Estimation of Leaf Chlorophyll a, b and Carotenoid Contents and Their Ratios Using Hyperspectral Reflectance

Sonobe, Rei; Yamashita, Hiroto; Mihara, Harumi; Morita, Akio; Ikka, Takashi

doi:10.3390/rs12193265

Cited by 56 publications

(39 citation statements)

References 88 publications

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“…This effect resulted from the substantial increase in chlorophyll b caused by the latter treatment, which was not supported by an increase in chlorophyll a. The ratio chlorophyll a/chlorophyll b has been proposed as a parameter to evaluate crops response to environmental adversities, diseases, and stresses [ 70 ]. In particular, some stressors can provoke the conversion of the chlorophyll a to b via the enzyme activity of Chl a oxygenase [ 71 ].…”

Section: Discussionmentioning

confidence: 99%

Lignin Nanoparticles: A Promising Tool to Improve Maize Physiological, Biochemical, and Chemical Traits

2021

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Lignin, and its derivatives, are the subject of current research for the exciting properties shown by this biomass. Particularly attractive are lignin nanoparticles for their eco- and biocompatibility compared to other nanomaterials. In this context, the effect of nanostructured lignin microparticles (LNP), obtained from alkaline lignin by acid treatment, on maize plants was investigated. To this end, maize seeds were primed with LNP at five concentrations: 80 mg L−1 (T80), 312 mg L−1 (T312), 1250 mg L−1 (T1250), 5000 mg L−1 (T5000) and 20,000 mg L−1 (T20000). Concerning the dose applied, LNP prompted positive effects on the first stages of maize development (germination and radicle length). Furthermore, the study of plant growth, biochemical and chemical parameters on the developed plants indicated that concerning the dose applied. LNP stimulated beneficial effects on the seedlings (fresh weight and length of shoots and roots). Besides, specific treatments increased the content of chlorophyll (a and b), carotenoid, and anthocyanin. Finally, the soluble protein content showed a positive trend in response to specific dosages. These effects are significant, given the essential biological function performed by these biomolecules. In conclusion, this research indicates as the nanostructured lignin microparticles can be used, at appropriate dosages, to induce positive biological responses in maize. This beneficial action deserves attention as it candidates LNP for biostimulating a crop through seed priming.

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Section: Discussionmentioning

confidence: 99%

Lignin Nanoparticles: A Promising Tool to Improve Maize Physiological, Biochemical, and Chemical Traits

2021

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“…Also, the proximal measurement of original reflectance and data preprocessing procedures support the in situ hyperspectral analysis of the leaves of Eutrema japonicum, called the Japanese horseradish or wasabi, to assay chlorophyll and carotenoids as biomarkers of the plant stress in response to changes in pH and sulfur. This procedure has been thus proposed as a suitable tool to support a proper greenhouse cultivation of highly sensitive plants, particularly helpful for inexpert farmers [39]. Chlorophyll fluorescence can be also exploited as indirect biomarker for the in situ optical measurement of anthocyanins in grape [40] (Figure 6).…”

Section: Chlorophyll As Photophysical Biomarkermentioning

confidence: 99%

Light and Autofluorescence, Multitasking Features in Living Organisms

Croce

2021

Photochem

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Organisms belonging to all life kingdoms may have the natural capacity to fluoresce. Autofluorescence events depend on the presence of natural biomolecules, namely endogenous fluorophores, with suitable chemical properties in terms of conjugated double bonds, aromatic or more complex structures with oxidized and crosslinked bonds, ensuring an energy status able to permit electronic transitions matching with the energy of light in the UV-visible-near-IR spectral range. Emission of light from biological substrates has been reported since a long time, inspiring unceasing and countless studies. Early notes on autofluorescence of vegetables have been soon followed by attention to animals. Investigations on full living organisms from the wild environment have been driven prevalently by ecological and taxonomical purposes, while studies on cells, tissues and organs have been mainly promoted by diagnostic aims. Interest in autofluorescence is also growing as a sensing biomarker in food production and in more various industrial processes. The associated technological advances have supported investigations ranging from the pure photochemical characterization of specific endogenous fluorophores to their possible functional meanings and biological relevance, making fluorescence a valuable intrinsic biomarker for industrial and diagnostic applications, in a sort of real time, in situ biochemical analysis. This review aims to provide a wide-ranging report on the most investigated natural fluorescing biomolecules, from microorganisms to plants and animals of different taxonomic degrees, with their biological, environmental or biomedical issues relevant for the human health. Hence, some notes in the different sections dealing with different biological subject are also interlaced with human related issues. Light based events in biological subjects have inspired an almost countless literature, making it almost impossible to recall here all associated published works, forcing to apologize for the overlooked reports. This Review is thus proposed as an inspiring source for Readers, addressing them to additional literature for an expanded information on specific topics of more interest.

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“…Therefore, the modeling accuracy was not high. The use of machine learning algorithms is an effective way to express the complex relationship between the modeling factors and dependent variables (Saitta et al, 2011;Sonobe et al, 2021). The back propagation (BP) approach has been used to determine plant diseases (Saleem et al, 2019), and this methodology has potential in the analysis of hyperspectral reflectance data.…”

Section: Introductionmentioning

confidence: 99%

Using Spectral Reflectance to Estimate the Leaf Chlorophyll Content of Maize Inoculated With Arbuscular Mycorrhizal Fungi Under Water Stress

et al. 2021

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Leaf chlorophyll content is an important indicator of the growth and photosynthesis of maize under water stress. The promotion of maize physiological growth by (AMF) has been studied. However, studies of the effects of AMF on the leaf chlorophyll content of maize under water stress as observed through spectral information are rare. In this study, a pot experiment was carried out to spectrally estimate the leaf chlorophyll content of maize subjected to different durations (20, 35, and 55 days); degrees of water stress (75%, 55% and 35% water supply) and two inoculation treatments (inoculation with Funneliformis mosseae and no inoculation). Three machine learning algorithms, including the back propagation (BP) method, least square support vector machine (LSSVM) and random forest (RF) method, were used to estimate the leaf chlorophyll content of maize. The results showed that AMF increased the leaf chlorophyll content, net photosynthetic rate (A), stomatal conductance (gs), transpiration rate (E), and water use efficiency (WUE) of maize but decreased the intercellular carbon dioxide concentration (Ci) of maize and atmospheric vapor pressure deficit (VPD) regardless of the water stress duration and degree. The first-order differential spectral data can better reflect the correlation between leaf chlorophyll content and spectrum of inoculated maize when compared with original spectral data. The BP model performed bestin modeling the maize leaf chlorophyll content, yielding the largest R2-values and smallest root mean square error (RMSE) values, regardless of stress duration. These results provide a reliable basis for the effective monitoring of the leaf chlorophyll content of maize under water stress.

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Estimation of Leaf Chlorophyll a, b and Carotenoid Contents and Their Ratios Using Hyperspectral Reflectance

Cited by 56 publications

References 88 publications

Lignin Nanoparticles: A Promising Tool to Improve Maize Physiological, Biochemical, and Chemical Traits

Lignin Nanoparticles: A Promising Tool to Improve Maize Physiological, Biochemical, and Chemical Traits

Light and Autofluorescence, Multitasking Features in Living Organisms

Using Spectral Reflectance to Estimate the Leaf Chlorophyll Content of Maize Inoculated With Arbuscular Mycorrhizal Fungi Under Water Stress

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