Estimating the carbohydrate content of various forms of tobacco by phenol-sulfuric acid method

Jain, Vardhaman Mulchand; Karibasappa, Gundabaktha Nagappa; Dodamani, Arun Suresh; Mali, Gaurao Vasant

doi:10.4103/jehp.jehp_41_17

Cited by 60 publications

(39 citation statements)

References 24 publications

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“…The contents of carbohydrate in crude PCPP and its purified fraction (PCPP-1a) were evaluated by the phenol-sulfuric acid assay using a series of concentrations of D-glucose as standards [31]. The content of sulfate radical was assessed using the reported way [32].…”

Section: Methodsmentioning

confidence: 99%

Enzymatic Extraction, Purification, and Characterization of Polysaccharides fromPenthorum chinense Pursh: Natural Antioxidant and Anti-Inflammatory

Lin

Zhao

Deng

et al. 2018

BioMed Research International

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Penthorum chinense Pursh (PCP) is a kind of functional food or medicine for liver protection. In the present work, Plackett-Burman design, steepest ascent method, and response surface methodology (RSM) were employed to obtain maximum total sugar yield. The experimental yield of 6.91% indicated a close agreement with the predicted yield of 7.00% of the model under optimized conditions. The major polysaccharide fraction (PCPP-1a) from PCPP was purified and identified as acidic polysaccharides with a high content of uronic acid (FT-IR, UV, HPGPC). PCPP had similar monosaccharide profile with PCPP-1a but was rich in galacturonic acid (HPLC). Both of PCPP and PCPP-1a possessed strong hydroxyl radical scavenging, DPPH radical scavenging, and Fe2+ chelating activities. Moreover, they were revealed to show strong anti-inflammatory activities by inhibiting NO, TNF-α, and IL-1β release compared to LPS treatment in RAW264.7 cells. These data suggest that the polysaccharides from PCP could be potential natural products for treating ROS and inflammatory-related diseases.

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Section: Methodsmentioning

confidence: 99%

Enzymatic Extraction, Purification, and Characterization of Polysaccharides fromPenthorum chinense Pursh: Natural Antioxidant and Anti-Inflammatory

Lin

Zhao

Deng

et al. 2018

BioMed Research International

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“…Phenol sulfuric acid assay was done by adding 1 mg/ mL of purified samples with 5% aqueous phenol and 2 mL of concentrated sulphuric acid, kept undisturbed for about 10 minutes, followed by vortex, left at room temperature and the solution was now measured between 200 nm and 800 nm. 30 TGA (thermogravimetric analysis) was performed for purified polysaccharides.…”

Section: Characterization Of the Polysaccharidesmentioning

confidence: 99%

Extraction, Purification, and Characterization of Polysaccharides of Araucaria heterophylla L and Prosopis chilensis L and Utilization of Polysaccharides in Nanocarrier Synthesis

Samrot¹,

Kudaiyappan²,

Bisyarah³

et al. 2020

IJN

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Background: Plant gums consist of polysaccharides which can be used in the preparation of nanocarriers and provide a wide application in pharmaceutical applications including as drug delivery agents and the matrices for drug release. The objectives of the study were to collect plant gums from Araucaria heterophylla L and Prosopis chilensis L and to extract and characterize their polysaccharides. Then to utilize these plant gum-derived polysaccharides for the formulation of nanocarriers to use for drug loading and to examine their purpose in drug delivery in vitro. Methods: Plant gum was collected, polysaccharide was extracted, purified, characterized using UV-Vis, FTIR, TGA and GCMS and subjected to various bioactive studies. The purified polysaccharide was used for making curcumin-loaded nanocarriers using STMP (sodium trimetaphosphate). Bioactivities were performed on the crude, purified and drugloaded nanocarriers. These polysaccharide-based nanocarriers were characterized using UV-Vis spectrophotometer, FTIR, SEM, and AFM. Drug release kinetics were performed for the drug-loaded nanocarriers. Results: The presence of glucose, xylose and sucrose was studied from the UV-Vis and GCMS analysis. Purified polysaccharides of both the plants showed antioxidant activity and also antibacterial activity against Bacillus sp. Purified polysaccharides were used for nanocarrier synthesis, where the size and shape of the nanocarriers were studied using SEM analysis and AFM analysis. The size of the drug-loaded nanocarriers was found to be around 200 nm. The curcumin-loaded nanocarriers were releasing curcumin slow and steady. Conclusion: The extracted pure polysaccharide of A. heterophylla and P. chilensis acted as good antioxidants and showed antibacterial activity against Bacillus sp. These polysaccharides were fabricated into curcumin-loaded nanocarriers whose size was below 200 nm. Both the drug-loaded nanocarriers synthesized using A. heterophylla and P. chilensis showed antibacterial activity with a steady drug release profile. Hence, these natural exudates can serve as biodegradable nanocarriers in drug delivery.

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“…6. Total carbohydrate (mg⋅g -1 DW) was determined as described in [11]. The experiment was designed in a randomized complete block design with three replicates, and three plants for each.…”

Section: Methodsmentioning

confidence: 99%

Influence of salinity on vegetative growth and photosynthetic pigments of bitter almond rootstock

Badran

Махмуд²,

Savin

et al. 2019

Vestn. Ross. univ. družby nar., Ser. Agron. životnovod.

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Bitter almond rootstock is considered one of the most vital rootstocks for stone fruit species but it is classified as a plant sensitive to salinity. This experiment was carried out to study the effect of salt stress on vegetative growth and photosynthetic pigments of bitter almond rootstock as an attempt to sustain growth and increase its tolerance to high salt concentrations. However, the seeds were soaked in salt solution of NaCl as 1, 3, and 5 dsm -1 for 48 hours before stratification. After that, nuts were sown in perlite and treated with different saline solutions subsequently stratified at 6 ℃ for eight weeks. Sprouted seeds were cultivated in pots with a mixture of peat and perlite and treated only with the highest salt concentration 5 dsm -1 . The treatments were arranged in a complete randomized block design with three replications. Vegetative traits and photosynthetic pigments content were estimated. The results revealed that soaking and pre-treating seed of bitter almond rootstock by means of high salt concentration 5dsm -1 during the germination period and subsequently after planting produced stronger transplants that had hardening, adaptation and could avoid the hyperosmotic shock of salt stress after planting. It is obvious throughout; increment of stem diameter, plant height, total number of leaves\plant, fresh and dry weight of leaves, photosynthetic pigments and total carbohydrate content of such transplants. While other coming seedlings from low salt concentrations were exposed to hyperosmotic shock and salt injury therefore inhibit growth rate of such plants, increased falling of leaves and finally reduced photosynthetic pigments content in the resulting seedlings. For citation:Badran AM, Savin IY. Influence of salinity on vegetative growth and photosynthetic pigments of bitter almond rootstock.

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Estimating the carbohydrate content of various forms of tobacco by phenol-sulfuric acid method

Cited by 60 publications

References 24 publications

Enzymatic Extraction, Purification, and Characterization of Polysaccharides fromPenthorum chinense Pursh: Natural Antioxidant and Anti-Inflammatory

Enzymatic Extraction, Purification, and Characterization of Polysaccharides fromPenthorum chinense Pursh: Natural Antioxidant and Anti-Inflammatory

<p>Extraction, Purification, and Characterization of Polysaccharides of <em>Araucaria heterophylla</em> L and <em>Prosopis chilensis</em> L and Utilization of Polysaccharides in Nanocarrier Synthesis</p>

Influence of salinity on vegetative growth and photosynthetic pigments of bitter almond rootstock

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