Estagiamento de embriões de Macrobrachium olfersi (Wiegman) (Crustacea, Palaemonidae) através de critérios morfológicos nos dias embrionários

Simões-Costa, Marcos; Pacheco, Cristiane; Nazari, Evelise Maria; Müller, Yara Maria Rauh; Ammar, Dib

doi:10.1590/s0101-81752005000200029

Cited by 13 publications

(8 citation statements)

References 22 publications

(20 reference statements)

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“…AbdB and abdA are genes involved in the formation of abdominal segments and are necessary for adequate standardization in the posterior region in P. hawaiensis (Martin et al, 2016;Serano et al, 2016). AbdB and abdA transcripts in M. olfersii embryos increased from E5 to E6 (8.1-and 21.5-fold, respectively; Figure 2i,j), which is in accordance with the growth and curvature of the caudal papilla (stage E6) for abdomen formation (Simões-Costa et al, 2005). These results suggest that Hox genes in M. olfersii are involved in the identity of the body segment, as occurs in other studied crustaceans (Abzhanov & Kaufman, 1999;Martin et al, 2016;Serano et al, 2016).…”

Section: Isoforms Ofsupporting

confidence: 64%

“…A similar temporal expression profile was observed in the Hox cluster genes of the decapod E. sinensis (Chinese mitten crab) from the gastrula stage to before the Zoea I stage (Cui et al, 2021). The formation of the head in M. olfersii starts at E4 before the formation of the posterior region of the embryo (Simões-Costa et al, 2005). Dfd, lab, pb, and Scr genes are expressed in specific segments in the head region of other crustaceans, as in P. scaber (Abzhanov & Kaufman, 1999) and P. hawaiensis (Serano et al, 2016).…”

Section: Isoforms Ofsupporting

confidence: 61%

“…To obtain embryos, adult females and males collected in the Peri Lagoon (27° 35′S, 48° 35′W) were placed in aquarium (60L) at proportion of 3:1 (female: male), maintained at 24°C (±1), with constant aeration under photoperiod conditions (12 h light: 12 h dark) and fed daily with balanced feed (Alcon Bottom Fish). Staging of embryos was performed daily according to Simões‐Costa et al (2005). To obtain mature and immature ovaries, external observation of the ovary's size and color was performed (Mossolin & Bueno, 2002).…”

Section: Methodsmentioning

confidence: 99%

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Identification of Hox genes and their expression profiles during embryonic development of the emerging model organism, Macrobrachium olfersii

et al. 2022

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Hox genes encode transcription factors that specify the body segment identity during development, including crustaceans, such as amphipods and decapods, that possess a remarkable diversity of segments and specialized appendages. In amphipods, alterations of specialized appendages have been obtained using knockout experiment of Hox genes, which suggests that these genes are involved in the evolution of morphology within crustaceans. However, studies of Hox genes in crustaceans have been limited to a few species. Here, we identified the homeodomain of nine Hox genes: labial (lab), proboscipedia (pb), Deformed (Dfd), Sex combs reduced (Scr), fushi tarazu (ftz), Antennapedia (Antp), Ultrabithorax (Ubx), abdominal‐A (abdA), and Abdominal‐B (AbdB), and evaluated their expression by RT‐qPCR and RT‐PCR in the ovary, during embryonic development, and at the first larval stage (Zoea I) of the decapod Macrobrachium olfersii. The transcript levels of lab, Dfd, and ftz decreased and transcripts of pb, Scr, Antp, Ubx, abdA, and AbdB increased during embryonic development. Hox genes were expressed in mature ovaries and Zoea I larval stages, except Scr and ftz, respectively. In addition, isoforms of Dfd, Scr, Ubx, and abdA, which have been scarcely reported in crustaceans, were described. New partial sequences of 87 Hox genes from other crustaceans were identified from the GenBank database. Our results are interesting for future studies to determine the specific function of Hox genes and their isoforms in the freshwater prawn M. olfersii and to contribute to the understanding of the diversity and evolution of body plans and appendages in Crustaceans.

