Estimation of direct and indirect gas production in syringes: A tool to estimate short chain fatty acid production that requires minimal laboratory facilities

Fievez, Veerle; Babayemi, O. J.; Demeyer, Daniël

doi:10.1016/j.anifeedsci.2005.05.001

Cited by 120 publications

(86 citation statements)

References 14 publications

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“…High fermentation rates indicate high nutrient availability for rumen microorganisms. Besides, a high NDF level in feedstuff may be because of a low fermentation rate (Fievez et al, 2005). The T 1/2 value of J. acutus stems calculated from the Ørskov & McDonald (1979) model was higher than that of J. acutus seeds, though the T 1/2 value of J. acutus stems was similar to those reported for wheat straw, oat straw and berseem by Sing et al (2010).…”

Section: Resultssupporting

confidence: 51%

Estimation of organic matter digestibility, metabolizable energy, phenolic compounds and antioxidant activity of stems and seeds of the <i>Juncus acutus</i> plant in ruminant nutrition

Erdem¹,

Çetinkaya²,

Nisbet³

et al. 2016

SA J. An. Sci.

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The objectives of the present study were to determine the organic matter digestibility (OMD), metabolizable energy (ME), antioxidant activity and total phenolic and flavonoid concentrations of stems and seeds of Juncus acutus. Stem and seed samples were collected from Hamsiloz Bay in Sinop, Turkey, and the proximate analysis was carried on them. The OMD percentage and ME values of the samples were estimated from gas measured by the in vitro gas production method. Phenolic and flavonoid concentrations and total antioxidant activity were determined spectrophotometrically. Mean OMD, ME OMD and ME GP levels and gas production kinetic parameters A, c and T 1/2 of J. acutus stem and seed samples were 40.3% and 47.7%; 6.44 and 7.63 MJ/kg DM, 5.96 and 7.07 MJ/kg DM, 35.12 and 47.19 mL, 0.055% and 0.092%, and 12.60 and 7.53 h, respectively. Mean OMD percentage, ME and gas production kinetic parameters A, c and T 1/2 of the stems were significantly different than that of the seed. The antioxidant activity, total phenolic and flavonoid concentrations of stems and seeds of J. acutus were 88.45 and 88.48 IC50 mg/mL, 19.70 and 40.99 mg GAE/100 g and 0.63 and 1.20 mg Qe/100 g, respectively. Mean values of total phenolic and flavonoid concentrations of stems were significantly different from that of the seeds. In conclusion, both stems and seeds of J. acutus may be considered alternative feed sources for ruminants. Furthermore, when J. acutus stems and seeds are included in ruminant diet, the phenolic compounds may contribute to the intake of natural antioxidants.

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Section: Resultssupporting

confidence: 51%

Estimation of organic matter digestibility, metabolizable energy, phenolic compounds and antioxidant activity of stems and seeds of the <i>Juncus acutus</i> plant in ruminant nutrition

Erdem¹,

Çetinkaya²,

Nisbet³

et al. 2016

SA J. An. Sci.

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“…Venting the gas produced requires that CH 4 concentration is measured simultaneously, given that different CH 4 concentrations can be expected depending on substrate or stage of incubation. Although the most common method to measure CH 4 concentration on gas samples collected from incubation vessels is by using gas chromatography, as for in vivo measurements, other techniques are available and have been used such as infrared methane analysis (Goel et al, 2008;Cobellis et al, 2015) and absorption of CO 2 (Fievez et al, 2005).…”

Section: Incubation Procedures and Ch 4 Measurementsmentioning

confidence: 99%

Design, implementation and interpretation of in vitro batch culture experiments to assess enteric methane mitigation in ruminants—a review

