Establishment of the Brown Citrus Aphid (Homoptera: Aphididae) in Central America and the Caribbean Basin and Transmission of Citrus Tristeza Virus

Yokomi, R. K.; Lastra, R.; Stoetzel, M. B.; Damsteegt, V. D.; Lee, R. F.; Garnsey, S. M.; Gottwald, T. R.; Rocha-Peña, M. A.; Niblett, C. L.

doi:10.1093/jee/87.4.1078

Cited by 117 publications

(81 citation statements)

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“…Although Toxoptera citricida Kirkaldy is the most efficient CTV aphid vector species (Yokomi et al, 1994), Aphis gossypii Glover is the main CTV vector in the absence of T. citricida (Cambra et al, 2000a;Yokomi & DeBorde, 2005).…”

Section: Introductionmentioning

confidence: 99%

Epidemiology of Citrus tristeza virus in nursery blocks of Citrus macrophylla and evaluation of control measures

Vidal

Moreno

Bertolini

et al. 2012

Span. j. agric. res.

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The control of Citrus tristeza virus (CTV), the causal agent of tristeza disease, is essential to guarantee a productive citrus industry. The specific features of a nursery block make the control of the natural spread of viruses transmitted by vectors more difficult. Thus, the knowledge of the epidemiology of CTV in nursery blocks is basic to the design control strategies of the disease. Two experimental nursery plots of alemow (Citrus macrophylla) were planted in open field in Moncada (Valencia, Spain) (with a high CTV prevalence, 85%), and in Alcanar (Tarragona, Spain) with 700 and 843 nursery plants, respectively. The plants were analysed by tissue print-ELISA using 3DF1 and 3CA5 CTVspecific monoclonal antibodies. The CTV prevalence estimated after one year of cultivation in both plots was of 24.37% and 3.91%, respectively. At the same time, a commercial nursery plot grown under plastic-net covers established in Alcanar with 1,200 plants was also analysed as above, being the CTV prevalence estimated of 0.00%. The aphid activity present in the nursery plots was monitored. Aphis spiraecola was the most abundant aphid species visiting the plants in the nursery plots grown in the open field, whereas the aphid activity registered in the plants grown under the plasticnet covers was practically zero. The percentage of individual aphids carrying CTV PCR-amplifiable targets detected by squash real-time RT-PCR in the open-field nursery plots was 17.5% in Moncada and 1.67% in Alcanar. No significant differences in the CTV prevalence between treated and non-treated plants with horticultural mineral oils were found in the area with the high CTV-inoculum pressure.Additional key words: alemow; horticultural mineral oil treatments; physical barriers; semi-persistent virus; squash real-time RT-PCR; tissue print-ELISA; vector intensity. Resumen Epidemiología del virus de la tristeza de los cítricos en bloques de vivero de Citrus macrophylla y evaluación de medidas de controlEl control del virus de la tristeza de los cítricos (Citrus tristeza virus, CTV), agente causal de la enfermedad de la tristeza, es esencial para garantizar una industria citrícola productiva. Las características específicas de los viveros hacen más difícil el control de los virus de vegetales transmitidos por insectos vectores. Así, el conocimiento de la epidemiología de CTV en bloques de plantas de vivero es básico para diseñar estrategias de control de la enfermedad. Dos parcelas experimentales de vivero cultivadas en campo abierto de Citrus macrophylla se establecieron en Moncada (Valencia, España) (con una alta prevalencia de CTV, 85%) y Alcanar (Tarragona, España) con 700 y 843 plantas de vivero, respectivamente. Las plantas se analizaron mediante Inmunoimpresión-ELISA usando los anticuerpos monoclonales específicos 3DF1 y 3CA5 de CTV. La prevalencia de CTV estimada en ambas parcelas después de un año de cultivo fue del 24,37% y del 3,91%, respectivamente. Al mismo tiempo, una parcela de vivero comercial cultivada bajo cubierta de plástico con...

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Section: Introductionmentioning

confidence: 99%

Epidemiology of Citrus tristeza virus in nursery blocks of Citrus macrophylla and evaluation of control measures

Vidal

Moreno

Bertolini

et al. 2012

Span. j. agric. res.