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Section: Isoforms Ofsupporting

confidence: 64%

Section: Isoforms Ofsupporting

confidence: 61%

Section: Methodsmentioning

confidence: 99%

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Identification of Hox genes and their expression profiles during embryonic development of the emerging model organism, Macrobrachium olfersii

et al. 2022

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“…This increase in the eye index of M. acanthurus corroborates findings reported for the crab Erimacrus isenbeckii (Brandt, 1848) (Nagao et al 1999) and for the lobster Homarus americanus Milne edwards, 1837, which showed an increase by 490 μm during incubation (Perkins 1972). An increase in the pigmentation percentage as development proceeds was already observed in palaemonids as M. olfersii (Müller et al 2003;Simões-Costa et al 2005), M. potiuna (Müller et al 2004), M. idella idella (Dinakaran et al 2013) and M. lamarrei (Rashid et al 2013) ( Table 1).…”

Section: Discussionmentioning

confidence: 70%

Effect of salinity on the embryonic development ofMacrobrachium acanthurus(Decapoda: Palaemonidae)

Fukuda

Bertini

Almeida

2016

Invertebrate Reproduction & Development

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Palaemonid shrimps occur in the tropical and temperate regions of South America and the Indo-Pacific, in brack-ish/freshwater habitats, and marine coastal areas. They form a clade that recently (i.e., ~30 mya) invaded freshwater, and one included genus, Macrobrachium Bate, 1868, is especially successful in limnic habitats. Adult Macrobrachium acanthurus (Wiegmann, 1836) dwell in coastal freshwaters, have diadromous habit, and need brackish water to develop. Thus, they are widely recognized as euryhaline. Here we test how this species responds to a short-term exposure to increased salinity. We hypothesized that abrupt exposure to high salinity would result in reduced gill ventilation/perfusion and decreased oxygen consumption. Shrimps were subjected to control (0 psu) and experimental salinities (10, 20, 30 psu), for four and eight hours (n = 8 in each group). The water in the experimental containers was saturated with oxygen before the beginning of the experiment; aeration was interrupted before placing the shrimp in the experimental container. Dissolved oxygen (DO), ammonia concentration, and pH were measured from the aquaria water, at the start and end of each experiment. After exposure, the shrimp's hemolymph was sampled for lactate and osmolality assays. Muscle tissue was sampled for hydration content (Muscle Water Content, MWC). Oxygen consumption was not reduced and hemolymph lactate did not increase

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“…Thus, considering this brief panorama, the aim of this study was to investigate the effect of UVB radiation on embryonic cells of the freshwater prawn Macrobrachium olfersi, which are found in Southern Brazil, a region where high intensities of UV radiation are recorded. It is important to remark that during development, embryos stay for 14 days in the external ventral brood pouch of the ovigerous female . The pouch is open and this facilitates a constant revolving of the eggs toward the center and periphery of the egg mass, allowing all of them to be exposed to virtually equal UVB radiation.…”

Section: Introductionmentioning

confidence: 99%

Light‐mediated DNA Repair Prevents UVB‐induced Cell Cycle Arrest in Embryos of the Crustacean Macrobrachium olfersi

Zeni

Ammar

Leal

et al. 2015

Photochem & Photobiology

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High levels of ultraviolet-B (UVB) radiation can negatively affect aquatic animals. Macrobrachium olfersi is a prawn that lives in clear freshwaters and during the breeding season, females carry eggs in an external brood pouch. Therefore, we hypothesize that eggs are also exposed to environmental UVB radiation. The aim of this study was to investigate whether UVB radiation induces DNA damage and compromises cell cycle in embryos of M. olfersi. In laboratory, UVB irradiance (310 mW. cm(-2) ) that embryos receive in the natural environment was simulated. After irradiation, embryos were kept under different light conditions in order to recognize the presence of cell repair. UVB radiation induces DNA damage, specifically thymine dimers. After 48 h of UVB exposure, a significant decrease in the level of these dimers was observed in embryos kept under visible light while it remained constant in the dark. Moreover, under visible light and darkness, a decrease in proliferation was observed after 48 h of irradiation. An increase in PCNA expression and decrease in p53 expression were observed after, respectively, 1 and 48 h of exposure. Our results showed that UVB radiation disturbs the cell cycle and induces DNA damage in M. olfersi embryos. However, under visible light these embryos showed successful DNA repair.

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Estagiamento de embriões de Macrobrachium olfersi (Wiegman) (Crustacea, Palaemonidae) através de critérios morfológicos nos dias embrionários

Cited by 13 publications

References 22 publications

Identification of Hox genes and their expression profiles during embryonic development of the emerging model organism, Macrobrachium olfersii

Identification of Hox genes and their expression profiles during embryonic development of the emerging model organism, Macrobrachium olfersii

Effect of salinity on the embryonic development ofMacrobrachium acanthurus(Decapoda: Palaemonidae)

Light‐mediated DNA Repair Prevents UVB‐induced Cell Cycle Arrest in Embryos of the Crustacean Macrobrachium olfersi

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