The influence of sacrificial anode electrochemical properties interfered by direct stray current (DC) of 0 V, 1 V, 3 V, and 5 V, with different chloride ion concentration and temperature, was studied by open circuit potential (OCP), electrochemical impedance spectroscopy (EIS), and polarization curves. The specific performance was as follows: as the DC interference voltage increased from 0 V to 5 V, the degree of positive migration of the sacrificial anode open circuit potential increased. The effect of temperature in DC interference voltage environment on sacrificial anode corrosion was not great, but the low temperature of 10 ∘ C could slow down the sacrificial anode corrosion and maintain good work efficiency. With the increase of the ambient temperature, the degree of corrosion of the sacrificial anode was deepened. As the chloride ion concentration in DC interference voltage environment increased from 0% to 0.3%, the degree of positive migration of the sacrificial anode open circuit potential increased. The higher the chloride ion concentration was, the greater the impact on the performance of the sacrificial anode was.