Role of bone marrow-derived mesenchymal stem cells on the parotid glands of streptozotocin induced diabetes rats

Denewar, Mona; Amin, Laila E

doi:10.1016/j.jobcr.2020.02.003

Cited by 13 publications

(6 citation statements)

References 21 publications

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“…In the context of this study's objective regarding the role of BMDSc on parotid glands of STZ‐induced diabetes rats, the statistical analysis of blood glucose levels revealed significant differences among the groups, confirming a reduction in blood glucose levels post‐BMDSc injection. This observation aligns with Fotino et al's findings, suggesting that BM‐MSC‐derived stem cell transplantation holds promise for tissue repair, regeneration, and immune response stimulation 30,31 …”

Section: Discussionsupporting

confidence: 87%

“…This observation aligns with Fotino et al's findings, suggesting that BM-MSC-derived stem cell transplantation holds promise for tissue repair, regeneration, and immune response stimulation. 30,31 In recent years, gene therapy has emerged as a potential treatment for diabetes. Lee et al 32 utilized a recombinant adeno-associated virus to deliver a single-chain insulin analog to the liver of diabetic rats induced by STZ.…”

Section: Discussionmentioning

confidence: 99%

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Ectopic expression of insulin in a type 1 diabetic rat model by injection of manipulated mesenchymal stem cells with an insulin construct driven by a glucose‐sensitive promoter in the port vein

Mohammad Dezashibi,

Farzad‐Mohajeri,

Bandehpour

et al. 2023

Cell Biochemistry & Function

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The treatment of type 1 diabetes through islet cell transplantation is a complex process, facing challenges such as allograft rejections and a limited supply of donors. One potential solution is to utilize the liver as an alternative for natural insulin production, as hepatocytes can secrete proteins and respond to glucose levels. Recent research has shown promising results in using mesenchymal stem cells as a potential cure for diabetes. The study utilized a diabetic rat model, confirmed through blood sugar measurement. A plasmid vector was designed with specific genetic components, synthesized by biotech company, and then Inserted vector into a plasmid with resistance genes and bacterial origin. Bone marrow‐derived mesenchymal stem cells (BM‐MSCs) were cultured and transfected with the plasmid using Lipofectamine 3000. Polymerase chain reaction was employed to confirm successful transfection using specific primers. For the animal study, 30 male Wistar rats were divided into six groups, each comprising five rats. The control group did not receive any treatment, while the second group received MSCs via Portal Vein Injection. The third group received MSCs transfected with a specific construct via Portal Vein Injection. The fourth group was induced to develop diabetes through streptozotocin (STZ) injection, the fifth group developed diabetes and received untransfected MSCs via Portal Vein Injection, and the sixth group received MSCs transfected with the specific construct via Portal Vein Injection. To manage Pain, appropriate pain control was administered to the rats for 3 days after the surgery. Fixed liver tissues obtained from the euthanized rats were utilized for immunohistochemistry. In this study, immunohistochemical techniques were used to examine insulin expression in different groups of rats. The control groups showed high levels of insulin expression, while the diabetic groups exhibited lower expression. However, there was a significant difference between the diabetic groups treated with MSC and transgenic MSC cells. All groups had similar baseline glucose levels, but the diabetic groups showed a significant increase after STZ injection, whereas the control and MSC groups did not. Postintervention, both the control and MSC groups had similar glucose levels to the post‐STZ levels. However, diabetes‐induced groups experienced a significant decrease in glucose levels, with the transfected MSCs showing a greater decrease than the untransfected MSCs. The study suggested that treatment with MSCs, especially transfected ones, can effectively reduce glucose levels in rats with diabetes. In this research, rat BM‐MSCs were utilized to create insulin‐producing mesenchymal cells with glucose‐sensitive insulin expression. The cells were transferred to the liver of diabetic rats via portal vein injection, leading to an increase in insulin expression. This study proposes a novel approach for cell therapy and delivery in the treatment of type 1 diabetes.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Ectopic expression of insulin in a type 1 diabetic rat model by injection of manipulated mesenchymal stem cells with an insulin construct driven by a glucose‐sensitive promoter in the port vein

Mohammad Dezashibi,

Farzad‐Mohajeri,

Bandehpour

et al. 2023

Cell Biochemistry & Function

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“…Studies have shown that MSCs interact with vascular endothelial cells to regulate inflammatory infiltration ( 44 ). On the other hand, bone marrow mesenchymal stem cells act through the release of soluble factors, so there are still many unknown areas to explore in cell therapy using mesenchymal stem cells ( 45 ).…”

Section: Discussionmentioning

confidence: 99%

Inhibitory Effect of Bovine Adipose-Derived Mesenchymal Stem Cells on Lipopolysaccharide Induced Inflammation of Endometrial Epithelial Cells in Dairy Cows

Liu

et al. 2021

Front. Vet. Sci.

