Estimation of age at death based on aspartic acid racemization in noncollagenous bone proteins

Ritz, S.; Turzynski, Andreas; Schütz, H. W.

doi:10.1016/0379-0738(94)90251-8

Cited by 46 publications

(28 citation statements)

References 38 publications

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“…Aspartic acid racemisation therefore served as a marker for the extent of helical integrity in the individual CNBr-fragments. Standard hydrolysis conditions for age estimation in a forensic context, of 6 h at 100°C, were chosen to minimize the induced racemisation of aspartic acid during hydrolysis (Ritz et al 1993(Ritz et al , 1994(Ritz et al , 1996. During preparative hydrolysis asparagine undergoes rapid irreversible deamination to aspartic acid (Hill 1965).…”

Section: Crude Molecular Weight Separation By Gel Filtrationmentioning

confidence: 99%

Archaeological collagen: Why worry about collagen diagenesis?

Dobberstein

Collins

Craig

et al. 2009

Archaeol Anthropol Sci

128

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Section: Crude Molecular Weight Separation By Gel Filtrationmentioning

confidence: 99%

Archaeological collagen: Why worry about collagen diagenesis?

Dobberstein

Collins

Craig

et al. 2009

Archaeol Anthropol Sci

128

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“…[49][50][51] Agedependent amino acid racemization also has been observed in proteins found in vertebral discs and the eye lens. [52][53][54][55] In theory, the racemization process is not directly coupled to the aging of the individual, but is simply a process that depends on when proteins were first generated in the body.…”

Section: Chemical and Molecular Biological Methodsmentioning

confidence: 99%

Age estimation of skeletal remains: principal methods

Villa¹,

Lynnerup²

2014

RRFMS

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Abstract:The age of an individual is often a fundamental piece of data in connection with forensic identification of unidentified bodies. The methods most often used are based on visually determining various morphological, age-related changes in the skeleton (or teeth, although odontological methods are not reviewed in this paper). As such, these methods are all relative: ie, they do not obtain results in calendar years but estimates of the age at death, often with a rather large range. Recently, methods have been proposed for more direct ascertainment of age at death: eg, protein racemization and radiocarbon methods. The latter method, especially, may yield absolute age (year of birth), because radiocarbon activity (as measured in specific proteins in specific cells or tissues of the body) may be in equilibrium with the so-called bomb-pulse, when atmospheric radiocarbon content has changed markedly from one year to another. This review covers the basic and most often used gross morphological methods, radiologically-based methods, biochemical methods, and radiocarbon dating.

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“…Moreover, racemization of aspartic acid has been studied in other parts of human tissues for age estimation in forensic science like bones. The organic matrix of bone composes of about 90% collagen protein and 10% noncollagenous protein like the component in dentin (Ritz et al 1994;Schulz & Jundt 1989). Bone has more metabolic turnover rate of protein than teeth because it growth, modeling and remodeling.…”

Section: Application Of Amino Acid Racemizationmentioning

confidence: 99%

“…Third, aspartic acid racemization in both bone and permanent protein can be an estimator for protein age and organism age, as well. Thus, the relationship between extent of aspartic acid racemization from bone can be used one of indicator for age determination in forensic context (Ritz et al 1994). The previous study examined aspartic acid racemization in human bone both sexes.…”

Section: Application Of Amino Acid Racemizationmentioning

confidence: 99%

“…Furthermore, the bone is affected by diseases (Clarke 2008;Ritz et al 1996). Ritz et al (1994) studied aspartic acid racemization in non-collagenous protein of human bone. They used 35 skulls to racemization analysis and found very close correlation between extent of aspartic acid racemization and age in osteocalcin protein (r = 0.99).…”

Section: Application Of Amino Acid Racemizationmentioning

confidence: 99%

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