Estimation of Lecithin in Rabbit Tissues

Abstract

1. A simple method for lecithin estimation was developed. Phospholipids in rabbit tissues were extracted with chloroform and methanol. 2. The samples were chromatographed with eluting solvent system; chloroform:methanol:glacial acetic acid:water (100:25:8:1.1). Lecithin was identified by the spray reagent as blue spots and the intensities were quantitated with a densitometer. 3. The concentration of lecithin in the various rabbit tissues varied from 2.8 to 10 mg/g tissue. The sequence of increasing lecithin concentration was heart less than kidney less than lung less than liver less than brain. 4. Recovery experiments of added lecithin into the tissues showed a recovery of 91-104%.

UPTAKE OF 1:2-14C-CHOLINE BY THE GUINEA-PIG LUNG IN VITRO: AND THE EFFECTS OF P-TERPHENYL HEMICHOLINIUM-3

“…Contact time with the film was 4 weeks, after which the films were processed at 21 "C. The chromatograms were then sprayed with the phosphorus reagent (Gardiner & Lee, 1982) to locate the various phospholipids.…”

“…The extraction of phospholipids and the assay of lecithin were carried out according to the method of Gardiner & Lee (1982).…”

“…Paper chromatograms of the phospholipid extracts containing reference indicators of lecithin, sphingomyelin were labelled with radioactive ink and placed in contact with X-ray film. Contact time with the film was 4 weeks, after which the films were processed at 21 "C. The chromatograms were then sprayed with the phosphorus reagent (Gardiner & Lee, 1982) to locate the various phospholipids.…”