Estimating the binding ability of collared peccary (Pecari tajacu Linnaeus, 1758) sperm using heterologous substrates

Campos, Lívia Batista; Peixoto, Gislayne Christianne Xavier; Silva, Alexandre Rodrigues; Souza, Ana Liza Paz; Castelo, Thibério de Souza; Maia, Keilla Moreira; Pereira, Alexsandra Fernandes

doi:10.1016/j.theriogenology.2017.01.008

Cited by 21 publications

(17 citation statements)

References 49 publications

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“…Spermatozoa bind to the oocyte ZP and undergo the acrosome reaction during fertilization, which ultimately completes sperm‐egg binding (Campos et al., 2017). Briefly, semen samples were incubated with capacitation medium for 3 hr.…”

Section: Methodsmentioning

confidence: 99%

Notoginsenoside R1 protects boar sperm during liquid storage at 17°C

Ren

Feng

Niu

et al. 2020

Reprod Domestic Animals

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Reactive oxygen species (ROS) damage mammalian sperm during liquid storage. Notoginsenoside R1 (NR1) is a compound isolated from the roots of Panax notoginseng; it has powerful ROS‐scavenging activities. This work hypothesized that the antioxidant capacity of NR1 could improve boar sperm quality and fertility during liquid storage. During liquid storage at 17°C, the supplementation of semen extender with NR1 (50 μM) significantly improved sperm motility, membrane integrity and acrosome integrity after 5 days of preservation. NR1 treatment also reduced ROS and lipid peroxidation (LPO) levels at day 5 (p <0.05). Higher glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) levels and sperm–zona pellucida binding capacity were observed in the 50 μM NR1 group than those in the control group at day 7 (p <0.05). Importantly, statistical analysis of the fertility of 200 sows indicated that addition of NR1 to the extender improved the fertility parameters of boar spermatozoa during liquid storage at 17°C (p <0.05). These results demonstrate the practical feasibility of using 50 μM NR1 as an antioxidant in boar extender during liquid storage at 17°C, which is beneficial to both spermatozoa quality and fertility.

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Section: Methodsmentioning

confidence: 99%

Notoginsenoside R1 protects boar sperm during liquid storage at 17°C

Ren

Feng

Niu

et al. 2020

Reprod Domestic Animals

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“…To increase, the efficiency of coupling, specific molecular binders can be used. There are several biomolecules that can be used to bind the sperm cells to the inner tube surface [64][65][66][67][68][69][70][71][72]. In order to functionalize the inner surface of the microtubes for better entrapment of sperm cells, two attachment methods are utilized-surface linker chemistry and microcontact printing technology.…”

Section: Creating Biohybrid and Synthetic Spermbots-technical Challenmentioning

confidence: 99%

“…To further enhance the performance, binding the sperm cells to the hollow space of microtubes using Fibronectin (Fn) protein and adding caffeine to the environment to boost the motility of the cells was proposed. As discussed above, there are several biomolecules which can be used to bind the sperm cells to the inner tube surface [64][65][66][67][68][69][70][71][72]. Because the microtube is ferromagnetic, its orientation can be controlled and maintained using an external magnetic field-like a compass needle.…”

Section: Spermbot Assisted Targeted Delivery-proof Of Concept Examplementioning

confidence: 99%

Sperm Cell Driven Microrobots—Emerging Opportunities and Challenges for Biologically Inspired Robotic Design

et al. 2020

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With the advent of small-scale robotics, several exciting new applications like Targeted Drug Delivery, single cell manipulation and so forth, are being discussed. However, some challenges remain to be overcome before any such technology becomes medically usable; among which propulsion and biocompatibility are the main challenges. Propulsion at micro-scale where the Reynolds number is very low is difficult. To overcome this, nature has developed flagella which have evolved over millions of years to work as a micromotor. Among the microscopic cells that exhibit this mode of propulsion, sperm cells are considered to be fast paced. Here, we give a brief review of the state-of-the-art of Spermbots—a new class of microrobots created by coupling sperm cells to mechanical loads. Spermbots utilize the flagellar movement of the sperm cells for propulsion and as such do not require any toxic fuel in their environment. They are also naturally biocompatible and show considerable speed of motion thereby giving us an option to overcome the two challenges of propulsion and biocompatibility. The coupling mechanisms of physical load to the sperm cells are discussed along with the advantages and challenges associated with the spermbot. A few most promising applications of spermbots are also discussed in detail. A brief discussion of the future outlook of this extremely promising category of microrobots is given at the end.

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“…The sperm vigor was evaluated using a scale of 0 to 5, with 0 and 5 characterized by no movement and progressive forward movement, respectively. Brome-phenol blue-stained smears were prepared with 5 µL of semen for evaluating sperm viability and morphology using light microscopy (×1000), counting 100 cells (CAMPOS et al, 2017). Finally, the functional integrity of the sperm membrane was evaluated by its osmotic response to a hyposmotic swelling test, using distilled water (0 mOsm/L) as the hyposmotic solution, counting 200 cells per slide (SANTOS et al, 2013).…”

Section: Semen Collection and Evaluationmentioning

confidence: 99%

Superoxide dismutase and catalase activity in collared peccary (Pecari tajacu) seminal plasma and their relation to sperm quality

Santos

Sousa

Silva

et al. 2018

SCA

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The study of the seminal plasma help us to understand the mechanisms by which reactive oxygen species (ROS) affect the sperm. The antioxidant enzymes, as the superoxide dismutase - SOD and catalase - CAT, are capable of removing the oxidative agents before they produce injuries. The aim of the current study was to investigate the activity of antioxidant enzymes SOD and CAT in seminal plasma, and their association with sperm quality in collared peccaries. Study was conducted during the dry period (August and September) on a region characterized by a semiarid climate, with an average annual temperature of 27°C and irregular rainfall (Mossoro, RN, Brazil; 5°10´S and 37°10´W). Nine ejaculates were obtained from sexually mature males (1 sample per animal) by electroejaculation. Semen was evaluated for microscopic parameters and the activity of SOD and CAT was measured by spectrophotometry. All ejaculates were white in color. Mean values for concentration were of 207 ± 160 x106 sperm/mL, motility of 83.0 ± 20.9% and viability of 72.5 ± 10.4%. In regards to the enzymatic activity, none was observed for the CAT enzyme. Trace levels of SOD (0.034 ± 0.049 AU/mgP) were detected in the ejaculates of all individuals; however, no correlation was observed between SOD levels and the sperm motility (R = 0.35; P = 0.931), vigor (R = 0.29; P = 0.133), viability (R = 0.16; P = 0.29), functional membrane (R = 0.04; P = 0.617) or morphology (R = 0.03; P = 0.637). In conclusion, we demonstrated the first description of antioxidant enzyme activity in seminal plasma of fresh ejaculates obtained from collared peccaries. SOD antioxidant activity was evident during the dry period of a semi-arid region, but no relationship between SOD and semen parameters was observed.

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Estimating the binding ability of collared peccary (Pecari tajacu Linnaeus, 1758) sperm using heterologous substrates

Cited by 21 publications

References 49 publications

Notoginsenoside R1 protects boar sperm during liquid storage at 17°C

Notoginsenoside R1 protects boar sperm during liquid storage at 17°C

Sperm Cell Driven Microrobots—Emerging Opportunities and Challenges for Biologically Inspired Robotic Design

Superoxide dismutase and catalase activity in collared peccary (Pecari tajacu) seminal plasma and their relation to sperm quality

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