesis remains unclear. In the current study, laminar shear stress (LSS, 15 dyn/cm2) was employed to mimic physiological blood flow, while disturbed shear stress (DSS, ranging from 0.5 ± 4 dyn/cm2) was applied to simulate pathological conditions. The aim was to investigate how these distinct types of blood flow regulated endothelial angiogenesis. Initially, we observed that DSS impaired angiogenesis and downregulated endogenous vascular endothelial growth factor B (VEGFB) expression compared to LSS. We further found that the changes in membrane protein, migration and invasion enhancer 1 (MIEN1) play a role in regulating ERK/MAPK signaling, thereby contributing to endothelial angiogenesis in response to FSS. We also showed the involvement of MIEN1‐directed cytoskeleton organization. These findings suggest the significance of shear stress in endothelial angiogenesis, thereby enhancing our understanding of the alterations in angiogenesis that occur during the transition from physiological to pathological blood flow.

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“…A more recent microfluidic study demonstrated that departure from the stabilised state, either in shear stress or VEGF concentration, led to neovascularisation [53]. A recent study estimated shear stress in endothelial cells in regions of neovascularisation [54]. The authors highlighted that values above 0.1 Pa were physiologically relevant.…”

Section: Introductionmentioning

confidence: 99%

“…Countless cardiovascular CFD models have enabled the quantification of variables which would prove challenging to measure in other settings [59,72]. For example, a CFD angiogenesis model estimated shear stresses experienced by endothelial tip cells [54]. A limitation of CFD is computational cost, with very sophisticated models requiring significant computing resources and time [73].…”

Section: Introductionmentioning

confidence: 99%

Computational Fluid Dynamics (CFD) Model for Analysing the Role of Shear Stress in Angiogenesis in Rheumatoid Arthritis

Motlana

Ngoepe

2023

IJMS

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Rheumatoid arthritis (RA) is an autoimmune disease characterised by an attack on healthy cells in the joints. Blood flow and wall shear stress are crucial in angiogenesis, contributing to RA’s pathogenesis. Vascular endothelial growth factor (VEGF) regulates angiogenesis, and shear stress is a surrogate for VEGF in this study. Our objective was to determine how shear stress correlates with the location of new blood vessels and RA progression. To this end, two models were developed using computational fluid dynamics (CFD). The first model added new blood vessels based on shear stress thresholds, while the second model examined the entire blood vessel network. All the geometries were based on a micrograph of RA blood vessels. New blood vessel branches formed in low shear regions (0.840–1.260 Pa). This wall-shear-stress overlap region at the junctions was evident in all the models. The results were verified quantitatively and qualitatively. Our findings point to a relationship between the development of new blood vessels in RA, the magnitude of wall shear stress and the expression of VEGF.

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Estimation of shear stress values along endothelial tip cells past the lumen of capillary sprouts

Cited by 7 publications

References 57 publications

Estimation of shear stress heterogeneity along capillary segments in angiogenic rat mesenteric microvascular networks

Estimation of shear stress heterogeneity along capillary segments in angiogenic rat mesenteric microvascular networks

Hemodynamic force dictates endothelial angiogenesis through MIEN1‐ERK/MAPK‐signaling axis

Computational Fluid Dynamics (CFD) Model for Analysing the Role of Shear Stress in Angiogenesis in Rheumatoid Arthritis

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