Animal populations are regulated by multiple factors, e.g. resource availability, diseases and predators. However, these factors can be significantly influenced by anthropogenic effects (e.g. biological invasions, mesopredator release) and either subserve populations or push them towards local extinction. The Hungarian meadow viper Vipera ursinii rakosiensis is an endangered reptile, with only a few surviving populations following recent alterations and fragmentation of their habitats. In Hungary, all documented populations are located within nature conservation areas. Significant practical conservation efforts have been made, including habitat reconstruction and development, as well as ex situ breeding and reintroductions. Nevertheless, the estimated overall population size of Hungarian meadow viper remains very low, and the impact of conservation interventions is practically immeasurable according to low densities. It is hypothesized that predation is the main factor interfering with conservation efforts of the vipers. Here, we analysed the diet of two mammalian predators (European badger and red fox) in a Hungarian meadow viper habitat. We found a high prevalence of viper remains in the processed faecal samples, indicating that a high number of individuals vipers are subject to predation even within a short period of time. We conclude that effective predator control might be necessary to increase the impact of standard conservation measures (e.g. habitat development, ex situ breeding and reintroduction) that target the restoration of Hungarian meadow viper populations.