Estimation of Salivary Glucose and Glycogen Content in Exfoliated Buccal Mucosal Cells of Patients with Type II Diabetes Mellitus

Abstract

With the results of the present study it is revealed that salivary glucose and PAS positive cells are increased in diabetics which can be considered as adjuvant diagnostic tool for Diabetes mellitus.

Effect of diabetes mellitus type 2 on salivary glucose, immunoglobulin A, total protein, and amylase levels in adults: A systematic review and meta-analysis of case–control studies

“…The studies were published from 1996 to 2017. Two studies were reported in Turkey,[ 14 15 ] one in Pakistan,[ 16 ] three in Iran,[ 3 17 18 ] thirteen in India,[ 4 6 9 19 20 21 22 23 24 25 26 27 28 ] one in Brazil,[ 29 ] two in Nigeria,[ 30 31 ] one in Egypt,[ 5 ] one in Poland,[ 32 ] and one in China. [ 33 ] There were 1432 and 900 diabetic patients and healthy controls in all studies, respectively.…”

“…Nine studies measured the salivary profile of patients and controls in non-fasting condition (<2 h)[ 8 14 19 21 22 24 26 29 32 ] and 16 studies in fasting (8–12 h) condition. [ 3 4 5 6 9 15 16 17 20 23 25 27 28 30 31 33 ] Three studies reported SE,[ 15 17 27 ] one study reported median/range,[ 19 ] one study reported two means/SDs,[ 16 ] and a few studies reported different units, which were changed. Four studies[ 19 20 21 27 ] checked separately the factors in controlled diabetic and uncontrolled diabetic patients.…”

“…Ten studies[ 6 9 15 21 23 24 25 26 27 28 ] showed a correlation between salivary glucose and serum glucose levels in the diabetic patients, r value was found to be >0.75 in seven studies,[ 6 9 15 21 24 27 ] which was statistically significant ( P < 0.01), and two of which[ 22 28 ] checked this correlation in two diabetic groups. The r values in two studies were 0.394[ 23 ] and 0.54,[ 28 ] being found to be statistically significant ( P < 0.05). However, this correlation in two studies[ 25 26 ] was not statistically significant ( P > 0.05).…”

“…Several studies found a significant correlation between salivary and serum glucose levels in diabetic patients[ 6 9 15 21 23 24 27 28 ] and healthy controls. [ 6 9 21 27 ] Agrawal et al [ 36 ] reported an association between fasting saliva and fasting plasma glucose levels of diabetic patients and healthy controls.…”

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“…Later, glucose (1 gm/Kg body wt) orally fed to the rats and blood samples were collected at the different time interval (30', 60', 90', 120'). These samples were subjected to centrifugation at 300 g for 10 min and plasma was separated 21 . Glucose was estimated using GOD-POD based kit.…”