Establishment of Stable Chinese Hamster Ovary Cell Line Capable of Expressing Human Recombinant Hemopexin: A Promising Therapeutic Modality Against Hemolytic Anemia

Bahadori, Marzie; Amiri, Fatemeh; Movahed, Marjan; Roushandeh, Amaneh Mohammadi; Roudkenar, Mehryar Habibi

doi:10.5812/gct.13360

Cited by 3 publications

(1 citation statement)

References 38 publications

(51 reference statements)

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“…The most prominent mammalian host cell lines for recombinant mAb expression include the chinese hamster ovary (CHO), mouse myeloma derived NS0 and Sp2/0 cells, human embryonic kidney cells (HEK293), and human embryonic retinoblast-derived PER.C6 cells ( Beck et al, 2008 ). CHO cells are believed to be the most used “workhorse” for today’s industrially produced recombinant products ( Bahadori et al, 2017 ). One of the approaches toward obtaining enhanced production efficiency and improved quality of antibodies from mammalian cell lines is through engineering of the host cell ( Wells and Robinson, 2017 ).…”

Section: Cell Engineering Toward Production Of Novel Antibodies For Tmentioning

confidence: 99%

Cell Line Techniques and Gene Editing Tools for Antibody Production: A Review

Dangi

Sinha²,

Dwivedi

et al. 2018

Front. Pharmacol.

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The present day modern formulation practices for drugs are based on newer tools and techniques toward effective utilization. The methods of antibody formulations are to be revolutionized based on techniques of cell engineering and gene editing. In the present review, we have discussed innovations in cell engineering toward production of novel antibodies for therapeutic applications. Moreover, this review deciphers the use of RNAi, ribozyme engineering, CRISPR-Cas-based techniques for better strategies for antibody production. Overall, this review describes the multidisciplinary aspects of the production of therapeutic proteins that has gained more attention due to its increasing demand.

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Section: Cell Engineering Toward Production Of Novel Antibodies For Tmentioning

confidence: 99%

Cell Line Techniques and Gene Editing Tools for Antibody Production: A Review

Dangi

Sinha²,

Dwivedi

et al. 2018

Front. Pharmacol.

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Bioengineering for Production of Biologically Active Compounds in Plants

Singh

2024

Biosynthesis of Natural Products in Plants

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A Bench-Top Approach for Isolation of Single Antibody Producing Chinese Hamster Ovary (CHO) Cells Using a Microwell-Based Microfluidic Device

et al. 2022

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Genetically-modified monoclonal cell lines are currently used for monoclonal antibody (mAbs) production and drug development. The isolation of single transformed cells is the main hindrance in the generation of monoclonal lines. Although the conventional limiting dilution method is time-consuming, laborious, and skill-intensive, high-end approaches such as fluorescence-activated cell sorting (FACS) are less accessible to general laboratories. Here, we report a bench-top approach for isolating single Chinese hamster ovary (CHO) cells using an adapted version of a simple microwell-based microfluidic (MBM) device previously reported by our group. After loading the cell suspension to the device, the electrostatically trapped cells can be viewed under a microscope and transferred using a micropipette for further clone establishment. Compared to the conventional method, the invented approach provided a 4.7-fold increase in the number of single cells isolated per round of cell loading and demonstrated a 1.9-fold decrease in total performing time. Additionally, the percentage of correct single-cell identifications was significantly improved, especially in novice testers, suggesting a reduced skill barrier in performing the task. This novel approach could serve as a simple, affordable, efficient, and less skill-intensive alternative to the conventional single-cell isolation for monoclonal cell line establishment.

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Establishment of Stable Chinese Hamster Ovary Cell Line Capable of Expressing Human Recombinant Hemopexin: A Promising Therapeutic Modality Against Hemolytic Anemia

Abstract: Background: Hemolytic anemia is associated with intravascular heme release and oxidative stresses that lead to endothelial dys-

Cited by 3 publications

References 38 publications

Cell Line Techniques and Gene Editing Tools for Antibody Production: A Review

Cell Line Techniques and Gene Editing Tools for Antibody Production: A Review

Bioengineering for Production of Biologically Active Compounds in Plants

A Bench-Top Approach for Isolation of Single Antibody Producing Chinese Hamster Ovary (CHO) Cells Using a Microwell-Based Microfluidic Device

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