Establishment of successively transplantable rabbit VX2 cancer cells that express enhanced green fluorescent protein

Oshiro, Hisashi; Fukumura, Hidenobu; Nagahama, Kiyotaka; Sato, Itaru; Sugiura, Kei; Iobe, Hiroaki; Okiyama, Emi; Nagao, Toshitaka; Nagashima, Yoji; Aoki, Ichiro; Yamanaka, Shōji; Murakami, Ayumi; Maegawa, Jiro; Chishima, Takashi; Ichikawa, Yasushi; Ishikawa, Yoshihiro; Nagai, Takeshi; Nomura, Masatoshi; Ohashi, Kenichi; Okihara, Koji

doi:10.1007/s00795-014-0071-2

Cited by 5 publications

(7 citation statements)

References 30 publications

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“…The isolated VX2 tumor cells were further cultured for 5 days, and the morphology of cells was recorded to compare it with that in the literature. The VX2 tumor cells were spindle-to-stellate-shaped cells that could be mono-or multi-nucleated, showing shapes similar to those in the literature (Supporting Figure S2) [37]. The confocal microscope images of the VX2 tumor cells showed that the NIRF signal of the Cy5.5-CNPs in the cytoplasm was gradually increased by the treatment concentration of Cy5.5-CNPs at 24 h (Figure 2a).…”

Section: Cellular Uptake and Cytotoxicity Of Cnpssupporting

confidence: 75%

Tumor-Targeting Glycol Chitosan Nanoparticles for Image-Guided Surgery of Rabbit Orthotopic VX2 Lung Cancer

Rho

Yoon

et al. 2020

Pharmaceutics

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Theranostic nanoparticles can deliver therapeutic agents as well as diverse imaging agents to tumors. The enhanced permeation and retention (EPR) effect is regarded as a crucial mechanism for the tumor-targeted delivery of nanoparticles. Although a large number of studies of the EPR effect of theranostic nanoparticles have been performed, the effect of the change in the body size of the host on the EPR effect is not fully understood. In this regard, comparative research is needed on the behavior of nanoparticles in large animals for developing the nanoparticles to the clinical stage. In this study, we prepared fluorophore (indocyanine green (ICG) or cyanine 5.5 (Cy5.5))–conjugated glycol chitosan nanoparticles (CNPs) for comparing the tumor-targeting efficacy in VX2 tumor-bearing mouse and rabbit models. As expected, the CNPs formed nano-sized spherical nanoparticles and were stable for 8 days under aqueous conditions. The CNPs also exhibited dose-dependent cellular uptake into VX2 tumor cells without cytotoxicity. The half-life of the near-infrared fluorescence (NIRF) signals in the blood were 3.25 h and 4.73 h when the CNPs were injected into mice and rabbits, respectively. Importantly, the CNPs showed excellent tumor accumulation and prolonged biodistribution profiles in both the VX2 tumor-bearing mouse and rabbit models, wherein the tumor accumulation was maximized at 48 h and 72 h, respectively. Based on the excellent tumor accumulation of the CNPs, finally, the CNPs were used in the image-guided surgery of the rabbit orthotopic VX2 lung tumor model. The lung tumor tissue was successfully removed based on the NIRF signal from the CNPs in the tumor tissue. This study shows that CNPs can be potentially used for tumor theragnosis in small animals and large animals.

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Section: Cellular Uptake and Cytotoxicity Of Cnpssupporting

confidence: 75%

Tumor-Targeting Glycol Chitosan Nanoparticles for Image-Guided Surgery of Rabbit Orthotopic VX2 Lung Cancer

Rho

Yoon

et al. 2020

Pharmaceutics

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“…Rabbit squamous cell carcinoma (VX2) cells were purchased from American Type Culture Collection (ATCC) (Virginia, U.S.A.). VX2 cells were transfected with an EGFP-encoding vector as previously described 23 . Human oral squamous cell carcinoma cell lines, OSC-19 and HSC-3, were purchased from the Health Science Research Resources Bank (Japan Health Sciences Foundation, Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

“…Male Japanese white rabbits weighing 2.0–2.5 kg were purchased from Nihon SLC (Shizuoka, Japan). Under general anesthesia, we implanted VX2 cells transfected with an Enhanced Green Fluorescent Protein (EGFP)-encoding vector in the right edge of the tongue to create a rabbit tongue cancer model 23 28 29 , in which to examine the effect of Fe(Salen), the electromagnet, and AMF. The tumors were allowed to grow to a size of 7–10 mm in length (usually for 7 days) prior to treatment.…”

