Estimation of gamma globulin in the serum of patients with hypogammaglobulinaemia

Abstract

Three methods of estimating γ globulin in the serum of patients with hypogammaglobulinaemia are described and compared, namely free electrophoresis, the inhibition of antiglobulin antiserum method, and the gel diffusion precipitin method. Data from 142 parallel estimations are reported.The errors of these methods are determined.The results of the three techniques give good general agreement. This is due to the fact that the 7S component dominates the estimations whichever technique is used. Some discrepancies are discussed.All of the techniques are applicable to the diagnosis of hypogammaglobulinaemia. Their practical limitations are discussed.

The Role of 19S Gamma‐Globulin Blood‐Group Antibodies in the Antiglobulin Reaction

“…These workers used a slightly diffcrcnt immunological mcthod, namely precipitation of protein with a suitablc antiserum, followed by assay of tlie nitrogen content of the precipitate. On the other hand, Dcrrington and Soothill (1961), using the gel-diffusion precipitin method, obtained distinctly higher results for the amount of 19s y globulin in cord seruma discrepancy which has not yet been cxplained.…”

Pernicious Anaemia, Myxoedema, and Hypogammaglobulinaemia--A Family Study

“…The M.R.C. working party accepted a level of less than 200 mg. for the diagnosis of hypogammaglobulinaemia (Kekwick, Vallet, Cutbush, Mollison, Thomas, Gell, and Soothill, 1961). Primary and secondary varieties of the condition are recognized.…”

A Family with Several Cases of Hypogammaglobulinaemia

“…Later (December 1958), in two samples examined by Dr. J. F. Soothill, the levels were shown to be 80 and 60 mg./100 ml. (method described by Gell, 1957, and by Kekwick, Vallet, Cutbush, Mollison, Thomas, Gell and Soothill. 1961).…”