Estimation of age based on tooth cementum annulations: A comparative study using light, polarized, and phase contrast microscopy

Kaur, Prabhpreet; Astekar, Madhusudan; Singh, Jappreet; Arora, Karandeep Singh; Bhalla, Gagandeep

doi:10.4103/0975-1475.172441

Cited by 24 publications

(20 citation statements)

References 26 publications

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“…It should be noted that, despite the current literature shows the existence of advanced technologies such as three-dimensional (3D) records of palatal roughness 18 , microscopic analysis of incremental lines of dental cement 19 and the use of softwares for morphometric measurements of dental pulps 20 , and a careful forensic dental examination associated with previous data in dental records, are sufficient to contemplate, with precision and rapidity, the success of the identification procedures, without need of further tests for human identification 13 .…”

Section: Resultsmentioning

confidence: 99%

Identifying missing people: the contribution of forensic dentistry and DNA

Corradi

Travassos

Coste

et al. 2017

Rev. odontol. UNESP

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ResumoIntrodução: A identificação humana é uma das principais etapas referentes às questões relacionadas à pessoa desaparecida. Objetivo: O presente estudo visou avaliar a efetividade da identificação humana pelas perícias odontolegais realizadas no Serviço de Antropologia Forense de Belo Horizonte-Minas Gerais, comparando-as com as realizadas por DNA. Metodologia: Estudo transversal, utilizando dados secundários registrados no arquivo do Setor de Antropologia Forense, considerando o período de 2008 a 2014, referente às perícias de identificação odontolegais e DNA. Resultado: Analisando-se apenas as perícias de interesse deste estudo (241), a análise de DNA foi o método utilizado para identificação em 79,3% dos casos e exame odontolegal, 20,7%. Quanto ao tipo de material biológico encaminhado para análise, os cadáveres desconhecidos totalizaram 131 (53,9%), as ossadas 109 (44,9%) e segmentos corporais 3 (1,2%). A análise do tempo efetivamente despendido para a conclusão dos exames mostrou que os realizados pela Odontologia foram mais rápidos que os de DNA. No exame odontolegal, o tempo gasto independe do tipo de documentação odontológica avaliada. Conclusão: A análise dos resultados do presente estudo mostrou que a identificação humana pela técnica odontolegal é eficaz, mais ágil e menos onerosa, contribuindo para uma maior celeridade na resolução de questões envolvidas na localização de pessoas desaparecidas. Descritores: Antropologia forense; odontologia legal; genética forense; identificação biométrica. AbstractIntroduction: Human identification is considered one of the major steps concerning missing people. The Forensic Anthropology Sector of Legal Medical Institutes identifies corpses. Forensic dentistry and DNA tests stand out among the existing standard tests. Objective: This article aimed to evaluate human identification effectiveness through forensic dental examination performed in the forensic anthropology sector in a Forensic Medical Institute, comparing them with DNA analyses. Methodology: This is a cross-sectional study using secondary data available in the department´s database, from 2008 to 2014, concerning identification procedures using forensic dentistry and DNA techniques. Result: The analysis of the examinations eligible to this study (241) showed that DNA analysis was the method used for identification in 79.3% of the cases and forensic dental examinations were used in 20.7% of the cases. As for the type of biological material used during these examinations, unidentified corpses corresponded to 131 cases (53.9%), skeleton structures corresponded to 109 cases (44.9%) and there were 3 cases of body segments (1.2%). When analyzing the time spent to complete the tests, dental examinations were faster than DNA tests. The time spent for forensic dental examination does not depend on the type of dental documentation evaluated. Conclusion: The analysis of the results in this study showed that human identification through forensic dentistry is effective, rapid and less costly, contributing to greater ...

