Estimation of body surface area in the musk shrew (<i>Suncus murinus</i>): a small animal for testing chemotherapy-induced emesis

Eiseman, Julie L.; Sciullo, Michael; Wang, Hong; Beumer, Jan H.; Horn, Charles C.

doi:10.1177/0023677217695851

Cited by 4 publications

(5 citation statements)

References 15 publications

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“…[12][13][14][15] Since 1950, Meeh formula, 16 which allows translation between body weight (BW) based doses in animals and doses in humans on a body surface area (BSA) equivalent basis, has been widely used in clinical work and pharmacological research. [17][18][19][20][21] This study measured the BSA of tree shrews by image analysis, and the Meeh constant (Km = BSA/BW 2/3 ) was determined to facilitate equivalent dose conversion between tree shrews and other species.…”

Section: Introductionmentioning

confidence: 99%

Body surface area-based equivalent dose calculation in tree shrew

et al. 2021

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Tree shrew (Tupaia belangeri) is a promising experimental animal in biomedical research, but the equivalent doses of drugs between tree shrew and human and other animals has not been explored, which hinders its further application in a wider scope. The main objective of this article is to provide a method of equivalent dose conversion between tree shrews and other species based on body surface area (BSA). BSA of tree shrews were measured by Image J software, and then the average Km value of tree shrews was figured out based on the body weights and BSA, then the conversion coefficients of equivalent dose among tree shrew and other species of experimental animals were calculated based known data. The Km value of tree shrews was 0.105 ± 0.001. Through BSA conversion, the equivalent dose for tree shrews (D-ts) relative to rats was obtained by formula: D-ts = 1.36 × D-a (rats weighing 200 g as example), and the error was less than 10% when the BW of the tree shrew was 0.09 kg–0.15 kg. The coefficients of equivalent dose transferring from tree shrews to human and other species were calculated in article. These parameters could be used to determine a suitable dosing strategy for tree shrew studies.

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Section: Introductionmentioning

confidence: 99%

Body surface area-based equivalent dose calculation in tree shrew

et al. 2021

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“…In contrast, the equivalent dose calculated by BSA conversion (62 mg/kg) could achieve an anesthetic time of 44.28 AE 3.95 min, with no serious or fatal effects, indicating that BSA is an appropriate parameter for determining the drug dosage for different species. [22][23][24][25] We recognized that our study also had a number of limitations, one of which was that this protocol lacked the use of any analgesia, thus making it applicable only in non-painful procedures or minor procedures with the addition of an analgesic agent. The main purpose of the current study was to verify the validity of the equivalent dose of sodium pentobarbital for tree shrews, which would eliminate the need for additional examinations after recovery (e.g.…”

Section: Discussionmentioning

confidence: 99%

Dosage selection and effect evaluation of sodium pentobarbital in tree shrew anesthesia

et al. 2023

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To achieve surgical anesthesia in animal experimentation, it is important to select the appropriate anesthetic dose. However, few studies have investigated the reasonable anesthetic dose in tree shrew ( Tupaia belangeri). The aim of the study was to review the literature to determine the most commonly used anesthetic dose in tree shrew and to calculate the reasonable equivalent dose between tree shrew and rat based on the body surface area conversion. Two groups of 10 adult tree shrews each were anesthetized with 1% sodium pentobarbital through intraperitoneal injection separately at doses of 62 mg/kg (equivalent dose) and 40 mg/kg (reported dose). Anesthetic depth and times were assessed in addition to vital signs. The results showed that the dosage was quite different across studies, ranging from 15 mg/kg to 80 mg/kg, with 40 mg/kg being the most frequently reported dose. However, the group of tree shrews anesthetized with the commonly reported dose were unable to meet the requirements of surgery. In contrast, the equivalent dose (62 mg/kg, intraperitoneal injection with sodium pentobarbital) calculated by body surface area conversion could achieve an anesthetic time of 44.28 ± 3.95 min with no serious or fatal effects. During anesthetic monitoring, we found that sodium pentobarbital had an inhibitory effect on the blood pressure, pulse rate, respiratory rate and rectal temperature in tree shrews, especially on the respiratory rate. Thus, our study indicated that the use of the equivalent dose of sodium pentobarbital was effective in anesthetizing tree shrews.

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“…In the second experiment, male C57BL6/J and Kit W-sh/W-sh mice were administered intraperitoneally with a single dose of LPS (4 mg/kg) diluted in saline solution (80 μg LPS in 1 ml saline). This dose of LPS has been demonstrated to elicit moderate brain inflammation according to body surface area conversion equation [24,25] without compromising the survival (LD50 value of LPS in mice, 20 mg/kg) [26]. The mice were divided into the following four treatment groups: wild-type (WT)/control group (WT mice+saline, n = 6); WT/LPS group (WT mice+LPS ip-injection, n = 6); Kit W-sh/W-sh /control group (Kit W-sh/Wsh mice+saline, n = 6); and Kit W-sh/W-sh /LPS group (Kit W-sh/Wsh mice+LPS, n = 6).…”

Section: Drug Administrationmentioning

confidence: 99%

The Mast Cell Is an Early Activator of Lipopolysaccharide-Induced Neuroinflammation and Blood-Brain Barrier Dysfunction in the Hippocampus

Wang

Sha

Zhou

et al. 2020

Mediators of Inflammation

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Neuroinflammation contributes to or even causes central nervous system (CNS) diseases, and its regulation is thus crucial for brain disorders. Mast cells (MCs) and microglia, two resident immune cells in the brain, together with astrocytes, play critical roles in the progression of neuroinflammation-related diseases. MCs have been demonstrated as one of the fastest responders, and they release prestored and newly synthesized mediators including histamine, β-tryptase, and heparin. However, temporal changes in MC activation in this inflammation process remain unclear. This study demonstrated that MC activation began at 2 h and peaked at 4 h after lipopolysaccharide (LPS) administration. The number of activated MCs remained elevated until 24 h after LPS administration. In addition, the levels of histamine and β-tryptase in the hippocampus markedly and rapidly increased within 6 h and remained higher than the baseline level within 24 h after LPS challenge. Furthermore, mast cell-deficient KitW-sh/W-sh mice were used to investigate the effects of MCs on microglial and astrocytic activation and blood-brain barrier (BBB) permeability at 4 h after LPS stimulation. Notably, LPS-induced proinflammatory cytokine secretion, microglial activation, and BBB damage were inhibited in KitW-sh/W-sh mice. However, no detectable astrocytic changes were found in WT and KitW-sh/W-sh mice at 4 h after LPS stimulation. Our findings indicate that MC activation precedes CNS inflammation and suggest that MCs are among the earliest participants in the neuroinflammation-initiating events.

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Estimation of body surface area in the musk shrew (Suncus murinus): a small animal for testing chemotherapy-induced emesis

Cited by 4 publications

References 15 publications

Body surface area-based equivalent dose calculation in tree shrew

Body surface area-based equivalent dose calculation in tree shrew

Dosage selection and effect evaluation of sodium pentobarbital in tree shrew anesthesia

The Mast Cell Is an Early Activator of Lipopolysaccharide-Induced Neuroinflammation and Blood-Brain Barrier Dysfunction in the Hippocampus

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