Yáñez-Ruíz

Bannink

Dijkstra

et al. 2016

Animal Feed Science and Technology

130

120

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a b s t r a c tIn vitro fermentation techniques (IVFT) have been widely used to evaluate the nutritive value of feeds for ruminants and in the last decade to assess the effect of different nutritional strategies on methane (CH4) production. However, many technical factors may influence the results obtained. The present review has been prepared by the 'Global Network' FACCE-JPI international research consortium to provide a critical evaluation of the main factors that need to be considered when designing, conducting and interpreting IVFT experiments that investigate nutritional strategies to mitigate CH 4 emission from ruminants. Given the increasing and wide-scale use of IVFT, there is a need to critically review reports in the literature and establish what criteria are essential to the establishment and implementation of in vitro techniques. Key aspects considered include: i) donor animal species and number of animal used, ii) diet fed to donor animals, iii) collection and processing of rumen fluid as inoculum, iv) choice of substrate and incubation buffer, v) incubation procedures and CH 4 measurements, vi) headspace gas composition and vii) comparability of in vitro and in vivo measurements. Based on an evaluation of experimental evidence, a set of technical recommendations are presented to harmonize IVFT for feed evaluation, assessment of rumen function and CH 4 production.

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“…The OPF was incubated in gas-tight 100 mL, plastic syringes containing 20 mL of a phosphatebicarbonate buffer adjusted to a pH of 6.8 (Fievez et al, 2005) and 5 mL of rumen content. Then, all air was expelled from the syringes, after which their tips were closed.…”

Section: Collection and Preparation Of Samplesmentioning

confidence: 99%

The Effects of Adding Lactic Acid Bacteria and Cellulase in Oil Palm(Elais GuineensisJacq.) Frond Silages on Fermentation Quality, Chemical Composition and in Vitro Digestibility

Ebrahimi

Rajion

Goh

et al. 2014

Italian Journal of Animal Science

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The main objective of the current study was to evaluate whether oil palm frond (OPF) can be successfully ensiled without or with the additives cellulase or lactic acid bacteria (LAB). Thus, fresh OPF was ensiled either without additives or with cellulase or LAB or their combination. Ensiling was carried out by storing 2 kg samples in airtight glass jars at 25-30°C for 12 weeks. Thereafter, the silage samples were subjected to proximate analyses, an in vitro digestibility assay and measures on selected indices of fermentation. Fermentation of OPF without additives appeared to be unsuccessful as both pH and ammonia content were too high (4.9 and 9.9%, respectively). In contrast, the use of cellulase or LAB resulted in silages with a pH<4.5 and ammonia fractions <8.4%, but the lowest values were found when both cellulase and LAB were used, i.e. pH=4.1 and ammonia fraction=6.7%. In vitro digestibility of dry matter was significantly higher in the cellulase treated silages. The process of ensiling was associated with both a significant decrease of the fat content of OPF and a significant change of the fatty acid profile. However, the proportions of major fatty acids (C16:0 and C18:2n-6) were not affected by the process of ensiling. In conclusion, the use of cellulase additive appears a practical tool to safeguard the process of fermentation. Using a cellulase enzyme or its combination with LAB improves the fermentation profile and increases the nutritional value of the OPF silage.

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Estimation of direct and indirect gas production in syringes: A tool to estimate short chain fatty acid production that requires minimal laboratory facilities

Cited by 120 publications

References 14 publications

Estimation of organic matter digestibility, metabolizable energy, phenolic compounds and antioxidant activity of stems and seeds of the <i>Juncus acutus</i> plant in ruminant nutrition

Estimation of organic matter digestibility, metabolizable energy, phenolic compounds and antioxidant activity of stems and seeds of the <i>Juncus acutus</i> plant in ruminant nutrition

Design, implementation and interpretation of in vitro batch culture experiments to assess enteric methane mitigation in ruminants—a review

The Effects of Adding Lactic Acid Bacteria and Cellulase in Oil Palm(Elais GuineensisJacq.) Frond Silages on Fermentation Quality, Chemical Composition and in Vitro Digestibility

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