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“…They vary in transmission efficiency, host range, and distribution (Rocha-Pena et al 1995). The brown citrus aphid is the most efficient vector of CTv (Yokomi et al 1994). This aphid is common in some citrus growing regions but it is still absent in Turkey and other countries in the mediterranean Basin except for a small region in the north of Portugal and Spain.…”

Section: Introductionmentioning

confidence: 99%

Detection ofCitrus tristeza virus(CTV) from Satsuma Owari mandarins(Citris unshiu)by direct tissue blot immunoassay (DTBIA), DAS‐ELISA, and biological indexing

Korkmaz¹,

Çevik

Önder

et al. 2008

New Zealand Journal of Crop and Horticultural Science

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were indexed for biological properties. The results obtained from DTBIA tests showed that 20 of 119 (16.8%) tested trees were infected with CTV and 99 trees were virus free. All samples that tested positive in DTBIA were also positive in DAS-ELISA. After biological indexing, none of these 20 isolates showed any symptoms on sour orange, grapefruit, and sweet orange plants, but all isolates induced vein-clearing symptoms on Mexican lime (Citrus aurantifoli) in 9 months. Some isolates also caused leaf-cupping and chlorosis in Mexican lime. However, 15 months after initial grafting, all isolates induced mild to moderate stem-pitting on Mexican lime. In addition, young tissues, petioles, and leaf samples were collected periodically at monthly intervals for 1 year (2006) from a 25-year-old Satsuma Owari mandarin grafted on P. trifoliata in the Edremit Gulf. CTV was readily detected in tissue blots of young shoots and petioles from CTV-infected plants during the autumn, winter, and spring seasons. Similarly, the highest ELISA values were obtained in April; the lowest values were noted in September. This study showed that DTBIA is a rapid, sensitive, and reliable procedure for detecting CTV under field conditions.

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“…Compared to other plant diseases, citrus tristeza disease has received little attention in Brazil in recent years, despite its enormous historical importance and the economic threat that it still represents to other countries (Lee et al, 1992;Yokomi et al, 1994;Gottwald et al, 1994Gottwald et al, , 1998Rocha-Peña et al, 1995;Hughes and Gottwald, 2001;Riac, 2001;Hughes et al, 2002;Stover and Castle, 2002). To a large extent, this lack of interest reflects the success of agricultural research in this country in dealing with this disease since the late 1940s (Franco and Bacchi, 1944;Meneghini, 1946), but is also related to the characteristics and mode of transmission of the disease (Bitancourt and Rodrigues Filho, 1948;Bennett and Costa, 1949;Bitancourt, 1954).…”

Section: Introductionmentioning

confidence: 99%

The genetics of tolerance to tristeza disease in citrus rootstocks

et al. 2004

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Controlled pollinations between four elite citrus rootstocks, Citrus limonia -'Limeira' rangpur lime (Cravo), C. sunki -'Sunki' mandarin (Sunki), C. aurantium -'São Paulo' sour orange (Azeda) and Poncirus trifoliata -'Davis A' trifoliate orange (Trifoliata), resulted in 1614 nucelar and 1938 hybrid plants identified by the isozyme loci Pgi-1, Pgm-1, Got-1, Got-2, Aps-1, Me-1, Prxa-1 and or by the morphological markers broadness of leaf petiole wing or trifoliolate leaves. Tolerance to the citrus tristeza virus (CTV) was evaluated under nursery and field conditions for several years by the reaction of Valencia orange infected with a severe strain of CTV and grafted onto the hybrids and nucellar clones. Genetic analyses indicated that tolerance was controlled by at least two loci designated here as Az and t interacting in dominant-recessive epistasis. Genotypes Az__ __ __ and __ __ tt were tolerant while azaz T__ was intolerant. The intolerant Azeda was azaz TT, the tolerant rootstocks Sunki and Cravo were Azaz tt and the Trifoliata was Azaz TT. The different degrees of intolerance seen in some hybrids may reflect the inability of segregating modifiers from parental clones to overcome the epistatic interaction that controls the major tolerance reaction.

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Establishment of the Brown Citrus Aphid (Homoptera: Aphididae) in Central America and the Caribbean Basin and Transmission of Citrus Tristeza Virus

Cited by 117 publications

References 0 publications

Epidemiology of Citrus tristeza virus in nursery blocks of Citrus macrophylla and evaluation of control measures

Epidemiology of Citrus tristeza virus in nursery blocks of Citrus macrophylla and evaluation of control measures

Detection ofCitrus tristeza virus(CTV) from Satsuma Owari mandarins(Citris unshiu)by direct tissue blot immunoassay (DTBIA), DAS‐ELISA, and biological indexing

The genetics of tolerance to tristeza disease in citrus rootstocks

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