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Section: Resultsmentioning

confidence: 99%

Identifying missing people: the contribution of forensic dentistry and DNA

Corradi

Travassos

Coste

et al. 2017

Rev. odontol. UNESP

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“…determined that incremental lines of cementum were most clearly visible under a phase-contrast microscope and subsequently under polarized microscope and light microscope. [11] Analysts determined the obserever error and tooth variability in this technique for age estimation and concluded that premolars are the preferred tooth with least interobserver error. Also, by increasing the number of slides per tooth, tooth variance can be minimized.…”

Section: Discussionmentioning

confidence: 99%

Age estimation by cemental annulation rings

Mohan

Gokulraj

Thomas

2018

J Forensic Dent Sci

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Context:Tooth plays a pivotal role in the identification of a person when all other remains are ruined by natural or unnatural causes. Dental evidence can contribute to age estimation in forensic dentistry. Estimating the age of an individual with the help of a tooth for identification proves beneficial. Alternating light and dark bands seen on the cementum have been shown to be proportionate to the age of the patient in number. This was done to evaluate the cementum annulation in age determination.Aims:This study aimed to determine the age of an individual using tooth cementum annulation.Materials and Methods:In this study, twenty extracted teeth were collected and all the clinical details of the patient such as age, sex, and chief complaint were recorded. The collected teeth was preserved in formalin overnight and then washed under tap water before they were sectioned. The area selected for counting was observed under 10 × objective of light microscope, and photomicrographs were taken for the counting of the lines. At the time of analysis, the age of the patient was not disclosed to the observer.Statistical Analysis Used:Student's t-test was used for statistical analysis.Results:This study showed the correlation between the actual age of the individual and the estimated age using tooth cementum annulation method and showed significant statistical value.Conclusion:Incremental lines found in cementum can be used for age estimation in forensic dentistry.

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“…Thus, the inference of dental age has high reliability and unique significance in individual recognition (Someda et al, 2009;Aboshi et al, 2010;Tardivo et al, 2011). With age, secondary dentin and reparative dentin continue to deposit, so many scholars select secondary dentin deposition as an age-related research indicator, but this study can only be carried out on the corpse (Bommannavar & Kulkarni, 2015;Chopra et al, 2015;Kaur, et al, 2015;Klumb et al, 2016). To make up for this limitation, on the basis of previous studies, we used oral and craniofacial CT to take three-dimensional (3D) images of maxillary canines, selected the ratio of pulp volume (PV) to tooth volume (TV) (PV/ TV) as an indicator to infer individual age, and deduced the equation of related function for individual age inference by using 3D method so as to provide theoretical guidance for the follow-up clinical work of forensic medicine.…”

Section: Introductionmentioning

confidence: 98%

Morphology and Volume of Maxillary Canine Pulp Cavity for Individual Age Estimation in Forensic Dentistry

Hong-Shangguan

et al. 2017

Int. J. Morphol.

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LI, B.; XIU-PING, W.; HONG-SHANGGUAN; WU, X. & KE-MING, Y.Morphology and volume of maxillary canine pulp cavity for individual age estimation in forensic dentistry. Int. J. Morphol., 35(3):1058Morphol., 35(3): -1062Morphol., 35(3): , 2017. SUMMARY:Age estimation plays a very important role in individual recognition in forensic dentistry. Morphological data of 3D pulp cavity of maxillary canines were acquired by oral and craniofacial CT scans. In accordance with inclusion criteria, CT data of 103 patients (51 males and 52 females) were collected at the Department of Radiology, Stomatological Hospital, Shanxi Medical University, China from September 2015 to August 2016. Mimics 17.0 software was used to measure pulp volume of maxillary canines and tooth volume, and to calculate the ratio of pulp volume/tooth volume. SPSS 16.0 software was utilized to analyze and establish database. Linear regression analysis was applied to process data and to establish a linear regression equation for individual age: Y=69.137-621.200 (pulp volume/tooth volume), R=0.544. Subjects were grouped according to gender, deriving the inferred function of male age: Y=64.333-468.811 (pulp volume/tooth volume), R=0.435; the inferred function of female age: Y=76.445-843.186 (pulp volume/tooth volume), R=0.691. The ratio of pulp volume to tooth volume can be used to infer individual age, and can provide a new method and pathway for individual recognition in forensic dentistry.

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Estimation of age based on tooth cementum annulations: A comparative study using light, polarized, and phase contrast microscopy

Cited by 24 publications

References 26 publications

Identifying missing people: the contribution of forensic dentistry and DNA

Identifying missing people: the contribution of forensic dentistry and DNA

Age estimation by cemental annulation rings

Morphology and Volume of Maxillary Canine Pulp Cavity for Individual Age Estimation in Forensic Dentistry

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