Establishment of introgression libraries in hybrid rye (Secale cereale L.) from an Iranian primitive accession as a new tool for rye breeding and genomics

Abstract

Genetic diversity of elite breeding material can be increased by introgression of exotic germplasm to ensure long-term selection response. The objective of our study was to develop and characterize the first two rye introgression libraries generated by marker-assisted backcrossing and demonstrate their potential application for improving the baking quality of rye. Starting from a cross between inbred line L2053-N (recurrent parent) and a heterozygous Iranian primitive population Altevogt 14160 (donor) two backcross (BC) and three selfing generations were performed to establish introgression libraries A and B. Amplified fragment length polymorphisms (AFLP markers) and simple sequences repeats (SSRs) were employed to select and characterize candidate introgression lines (pre-ILs) from BC(1) to BC2S3. The two introgression libraries comprise each 40 BC2S3 pre-ILs. For analyzing the phenotypic effects of the exotic donor chromosome segment (DCS) we evaluated the per se performance for pentosan and starch content in replicated field trials at each of four locations in 2005 and 2006. Introgression library A and B cover 74 and 59% of the total donor genome, respectively. The pre-ILs contained mostly two to four homozygous DCS, with a mean length of 12.9 cM (A) and 10.0 cM (B). We detected eight (A) and nine (B) pre-ILs with a significant (P<0.05) higher pentosan content and two pre-ILs (B) with a significant (P<0.05) higher starch content than the elite recurrent parent. Thus, our results indicate that exotic genetic resources in rye carry favorable alleles for baking quality traits, which can be exploited for improving the elite breeding material by marker-assisted selection (MAS). These introgression libraries can substantially foster rye breeding programs and provide a promising opportunity to proceed towards functional genomics.

Genetic dissection of intersubgenomic heterosis in Brassica napus carrying genomic components of B. rapa

“…It is crucial to use the most appropriate related species to complement the lack of elite alleles in some cultivated crops, to increase genetic diversity, and to develop superior crop cultivars (Falke et al 2008;Feuillet et al 2008;Kovach and McCouch 2008;Zamir 2001). The introgression of B. rapa into B. napus has been an effective approach to improve rapeseed (Qian et al 2005;Zou et al 2010).…”

Hybrid breeding boosted molecular genetics in rye

“…Библиотеки покрывают около 70 % всего генома донора. Фенотипи-ческая оценка упомянутой библиотеки интрогрессивных линий ржи уже выявила значительную генетическую изменчивость в отношении количественно наследуемых признаков, таких как хлебопекарное качество и восста-новление фертильности пыльцы (Falke et al, 2008(Falke et al, , 2009). …”

Methylation-sensitive amplified polymorphism-based genome-wide analysis of cytosine methylation profiles in Nicotiana tabacum cultivars

“…Two restriction endonucleases (EcoRI and MseI) are usually used in AFLP to slice the genome in order to form restriction endonuclease-sliced fragments with different ends. The technology has been widely used to analyze the relationships between germplasm resources in rice (Oryza, Chandel et al, 2010), cotton (Gossypium, Zhang et al, 2005), corn (Zea mays L., Frascaroli et al, 2013), barley (Hordeum brevisubulatum, Shan et al, 2012), peanuts (Arachis hypogaea, Jiang et al, 2007), rye (Secale cereale L., Falke et al, 2008), and tobacco (Nicotiana tabacum, Bahulikar et al, 2004). However, few studies have investigated the relationship between epigenetic factors, yield, and quality.…”