Section: Methodsmentioning

confidence: 99%

Simultaneous hyperthermia-chemotherapy with controlled drug delivery using single-drug nanoparticles

Sato

Umemura

Mitsudo

et al. 2016

Sci Rep

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We previously investigated the utility of μ-oxo N,N′- bis(salicylidene)ethylenediamine iron (Fe(Salen)) nanoparticles as a new anti-cancer agent for magnet-guided delivery with anti-cancer activity. Fe(Salen) nanoparticles should rapidly heat up in an alternating magnetic field (AMF), and we hypothesized that these single-drug nanoparticles would be effective for combined hyperthermia-chemotherapy. Conventional hyperthermic particles are usually made of iron oxide, and thus cannot exhibit anti-cancer activity in the absence of an AMF. We found that Fe(Salen) nanoparticles induced apoptosis in cultured cancer cells, and that AMF exposure enhanced the apoptotic effect. Therefore, we evaluated the combined three-fold strategy, i.e., chemotherapy with Fe(Salen) nanoparticles, magnetically guided delivery of the nanoparticles to the tumor, and AMF-induced heating of the nanoparticles to induce local hyperthermia, in a rabbit model of tongue cancer. Intravenous administration of Fe(Salen) nanoparticles per se inhibited tumor growth before the other two modalities were applied. This inhibition was enhanced when a magnet was used to accumulate Fe(Salen) nanoparticles at the tongue. When an AMF was further applied (magnet-guided chemotherapy plus hyperthermia), the tumor masses were dramatically reduced. These results indicate that our strategy of combined hyperthermia-chemotherapy using Fe(Salen) nanoparticles specifically delivered with magnetic guidance represents a powerful new approach for cancer treatment.

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“…[ 27 ] A group in Japan has developed a VX2 cell line that expresses GFP, and was able to propagate the line in rabbit hind limbs. [ 28 ] Given that cell infusion can lead to peritoneal seeding, our technique would be useful with this animal model, as less peritoneal seeding is seen, thereby, leaving little peritoneal contamination of the peritoneal cavity with GFP.…”

Section: Discussionmentioning

confidence: 99%

Percutaneous Ultrasound Guided Implantation of VX2 for Creation of a Rabbit Hepatic Tumor Model

et al. 2015

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Creation of a VX2 tumor model has traditionally required a laparotomy and surgical implantation of tumor fragments. Open surgical procedures are invasive and require long procedure times and recovery that can result in post-operative morbidity and mortality. The purpose of this study is to report the results of a percutaneous ultrasound guided method for creation of a VX2 model in rabbit livers. A total of 27 New Zealand white rabbits underwent a percutaneous ultrasound guided approach, where a VX2 tumor fragment was implanted in the liver. Magnetic resonance imaging was used to assess for tumor growth and necropsy was performed to determine rates of tract seeding and metastatic disease. Ultrasound guided tumor implantation was successful in all 27 rabbits. One rabbit died 2 days following the implantation procedure. Two rabbits had no tumors seen on follow-up imaging. Therefore, tumor development was seen in 24/26 (92%) rabbits. During the follow-up period, tract seeding was seen in 8% of rabbits and 38% had extra-hepatic metastatic disease. Therefore, percutaneous ultrasound guided tumor implantation safely provides reliable tumor growth for establishing hepatic VX2 tumors in a rabbit model with decreased rates of tract seeding, compared to previously reported methods.

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Establishment of successively transplantable rabbit VX2 cancer cells that express enhanced green fluorescent protein

Cited by 5 publications

References 30 publications

Tumor-Targeting Glycol Chitosan Nanoparticles for Image-Guided Surgery of Rabbit Orthotopic VX2 Lung Cancer

Tumor-Targeting Glycol Chitosan Nanoparticles for Image-Guided Surgery of Rabbit Orthotopic VX2 Lung Cancer

Simultaneous hyperthermia-chemotherapy with controlled drug delivery using single-drug nanoparticles

Percutaneous Ultrasound Guided Implantation of VX2 for Creation of a Rabbit Hepatic Tumor Model

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