Establishment of Two Cell Lines from Embryonic Cells of Pectinophora gossypiella (Lepidoptera: Gelichiidae)

Léry, Xavier; El-Tarras, Adel; Monsarrat, Annie; Abol-Ela, S.; Giannotti, J.

doi:10.1303/aez.31.557

Cited by 1 publication

(1 citation statement)

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“…The population dou-bling time was calculated at several passages using an exponential formula. Polypeptide profiles were determined after electrophoresis in 12% polyacrylamide gels with a Biorad Miniprotean II dual slab cell (Léry et al, 1996) and the polypeptide profile of all the established cell lines were compared.…”

Section: Characterization Of Cellsmentioning

confidence: 99%

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Léry¹,

El-Tarras²,

Abol-Ela³

et al. 1998

Cytotechnology

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Three selected uncloned Pop 2, Pop 3, Pop 4 and two cloned cell lines Pop cl1A and Pop cl2B were derived from the original cell line established from Phthorimaea operculella (ORS-Pop-93). Three new non-selected cell lines ORS-Pop-94A, ORS-Pop-94B and ORS-Pop-95 were also established from embryos of the same insect. Differences in morphology, growth rate and polypeptide profile were determined between these cell lines. All the cell lines were susceptible to the Autographa californica nucleopolyhedrovirus (AcMNPV). The cloned cell lines produced higher levels of AcMNPV (TCID-50 and PIB) than the parental cells and at the same rate as the Sf9 reference cell line. Substantial amounts of viral DNA were synthesized in the clone Pop cl 2B after infection with the granulosis virus of the potato tuber moth P. operculella (PTMGV) and a complete multiplication was obtained in the ORS-Pop-95 cell line. The comparison between Pop cell lines which support limited or complete replication of certain baculoviruses can offer insights into some of the molecular barriers which restrict the host range of these viruses. These cell lines with variable susceptibility to baculoviruses could also be used for in vitro recombinations, increasing their virus host range to be used for the control of this pest.

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Section: Characterization Of Cellsmentioning

confidence: 99%