Estimation of Growth Rate from the Mitotic Index

Chung, King‐Thom; Nilson, E.H.; Case, M.J.; Marr, Allen G.; Hungate, R. E.

doi:10.1128/aem.25.5.778-780.1973

Cited by 4 publications

(2 citation statements)

References 2 publications

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“…d P value for trend was calculated in the generalized estimating equation model including stage as an ordinal variable. marker for tumor growth, 33 our finding suggests that amelanotic melanomas grow faster than pigmented melanomas. Previous studies variably found ulceration to be more frequent in 14,34 or not associated with 6 amelanotic melanoma.…”

Section: Discussionmentioning

confidence: 50%

Comparison of Clinicopathologic Features and Survival of Histopathologically Amelanotic and Pigmented Melanomas

et al. 2014

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Importance Previous studies have reported that histopathologically amelanotic melanoma is associated with poorer survival than pigmented melanoma; however, small numbers of amelanotic melanomas, selected populations, lack of centralized pathology review, or no adjustment for stage limit interpretation or generalization of results from prior studies. Objective To compare melanoma-specific survival between patients with histopathologically amelanotic and those with pigmented melanoma in a large international population-based study. Design Survival analysis with median follow-up of 7.6 years. Setting The Genes, Environment, and Melanoma study enrolled incident cases of melanoma diagnosed in 1998-2003 from international population-based cancer registries. Participants A total of 2,995 patients with 3,486 invasive primary melanomas centrally scored for histologic pigmentation. Main Outcomes and Measurements Clinicopathologic predictors and melanoma-specific survival of histologically amelanotic and pigmented melanoma were compared using generalized estimating equations and Cox regression models, respectively. Results Eight percent of melanomas (275 of 3,467) were histopathologically amelanotic. Female sex, nodular and unclassified or other histologic subtypes, increased Breslow thickness, presence of mitoses, severe solar elastosis, and lack of a co-existing nevus were independently associated with amelanotic melanoma (each P < .05). Amelanotic melanoma was generally of a higher American Joint Committee on Cancer (AJCC) tumor stage at diagnosis (P for trend <.001) than pigmented melanoma. Hazard of death from melanoma was higher for amelanotic than pigmented melanoma [hazard ratio (HR), 2.0; 95% confidence interval (CI), 1.4-3.0; P< .001], adjusted for age, sex anatomic site, and study design variables; but survival did not differ once AJCC tumor stage was also taken into account, (HR, 0.8; 95% CI, 0.5-1.2; P = .36). Conclusions and Relevance At the population level, survival after diagnosis of amelanotic melanoma is poorer than after pigmented melanoma because of its more advanced stage at diagnosis. It is probable that amelanotic melanomas present at more advanced tumor stages because they are difficult to diagnose. The association of amelanotic melanoma with presence of mitoses independently of Breslow thickness and other clinicopathologic characteristics suggests that amelanotic melanomas might also grow faster than pigmented melanomas. New strategies for early diagnosis and investigation of the biology of amelanotic melanoma are warranted.

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Section: Discussionmentioning

confidence: 50%

Comparison of Clinicopathologic Features and Survival of Histopathologically Amelanotic and Pigmented Melanomas

et al. 2014

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“…The theoretical considerations involved in relating FDC to growth rate have been discussed by a number of authors (e.g., 1,4,17). Briefly, for a population of microorganisms experiencing asynchronous steady-state growth, ,u = (lltd)[loge (1 + J)] (17) where ,u is the growth rate, td is the average duration of mitosis, and f is the fraction of dividing cells (FDC/100) in the population.…”

Section: Resultsmentioning

confidence: 99%

Double-Staining Epifluorescence Technique to Assess Frequency of Dividing Cells and Bacteriovory in Natural Populations of Heterotrophic Microprotozoa

Sherr

1983

Appl Environ Microbiol

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We have developed a double-staining procedure for use with epifluorescence microscopy which allows the detection both of dividing cells and of ingested bacteria in food vacuoles of heterotrophic microprotozoa. Microprotozoan cells are stained sequentially with the DNA-specific fluorochrome DAPI (4',6-diamidino-2-phenylindole) and the nonspecific protein stain fluorescein isothiocyanate. During microscopic examination, heterotrophic microprotozoan cells are first located with fluorescein isothiocyanate fluorescence and then epifluorescence filter sets are switched to permit inspection under DAPI fluorescence of the cell nuclei and of the contents of food vacuoles. Among in situ populations of estuarine microprotozoa sampled over a tidal cycle, we found from 2.2 to 5.2% of the heterotrophic cells in a recognizable stage of division (nuclei elongated or double). Batch culture growth experiments were also carried out both with natural populations and with two isolated species of estuarine microprotozoa. In these experiments, the frequency of dividing cells ranged from 1.2 to 3.8% and appeared to be negatively correlated with growth rate. Microprotozoan populations sampled in continental shelf waters off Savannah, Ga., had mean frequencies of dividing cells ranging from 2.0 to 5.0%. A large fraction of cells in heterotrophic microprotozoan populations (an average of 27.4 ± 1.0% in estuarine water and of 30.1 ± 4.8% in shelf water) had DAPI-stained inclusions, presumably recently ingested bacteria, in their food vacuoles.

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Coarse-grained modeling of cell division in 3D: influence of density, medium viscosity, and inter-membrane friction on cell growth and nearest neighbor distribution

et al. 2020

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Estimation of Growth Rate from the Mitotic Index

Cited by 4 publications

References 2 publications

Comparison of Clinicopathologic Features and Survival of Histopathologically Amelanotic and Pigmented Melanomas

Comparison of Clinicopathologic Features and Survival of Histopathologically Amelanotic and Pigmented Melanomas

Double-Staining Epifluorescence Technique to Assess Frequency of Dividing Cells and Bacteriovory in Natural Populations of Heterotrophic Microprotozoa

Coarse-grained modeling of cell division in 3D: influence of density, medium viscosity, and inter-membrane friction on cell growth and nearest neighbor distribution

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