Establishment of PCR conditions for determination of <i>Silybum marianum</i> genetic diversity using ISSR marker

Jamshidnia, M.; Asgary, S.; Rafieian-Kopaei, M.

doi:10.17660/actahortic.2020.1297.52

Cited by 2 publications

(1 citation statement)

References 18 publications

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“…The PCR was carried out using a thermo cycler (ABI, PCR System 2080, Perkin-Elmer Corp, Norwalk, CT, USA). The following cycling protocol was set for ampli cation: 2 min initial denaturation at 94°C; 10 cycles of 2 min denaturation at 94°C, 2 min annealing at 50-60°C, and 30 s extension at 72°C; 25 cycles of 30 s denaturation at 94°C, 1 min annealing at 50°C, and 1 min extension at 72°C; and a nal extension step of 10 min at 72°C[46,47]. Ampli cation products were stored at -4°C.…”

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confidence: 99%

Analysis of phytoplasma-infected Chinese cherry (Prunus pseudocerasus Lindl.) based on intersimple sequence repeat (ISSR) and methylation sensitive amplification polymorphism (MSAP)

Cai

Chen

et al. 2023

Preprint

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Background Chinese cherry (Prunus pseudocerasus Lindl.) is a fruit crop that is susceptible to phytoplasma infection, which causes symptoms such as virescence, phyllody, sterility and stiff fruit. To investigate the effects of phytoplasma infection on the genome and DNA methylation of Chinese cherry, we performed inter-simple sequence repeat (ISSR) and methylation-sensitive amplified polymorphism (MSAP) analyses on the leaves and floral organs of healthy and infected plants from Qijiang District of Chongqing. Results ISSR analysis revealed no significant differences in the genomic DNA of leaves and floral organs between healthy and infected plants, suggesting that phytoplasma infection did not induce genomic mutations. MSAP analysis showed that phytoplasma infection caused epigenetic variations in both leaves and floral organs, with different degrees of DNA methylation and demethylation. These epigenetic changes may affect gene expression and lead to abnormal plant development. Conclusions This study provides insights into the molecular mechanisms of Chinese cherry phytoplasma disease and fruit development. Potential candidate genes associated with hard fruit formation were also identified, which may be useful for future research in this area.

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confidence: 99%

Analysis of phytoplasma-infected Chinese cherry (Prunus pseudocerasus Lindl.) based on intersimple sequence repeat (ISSR) and methylation sensitive amplification polymorphism (MSAP)

Cai

Chen

et al. 2023

Preprint

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Evaluation of accession structure and genetic diversity in Iranian milk thistle (Silybum marianum L.) by ISSR markers

Jamshidnia,

Asgary,

Rafieian-Kopaei

2023

Genetika

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The aim of this study was to investigate the genetic variations of Iranian Silybum marianum accessions using inter simple sequence repeat (ISSR) markers. Twenty-nine accessions from various Iran regions and a sample from Hungary were selected and evaluated by 19 ISSR primers. A clear banding pattern was produced by 9 primers and a total of 85 repeatable polymorphic bands were detected. ISSR7 and ISSR8 primers identified the most Polymorphic Information Content (PIC) with 100% among primers. In the tested accessions, polymorphism information content (0.45), polymorphic percentage (83 %), and Shannon?s information index (0.53) assessed a high level of genetic variation. Based on Jaccards distances, cluster analysis molecular traits with unweighted pair group method with arithmetic mean (UPGMA) method were taken into consideration, and accessions were grouped into nine clusters and confirmed by principal coordinate analysis. Four clusters were identified by a Bayesian structure analysis, and 12 individuals were maintained inside the admixed clusters. According to the findings, ISSR marker system can be considered as a powerful tool for detection of genetic diversity of accessions in S. marianum. The results indicate existence of a high variation among Iranian S. marianum accessions to start the breeding programs.

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Establishment of PCR conditions for determination of Silybum marianum genetic diversity using ISSR marker

Cited by 2 publications

References 18 publications

Analysis of phytoplasma-infected Chinese cherry (Prunus pseudocerasus Lindl.) based on intersimple sequence repeat (ISSR) and methylation sensitive amplification polymorphism (MSAP)

Analysis of phytoplasma-infected Chinese cherry (Prunus pseudocerasus Lindl.) based on intersimple sequence repeat (ISSR) and methylation sensitive amplification polymorphism (MSAP)

Evaluation of accession structure and genetic diversity in Iranian milk thistle (Silybum marianum L.) by ISSR markers

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