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Endometritis is a disease that affects reproductive health in dairy cows and causes serious economic damage to the dairy industry world-wide. Although in recent years, the application of mesenchymal stem cell (MSC) therapy for the treatment of inflammatory diseases has attracted much attention, there are few reports of the use of MSCs in dairy cows. In the present study, our objective was to explore the inhibitory effects of bovine adipose-derived mesenchymal stem cells (bAD-MSCs) on lipopolysaccharide (LPS) induced inflammation in bovine endometrial epithelial cells (bEECs) along with the potential underlying molecular mechanisms. We characterized isolated bAD-MSCs using cell surface marker staining and adipogenic/osteogenic differentiation, and analyzed them using immunofluorescence, flow cytometry (surface marker staining), and adipogenic and osteogenic differentiation. Furthermore, to understand the anti-inflammatory effects of bAD-MSCs on LPS induced bEEC inflammation, we used a bAD-MSC/bEEC co-culture system. The results showed that bAD-MSC treatments could significantly decrease LPS induced bEEC apoptosis and pro-inflammatory cytokine expression levels, such as interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α). Furthermore, our results showed that bAD-MSC treatments could also significantly downregulate LPS induced p38, IkB-a, and JAK1 phosphorylation and Bax protein expression levels, which are closely related to inflammatory progress and cellular apoptosis in bEECs. Our findings demonstrate that bAD-MSCs play an inhibitory role in LPS induced bEEC inflammation and provide new insights for the clinical therapy of endometritis in dairy cows.

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“…AQP5 is also downregulated in the parotid salivary gland of T2D rats that showed decreased salivary flow rate [ 138 ]. Rats treatment with bone marrow-derived stem cells improves the salivary flow rate and alters AQP5 levels in the parotid gland [ 139 ]. It was also shown that autophagy degrades AQP5 in the submandibular salivary gland [ 46 ].…”

Section: Possible Role Of Aqps “Metabolic Network” In Obesity-induced Systemic Insulin Resistancementioning

confidence: 99%

Aquaporins in insulin resistance and diabetes: More than channels!

et al. 2021

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Aquaporins (AQPs) are part of the family of the integral membrane proteins. Their function is dedicated to the transport of water, glycerol, ammonia, urea, H 2 O 2 , and other small molecules across the biological membranes. Although for many years they were scarcely considered, AQPs have a relevant role in the development of many diseases. Recent discoveries suggest, that AQPs may play an important role in the process of fat accumulation and regulation of oxidative stress, two crucial aspects of insulin resistance and type-2 diabetes (T2D). Insulin resistance (IR) and T2D are multi-faceted systemic diseases with multiple connections to obesity and other comorbidities such as hypertension, dyslipidemia and metabolic syndrome. Both IR and T2D transcends different tissues and organs, creating the maze of mutual relationships between adipose fat depots, skeletal muscle, liver and other insulin-sensitive organs. AQPs with their heterogenous properties, distinctive tissue distribution and documented involvement in both the lipid metabolism and regulation of the oxidative stress appear to be feasible candidates in the search for the explanation to this third-millennium plague. A lot of research has been assigned to adipose tissue AQP7 and liver tissue AQP9, clarifying their relationship and coordinated work in the induction of hepatic insulin resistance. Novel research points also to other aquaporins, such as AQP11 which may be associated with the induction of insulin resistance and T2D through its involvement in hydrogen peroxide transport. In this review we collected recent discoveries in the field of AQP's involvement in the insulin resistance and T2D. Novel paths which connect AQPs with metabolic disorders can give new fuel to the research on obesity, insulin resistance and T2D - one of the most worrying problems of the modern society.

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Role of bone marrow-derived mesenchymal stem cells on the parotid glands of streptozotocin induced diabetes rats

Cited by 13 publications

References 21 publications

Ectopic expression of insulin in a type 1 diabetic rat model by injection of manipulated mesenchymal stem cells with an insulin construct driven by a glucose‐sensitive promoter in the port vein

Ectopic expression of insulin in a type 1 diabetic rat model by injection of manipulated mesenchymal stem cells with an insulin construct driven by a glucose‐sensitive promoter in the port vein

Inhibitory Effect of Bovine Adipose-Derived Mesenchymal Stem Cells on Lipopolysaccharide Induced Inflammation of Endometrial Epithelial Cells in Dairy Cows

Aquaporins in insulin resistance and diabetes: More than channels!

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