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Abstract

Three selected uncloned Pop 2, Pop 3, Pop 4 and two cloned cell lines Pop cl1A and Pop cl2B were derived from the original cell line established from Phthorimaea operculella (ORS-Pop-93). Three new non-selected cell lines ORS-Pop-94A, ORS-Pop-94B and ORS-Pop-95 were also established from embryos of the same insect. Differences in morphology, growth rate and polypeptide profile were determined between these cell lines. All the cell lines were susceptible to the Autographa californica nucleopolyhedrovirus (AcMNPV). The cloned cell lines produced higher levels of AcMNPV (TCID-50 and PIB) than the parental cells and at the same rate as the Sf9 reference cell line. Substantial amounts of viral DNA were synthesized in the clone Pop cl 2B after infection with the granulosis virus of the potato tuber moth P. operculella (PTMGV) and a complete multiplication was obtained in the ORS-Pop-95 cell line. The comparison between Pop cell lines which support limited or complete replication of certain baculoviruses can offer insights into some of the molecular barriers which restrict the host range of these viruses. These cell lines with variable susceptibility to baculoviruses could also be used for in vitro recombinations, increasing their virus host range to be used for the control of this pest.

Structure and Stability of Keplerian Magnetohydrodynamic Jets

“…As with Ferreira (1997), it is possible to show, as it is done in paper I, that the minimum mass loss rate has a lower limit and can not be arbitrarily small. We also agree with Ostriker (Ostriker 1997) and Lery et al (Lery et al 1999b) who conclude that the optical jet may represent only the densest part of the total outflow.…”

“…It is now well known that outflows can be accelerated from an accretion disk ("Disk wind", Blandford & Payne 1982, Pelletier & Pudritz 1992, Fiege & Henriksen 1996, Contopoulos & Lovelace 1994, Ferreira & Pelletier 1993, Ferreira 1997, Vlahakis & Tsinganos 1998, Lery et al Lery et al 1999b), or at the disk-magnetosphere boundary ("X-winds", Shu et al 1988Shu et al , 1994. Therefore, a Keplerian rotation profile is one of the most realistic description for rotation of sources producing jets.…”

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MHD jet equilibria that depend on source properties are obtained using a simplified model for stationary, axisymmetric and rotating magnetized outflows (Lery et al Lery et al 1998,Lery et al 1999a). The present rotation laws are more complex than previously considered and include a Keplerian disc.

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“…where p is the slope of the solution of the Bernoulli equation at the Alfvèn point. The reader should see Heyvaerts & Norman 1989, Lery et al 1998and Lery et al 1999a for a more detailed explanation of the the equations and methods 2. The projection of the equation of motion on B P yields by integration the Bernoulli equation, E(a) = v 2 /2 + (γ/(γ − 1)) Qρ γ−1 + G(r, z) − rΩB θ /µ 0 α.…”

The Propagation of Magnetocentrifugally Launched Jets. I.

“…Using these equations we model the propagation of a magnetized jet through a constant density, constant pressure magnetized ambient medium. The initial conditions for the jet, i.e., its cross sectional distribution of ρ, p, v and B, are calculated via the Given Geometry Method of (Lery et al 1998, Lery & Frank 1999. We describe this method and the equilibrium MHD jet solutions it produces in the next section.…”