Estimation of genomic instability and mutation induction by graphene oxide nanoparticles in mice liver and brain tissues

Mohamed, Hanan R. H.; Welson, Mary; Yaseen, Ahmed E.; El-Ghor, Akmal A.

doi:10.1007/s11356-019-06930-0

Cited by 17 publications

(17 citation statements)

References 39 publications

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“…Therefore, a recent study demonstrated that after injection of GO at different doses (10, 20, and 40 mg/kg) for one or five consecutive days, it caused genomic instability, mutagenicity, and oxidative stress in the liver and brain tissue. Besides, administration of GO significantly increased dose-dependent DNA breaks and induced mutations in the p53 (6 and 7) and presenilin (exon 5) genes by increasing the expression of the p53 protein [ 135 ].…”

Section: Toxicity Of Go In Vivomentioning

confidence: 99%

Synthesis and Toxicity of Graphene Oxide Nanoparticles: A Literature Review of In Vitro and In Vivo Studies

Rhazouani

Gamrani

Achaby

et al. 2021

BioMed Research International

107

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Nanomaterials have been widely used in many fields in the last decades, including electronics, biomedicine, cosmetics, food processing, buildings, and aeronautics. The application of these nanomaterials in the medical field could improve diagnosis, treatment, and prevention techniques. Graphene oxide (GO), an oxidized derivative of graphene, is currently used in biotechnology and medicine for cancer treatment, drug delivery, and cellular imaging. Also, GO is characterized by various physicochemical properties, including nanoscale size, high surface area, and electrical charge. However, the toxic effect of GO on living cells and organs is a limiting factor that limits its use in the medical field. Recently, numerous studies have evaluated the biocompatibility and toxicity of GO in vivo and in vitro. In general, the severity of this nanomaterial’s toxic effects varies according to the administration route, the dose to be administered, the method of GO synthesis, and its physicochemical properties. This review brings together studies on the method of synthesis and structure of GO, characterization techniques, and physicochemical properties. Also, we rely on the toxicity of GO in cellular models and biological systems. Moreover, we mention the general mechanism of its toxicity.

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Section: Toxicity Of Go In Vivomentioning

confidence: 99%

Synthesis and Toxicity of Graphene Oxide Nanoparticles: A Literature Review of In Vitro and In Vivo Studies

Rhazouani

Gamrani

Achaby

et al. 2021

BioMed Research International

107

View full text Add to dashboard Cite

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“…Thirteen studies, published in seven papers, assessed the in vivo genotoxicity of graphene ( Table 7 ). Among them, ten studied the effect of GO [ 60 , 96 , 97 , 98 , 99 ], two of rGO [ 97 , 100 ] and one of exfoliated graphene [ 91 ]. Regarding graphene material characterization, it was not found in two of the publications; in the others, the amount of information was not the same among different studies but, in general, lateral size, z-potential, and hydrodynamic diameter are provided.…”

Section: Resultsmentioning

confidence: 99%

Genotoxicity of Graphene-Based Materials

et al. 2022

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Graphene-based materials (GBMs) are a broad family of novel carbon-based nanomaterials with many nanotechnology applications. The increasing market of GBMs raises concerns on their possible impact on human health. Here, we review the existing literature on the genotoxic potential of GBMs over the last ten years. A total of 50 articles including in vitro, in vivo, in silico, and human biomonitoring studies were selected. Graphene oxides were the most analyzed materials, followed by reduced graphene oxides. Most of the evaluations were performed in vitro using the comet assay (detecting DNA damage). The micronucleus assay (detecting chromosome damage) was the most used validated assay, whereas only two publications reported results on mammalian gene mutations. The same material was rarely assessed with more than one assay. Despite inhalation being the main exposure route in occupational settings, only one in vivo study used intratracheal instillation, and another one reported human biomonitoring data. Based on the studies, some GBMs have the potential to induce genetic damage, although the type of damage depends on the material. The broad variability of GBMs, cellular systems and methods used in the studies precludes the identification of physico-chemical properties that could drive the genotoxicity response to GBMs.

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“…Previous studies have shown that the accumulation of succinic acid, an intermediate substance in the TCA cycle, can stimulate inflammation and induce upregulation of inflammatory factors (Tannahill et al, 2013). Metabolomics data showed that MSNs injection led to a significant increase in succinic acid, indicating that it is related to inflammation and inflammatory factors (Lim et al, 2019;Mohamed et al, 2020). Moreover, liver injury triggers the repair and regeneration of liver cells.…”

Section: Discussionmentioning

confidence: 97%

“…Moreover, oral administration (IG) of MSN suspensions to ICR mice at a dose of 40 mg/kg and spherical MSNs yielded high liver accumulation after 7 days. Mohamed et al found that acute and subacute oral administration of graphene oxide nanoparticles induced genomic instability and mutagenicity in the mouse liver (Mohamed et al, 2020), which are closely involved in oxidative stress.…”

Section: Introductionmentioning

confidence: 99%

Integrative Metabolomics, Proteomics and Transcriptomics Analysis Reveals Liver Toxicity of Mesoporous Silica Nanoparticles

Sun

et al. 2022

Front. Pharmacol.

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As pharmaceutical excipients, mesoporous silica nanoparticles (MSNs) have attracted considerable concern based on potential risks to the public. The impact of MSNs on biochemical metabolism is poorly understood, and few studies have compared the effects of MSNs administered via different routes. To evaluate the hepatotoxicity of MSNs, metabolomics, proteomics and transcriptomic analyses were performed in mice after intravenous (20 mg/kg/d) or oral ad-ministration (200 mg/kg/d) of MSNs for 10 days. Intravenous injection induced significant hepatic injury based on pathological inspection and increased the levels of AST/ALT and the inflammatory factors IL-6, IL-1β and TNF-a. Omics data suggested intravenous administration of MSNs perturbed the following metabolites: succinate, hypoxanthine, GSSG, NADP+, NADPH and 6-phosphogluconic acid. In addition, increases in GPX, SOD3, G6PD, HK, and PFK at proteomic and transcriptomic levels suggested elevation of glycolysis and pentose phosphate pathway, synthesis of glutathione and nucleotides, and antioxidative pathway activity, whereas oxidative phosphorylation, TCA and mitochondrial energy metabolism were reduced. On the other hand, oral administration of MSNs disturbed inflammatory factors and metabolites of ribose-5-phosphate, 6-phosphogluconate, GSSG, and NADP+ associated with the pentose phosphate pathway, glutathione synthesis and oxidative stress albeit to a lesser extent than intravenous injection despite the administration of a ten-fold greater dose. Overall, systematic biological data suggested that intravenous injection of nanoparticles of pharmaceutical excipients substantially affected hepatic metabolism function and induced oxidative stress and inflammation, whereas oral administration exhibited milder effects compared with intravenous injection.

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Estimation of genomic instability and mutation induction by graphene oxide nanoparticles in mice liver and brain tissues

Cited by 17 publications

References 39 publications

Synthesis and Toxicity of Graphene Oxide Nanoparticles: A Literature Review of In Vitro and In Vivo Studies

Synthesis and Toxicity of Graphene Oxide Nanoparticles: A Literature Review of In Vitro and In Vivo Studies

Genotoxicity of Graphene-Based Materials

Integrative Metabolomics, Proteomics and Transcriptomics Analysis Reveals Liver Toxicity of Mesoporous Silica Nanoparticles

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