Esterase from a cariogenic bacterium hydrolyzes dental resins

Abstract

The current study builds upon our highly-cited previous study by Bourbia et al., (JDR, 2013) that reported on that the cariogenic bacterium, S. mutans has esterase-like activities that enable the bacterium to degrade dental composites and adhesives. The current submission is the first to report on the isolation and characterization of the specific esterase activity (SMU_118c) from S. mutans that is a significant contributor to the whole bacterial degradative activity toward the hydrolysis of dental resins. This activity compromises the restoration-tooth interface, increases interfacial bacterial microleakage (Kermanshahi et al., JDR 2010), potentially contributing to the pathogenesis of recurrent caries around resin composite restorations. This represent a significant contribution to the field of biomaterials and their clinical performance.

Endodontic pathogens possess collagenolytic properties that degrade human dentine collagen matrix

“…, Huang et al . , Marashdeh et al . ) could potentially enable the bacteria to compromise the restoration‐tooth and sealer‐dentine interfaces.…”

“…Enterococcus faecalis has the ability to produce acid and can tolerate low pH (Ramsey et al 2014). Acids produced by sugar fermentation from E. faecalis and/or other bacteria, such as S. mutans (Huang et al 2018) can demineralize dental hard tissues and expose the demineralized dentinal collagen. Once exposed, they become accessible to host and bacterial proteases that can degrade the exposed dentinal collagen.…”

“…Several endodontic pathogens possess collagenase protease activities and are capable of degrading dentinal collagen. In combination with the bacteria's ability to produce acid and ability to hydrolyse methacrylate-based resins (Bourbia et al 2013, Huang et al 2018, Marashdeh et al 2018) could potentially enable the bacteria to compromise the restoration-tooth and sealer-dentine interfaces. Further studies are required to identify and characterize the specific proteolytic activities from the bacteria to allow for the development of anticollagenolytic sealers and/or treatment that could be used to mitigate the effect of the bacteria.…”

Biostable, antidegradative and antimicrobial restorative systems based on host-biomaterials and microbial interactions

“…Human saliva has been shown to contain a variety of hydrolase activities, with the most important in relation to restorative resins being hydrolases and esterases. These have a number of sources, from human gingiva, salivary glands, immune cells, as well as the multitude of bacterial species in the mouth [10, 50, 51], These esterases cleave the vulnerable ester linkages in many common dental resins, accelerating the biodegradation of these materials [49, 52, 53], Esterase activity in human saliva has been characterized as cholesterol esterase-like (CE) and pseudocholinesterase-like (PCE), released in part by normal as well as inflamed gingiva [54, 55], These two esterases show strong ability to degrade bisGMA, TEGDMA, HEMA, and other ester-containing monomers in dental resins.…”

“…S. mutans ’ role in biodegradation was further investigated by Huang et al [51, 61, 79]. In this work, the genome of S. mutans UA159, a clinically-relevant cariogenic strain (that had its genome mapped by Ajdic et al [80]) was searched for potential esterases.…”