Dialysis membrane enclosed laccase catalysis combines a controlled conversion rate and recyclability without enzyme immobilization

Abstract

Laccase is a versatile multicopper oxidase that holds great promise for many biotechnological applications. For such applications, it is essential to explore good biocatalytic systems for high activity and recyclability. The feasibility of membrane enclosed enzymatic catalysis (MEEC) for enzyme recycling with laccase was evaluated. The dialysis membrane enclosed laccase catalysis (DMELC) was tested for the conversion of the non-phenolic model substrate 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonate) (ABTS). Trametes versicolor laccase was found to be completely retained by the dialysis membrane during the process. The ABTS total conversion after DMELC reached the same values as the batch reaction of the enzyme in solution. The efficiency of DMELC conversion of ABTS under different process conditions including shaking speed, temperature, ABTS concentration and pH was investigated. The repetitive dialysis minimally affected the activity and the protein content of the enclosed laccase. DMELC retained 70.3 ± 0.8% of its initial conversion after 5 cycles. The usefulness of MEEC extends to other enzymes with the benefit of superior activity of an enzyme in solution and the recyclability which is normally only obtained with immobilized enzymes.

Electric‐field‐enhanced selective separation of products of an enzymatic reaction in a membrane micro‐contactor

“…Another study focused on cellulase recycling by centrifugation was reported in (Xin et al, 2020). Free laccase enveloped by a dialysis membrane was repeatedly used for the conversion of 2,2′‐azino‐bis(3‐ethylbenzthiazoline‐6‐sulfonate; Zhang et al, 2020). In our group, we successfully recycled PA in the processes of cephalexin synthesis coupled with a product extraction mediated by an aqueous two‐phase system (Vobecká et al, 2020).…”

Extracellular L-Asparaginase Synthesis Bacillus niacin Isolation, Optimization, and Characterization from Marine Saltern Sediment Sources

“…Finally, replace the buffer and dialyze at 4 °C overnight. The dialysis bag sample was then used as a protein source for future research 19 .…”

Efficient Amino Donor Recycling in Amination Reactions: Development of a New Alanine Dehydrogenase in Continuous Flow and Dialysis Membrane Reactors

“…Taking advantage of the better performance of the enzymes in their soluble form and to prevent the inactivation of HeFDH after immobilisation, another strategy was adopted. Very recently, it has been reported that the enclosure of a laccase in a dialysis bag retains the original activity of the enzyme and help its reusability, avoiding protein immobilisation [39].…”