Esterification of fatty acids by <i>Penicillium crustosum</i> lipase in a membrane reactor

Possebom, Gessica; Nyari, Nádia Ligianara Dewes; Zeni, Jamile; Steffens, Juliana; Rigo, Elisandra; Luccio, Marco Di

doi:10.1002/jsfa.6630

Cited by 5 publications

(4 citation statements)

References 31 publications

(36 reference statements)

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“…Although the remaining activity yielded decent conversion in our case, it poses a problem for its wider application as a cofactor recycling system. Other enzymes, such as glucose dehydrogenases [28], could be used for the recycling of the cofactor but complexity of the system is increased with the added substrate and product generated. To surpass this limitation, the use of other formate dehydrogenases, which pose the advantage of limited residues and excellent atom economy, capable to better withstand covalent immobilisation could greatly benefit the proposed system.…”

Section: Discussionmentioning

confidence: 99%

“…In this case, instead of covalently immobilising the enzyme, which normally causes a loss of activity due to increased rigidification, the enzymatic cocktail is placed inside a dialysis membrane. This allows the free flow of the substrate and product in and out of the membrane but avoids the loss of enzyme to the reaction bulk, allowing its reuse in subsequent reactions, even in continuous using membrane reactors [27,28].…”

Section: Introductionmentioning

confidence: 99%

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Efficient Amino Donor Recycling in Amination Reactions: Development of a New Alanine Dehydrogenase in Continuous Flow and Dialysis Membrane Reactors

2021

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Transaminases have arisen as one of the main biocatalysts for amine production but despite their many advantages, their stability is still a concern for widespread application. One of the reasons for their instability is the need to use an excess of the amino donor when trying to synthesise amines with unfavourable equilibria. To circumvent this, recycling systems for the amino donor, such as amino acid dehydrogenases or aldolases, have proved useful to push the equilibria while avoiding high amino donor concentrations. In this work, we report the use of a new alanine dehydrogenase from the halotolerant bacteria Halomonas elongata which exhibits excellent stability to different cosolvents, combined with the well characterised CbFDH as a recycling system of L-alanine for the amination of three model substrates with unfavourable equilibria. In a step forward, the amino donor recycling system has been co-immobilised and used in flow with success as well as re-used as a dialysis enclosed system for the amination of an aromatic aldehyde.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Efficient Amino Donor Recycling in Amination Reactions: Development of a New Alanine Dehydrogenase in Continuous Flow and Dialysis Membrane Reactors

2021

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“…Many studies have demonstrated that enzyme recycling by using membranes is an easy and feasible technique (Agustian et al 2011;Marques et al 2017;Nguyen et al 2014;Possebom et al 2014). Membranes with specific pore sizes are selected to retain or separate free or immobilized enzyme in the production medium.…”

Section: Introductionmentioning

confidence: 99%

Dialysis membrane enclosed laccase catalysis combines a controlled conversion rate and recyclability without enzyme immobilization

Zhang

Wang

et al. 2020

AMB Expr

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Laccase is a versatile multicopper oxidase that holds great promise for many biotechnological applications. For such applications, it is essential to explore good biocatalytic systems for high activity and recyclability. The feasibility of membrane enclosed enzymatic catalysis (MEEC) for enzyme recycling with laccase was evaluated. The dialysis membrane enclosed laccase catalysis (DMELC) was tested for the conversion of the non-phenolic model substrate 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonate) (ABTS). Trametes versicolor laccase was found to be completely retained by the dialysis membrane during the process. The ABTS total conversion after DMELC reached the same values as the batch reaction of the enzyme in solution. The efficiency of DMELC conversion of ABTS under different process conditions including shaking speed, temperature, ABTS concentration and pH was investigated. The repetitive dialysis minimally affected the activity and the protein content of the enclosed laccase. DMELC retained 70.3 ± 0.8% of its initial conversion after 5 cycles. The usefulness of MEEC extends to other enzymes with the benefit of superior activity of an enzyme in solution and the recyclability which is normally only obtained with immobilized enzymes.

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“…According to the reaction type, polymeric, inorganic, ceramic, metallic materials can be used as membrane and these are simply characterized as porous or dense membrane [7][8][9][10]. Esterification, wastewater treatment, oxidation and hydrogen production are well known commercial CMR applications [11][12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous separation performance of a catalytic membrane reactor for ethyl lactate production by using boric acid coated carboxymethyl cellulose membrane

Nigiz

Hilmioğlu

2016

Reac Kinet Mech Cat

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In this study, a boric acid coated carboxymethyl cellulose composite catalytic membrane was prepared and employed in a pervaporation assisted membrane reactor system to synthesize ethyl lactate. The effect of catalyst loading, temperature, initial molar feed ratio and reaction run time were investigated to evaluate reaction and separation ability of the hybrid pervaporation catalytic membrane reactor (PVCMR) system. The reaction performance was obtained by using lactic acid conversion data. Separation performance was determined as functions of flux and selectivity. It was observed that the conversion increased from 51 to 71 % as the temperature increased from 55 to 75°C. The best result was obtained as 83 % at 75°C when initial alcohol:acid molar ratio was three. Also, it was seen that the catalytic membrane preserved 93.6 % of its activity. The system efficiency was evaluated by comparison of PVCMR data to simply batch reactor data under the same conditions.

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Esterification of fatty acids by Penicillium crustosum lipase in a membrane reactor

Abstract: The confinement of lipase from P. crustosum in a membrane reactor was possible, resulting in the satisfactory conversion of butyric acid to ethyl butyrate with the possibility of reuse of the immobilized enzyme.

Cited by 5 publications

References 31 publications

Efficient Amino Donor Recycling in Amination Reactions: Development of a New Alanine Dehydrogenase in Continuous Flow and Dialysis Membrane Reactors

Efficient Amino Donor Recycling in Amination Reactions: Development of a New Alanine Dehydrogenase in Continuous Flow and Dialysis Membrane Reactors

Dialysis membrane enclosed laccase catalysis combines a controlled conversion rate and recyclability without enzyme immobilization

Simultaneous separation performance of a catalytic membrane reactor for ethyl lactate production by using boric acid coated carboxymethyl cellulose membrane

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