Body surface area-based equivalent dose calculation in tree shrew

Abstract

Tree shrew (Tupaia belangeri) is a promising experimental animal in biomedical research, but the equivalent doses of drugs between tree shrew and human and other animals has not been explored, which hinders its further application in a wider scope. The main objective of this article is to provide a method of equivalent dose conversion between tree shrews and other species based on body surface area (BSA). BSA of tree shrews were measured by Image J software, and then the average Km value of tree shrews was figured out based on the body weights and BSA, then the conversion coefficients of equivalent dose among tree shrew and other species of experimental animals were calculated based known data. The Km value of tree shrews was 0.105 ± 0.001. Through BSA conversion, the equivalent dose for tree shrews (D-ts) relative to rats was obtained by formula: D-ts = 1.36 × D-a (rats weighing 200 g as example), and the error was less than 10% when the BW of the tree shrew was 0.09 kg–0.15 kg. The coefficients of equivalent dose transferring from tree shrews to human and other species were calculated in article. These parameters could be used to determine a suitable dosing strategy for tree shrew studies.

Dosage selection and effect evaluation of sodium pentobarbital in tree shrew anesthesia

“…11,12 In our previous study, we calculated the equivalent dose between tree shrew and rat using the body surface area (BSA) conversion. 13 Therefore, in the current study, we verified the validity of the equivalent dose of sodium pentobarbital for tree shrews. In addition, we compared the most frequently reported dose of sodium pentobarbital with the equivalent dose.…”

“…In group D-BSA (62 mg/kg), tree shrews were anesthetized with a dose of 62 mg/kg, which was converted from the anesthetic dose used in rats (40-50 mg/kg [14][15][16][17] ). Taking the median dose of 45 mg/kg and the conversion coefficient between tree shrews and rats as 1.368, 13 the dosage calculated based on the BSA conversion was 45 mg/kg  1.368 ¼ 61.56 mg/kg % 62 mg/kg). In group D-Rev (40 mg/kg), tree shrews were anesthetized with the most frequently reported dose (40 mg/kg).…”

Effects of total glucosides of paeony on serum inflammatory cytokines in animal models of rheumatoid arthritis: a systematic review and meta-analysis

“…Specifically, in rats (200 g), 0 < low dose (LD) group ≤81.5 mg·kg-1·day-1, 81.5 mg·kg-1·day-1< medium dose (MD) group ≤163 mg·kg-1·day-1, 163 mg·kg-1·day-1 < high dose (HD) group ≤326 mg·kg-1·day-1; in mice (20 g), 0< LD ≤ 117 mg·kg-1·day-1, 117 mg·kg-1·day-1 < MD ≤ 234 mg·kg-1·day-1, 234 mg·kg-1·day-1 < HD ≤ 468 mg·kg-1·day-1. Although the equivalent doses for 200 g rats and 20 g mice obtained by conversion from the standard dose for a 70 kg human are acceptable within a certain body weight range, large conversion errors occur when the actual body weights of the rats or mice are outside that range ( Xia et al, 2021 ). Therefore, in the dose subgroup analysis, we convert the equivalent dose and adjust the low, medium, and high dose ranges one by one according to the mean of the actual body weight of the animals in each study.…”

An Investigation of the Mechanisms of Radiation-Induced Muscle Injury in a Tree Shrew (Tupaia belangeri) Model

“…Before receiving radiation therapy, the tree shrews in all three groups were anesthetized via intraperitoneal injection of 1% pentobarbital sodium (65 mg/kg). 22 Each tree shrew in the radiation-induced chronic and acute injury groups was placed in the left lateral recumbent position with the right ear facing upwards. The right TVP muscle region was irradiated once with a 9 MeV electron beam produced by a linear particle accelerator (Varian Clinac iX, SN4948, software version 9.01; Varian Medical Systems, California, USA) using the following parameters: total radiation dose: 20 Gy, radiation field: 2 cm × 5 cm, source-to-skin distance: 100 cm, depth: 1.9 cm.…”