Establishment of Tolerance to Both Cadmium and Copper Stress by Expressing Arabidopsis Phytochelatin Synthase in Cu Tolerant Yeast Mutant

Selection and Isolation of a Mutant Yeast Strain Tolerant to Multiple Targeted Heavy Metals

“…These results suggest that the increased tolerance to one metal results in increased sensitivity to other metal(s). Consistent with this finding, a previous study showed that heterologous expression of a Cd-resistance gene in yeast leads to increased tolerance to Cd with a concomitantly increased sensitivity to Cu (Lee and Kim, 2010). CdR1-CuR7-NiR8 cells showed higher tolerance to Ni, as demonstrated by slightly inhibited growth at 5 mM Ni, whereas the growth of control cells was almost inhibited at that Ni concentration (Fig.…”

“…Yeast cells were grown in yeast nitrogen base supplemented with appropriate amino acids and 2% glucose without uracil (Lee and Kim, 2010). A Cd-tolerant mutant (designated CdR1) was isolated by growing yeast cells in media containing CdCl2 concentrations that were gradually increased up to 1 mM CdCl2.…”

Cadmium stress tolerance in crop plants

“…The expression of Arabidopsis PCS in the yeast mutant deficient in PCS resulted in Cd tolerance but sensitive to Cu which suggests that PCS cannot be used in bioremediation of Cd in Cu contaminated sites. 141 …”

Cadmium tolerance increased synergistically by heterologous co-expression of SpHMT1 and AtPCS1 in budding yeast

“…Recent reports also indicated that SpHMT1 is involved in the sequestration of GSH-Cd complexes into the vacuole [Lee, 2009;Prévéral et al, 2009]. Moreover, overexpression of SpHMT1 confers Cd tolerance to either PCS-or HMT1-deficient fission yeast cells [Prévéral et al, 2009, Lee andKim, 2010]. Therefore, the exact relationship between PCS and HMT1 in the Cd detoxification mechanism is unclear.…”

“…Arabidopsis thaliana MT cDNA was isolated from a *Corresponding author Phone: +82-53-950-7345; Fax: +82-53-953-7233 E-mail: sangman@knu.ac.kr http://dx.doi.org/10. The A. thaliana PC synthase (AtPCS1) cDNA was subcloned into BamHI/SacI sites of the pYES2 vector (Invitrogen), resulting in pYES2:AtPCS1 [Lee and Kim, 2010]. The A. thaliana PC synthase (AtPCS1) cDNA was subcloned into BamHI/SacI sites of the pYES2 vector (Invitrogen), resulting in pYES2:AtPCS1 [Lee and Kim, 2010].…”