Establishment of in vitro culture, plant regeneration, and genetic transformation of Camelina sativa

Yemets, А. І.; Boychuk, Yu. N.; Shysha, E. N.; Rakhmetov, Dzhamal; Blume, Ya. B.

doi:10.3103/s0095452713030031

Cited by 18 publications

(19 citation statements)

References 15 publications

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“…Interest in C. sativa increased in recent years due to its adaptability to diverse environmental conditions, low requirements for water and nutrients, relatively strong resistance to insect pests and microbial diseases, and unique oil composition and characteristics suitable for the production of food and fodder, biofuels, and bio-based products [ 1 , 2 , 7 , 8 ]. These positive agronomic traits and environmental attributes, along with the recent development of methods for transgenesis [ 9 , 10 , 11 , 12 , 13 ] and CRISPR/Cas genome editing [ 14 , 15 , 16 ], triggered great interest in C. sativa as an industrial oilseed crop. The ongoing interest in C. sativa is documented by the large number of peer-reviewed publications from various databases retrieved when “camelina” was used as a search term, for example, in a query of the ScienceDirect (2309 publications from 1997–2021), Web of Science (1525 publications from 2000–2021) and Agricola (677 publications from 2000–2021) databases (reported on December 30, 2021).…”

Section: Introductionmentioning

confidence: 99%

Realizing the Potential of Camelina sativa as a Bioenergy Crop for a Changing Global Climate

Neupane

Lohaus

Solomon

et al. 2022

Plants

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Camelina sativa (L.) Crantz. is an annual oilseed crop within the Brassicaceae family. C. sativa has been grown since as early as 4000 BCE. In recent years, C. sativa received increased attention as a climate-resilient oilseed, seed meal, and biofuel (biodiesel and renewable or green diesel) crop. This renewed interest is reflected in the rapid rise in the number of peer-reviewed publications (>2300) containing “camelina” from 1997 to 2021. An overview of the origins of this ancient crop and its genetic diversity and its yield potential under hot and dry growing conditions is provided. The major biotic barriers that limit C. sativa production are summarized, including weed control, insect pests, and fungal, bacterial, and viral pathogens. Ecosystem services provided by C. sativa are also discussed. The profiles of seed oil and fatty acid composition and the many uses of seed meal and oil are discussed, including food, fodder, fuel, industrial, and medical benefits. Lastly, we outline strategies for improving this important and versatile crop to enhance its production globally in the face of a rapidly changing climate using molecular breeding, rhizosphere microbiota, genetic engineering, and genome editing approaches.

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Section: Introductionmentioning

confidence: 99%

Realizing the Potential of Camelina sativa as a Bioenergy Crop for a Changing Global Climate

Neupane

Lohaus

Solomon

et al. 2022

Plants

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“…Another option is the use of direct genomic modification utilizing biotechnological techniques. Being a close relative of Arabidopsis, there are 30 years of molecular biology techniques for genetic modification available for use [41][42][43]. To date, genome modification has been used to enhance the metabolic pathway for fatty acid production in order to enhance production levels [32,33,[44][45][46][47].…”

Section: Introductionmentioning

confidence: 99%

“…To date the herbicide bar selection system is the only robust selection method available. Others marker genes such as hygromycin (hptII) [43] and acetolactate synthase (ALS) [42,[63][64][65] have been published, but do not appear as robust as bar, and therefore may have limited use as a biotechnological tool.…”

Section: Introductionmentioning

confidence: 99%

Phenotypic Examination of Camelina sativa (L.) Crantz Accessions from the USDA-ARS National Genetics Resource Program

Hotton

Kammerzell

Chan

et al. 2020

Plants

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Camelina sativa (L.) Crntz. is a hardy self-pollinated oilseed plant that belongs to the Brassicaceae family; widely grown throughout the northern hemisphere until the 1940s for production of vegetable oil but was later displaced by higher-yielding rapeseed and sunflower crops. However, interest in camelina as an alternative oil source has been renewed due to its high oil content that is rich in polyunsaturated fatty acids, antioxidants as well as its ability to grow on marginal lands with minimal requirements. For this reason, our group decided to screen the existing (2011) National Genetic Resources Program (NGRP) center collection of camelina for its genetic diversity and provide a phenotypic evaluation of the cultivars available. Properties evaluated include seed and oil traits, developmental and mature morphologies, as well as chromosome content. Selectable marker genes were also evaluated for potential use in biotech manipulation. Data is provided in a raw uncompiled format to allow other researchers to analyze the unbiased information for their own studies. Our evaluation has determined that the NGRP collection has a wide range of genetic potential for both breeding and biotechnological manipulation purposes. Accessions were identified within the NGRP collection that appear to have desirable seed harvest weight (5.06 g/plant) and oil content (44.1%). Other cultivars were identified as having fatty acid characteristics that may be suitable for meal and/or food use, such as low (<2%) erucic acid content, which is often considered for healthy consumption and ranged from a high of 4.79% to a low of 1.83%. Descriptive statistics are provided for a breadth of traits from 41 accessions, as well as raw data, and key seed traits are further explored. Data presented is available for public use.

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“…Different in vitro culture protocols have been tested and evaluated for organogenesis or somatic embryogenesis of Camelina. These protocols have been mainly focused on shoot organogenesis and regeneration using cotyledon with petiole, hypocotyl segment and leaf explants (Yemets et al, 2013), microspore culture (Ferrie and Bethune, 2011), and somatic hybridization (Narasimhulu et al, 1994). It has been confirmed that Camelina can be efficiently transformed by Agrobacterium-mediated floral-dip methods, and a variety of selectable markers (e.g., seed fluorescence and resistance to specific herbicides or antibiotics) can be easily used to identify Camelina transgenic seeds (Liu et al, 2012; Bansal and Durrett, 2016).…”

Section: Tissue Culture Optimization For Camelinamentioning

confidence: 99%

Fueling the future; plant genetic engineering for sustainable biodiesel production

2018

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HIGHLIGHTS Genetic engineering potentials to enhance oil production in emerging nonedible oil plants for economic biodiesel production were discussed. Expression, overexpression, or suppression of major genes affecting oil yield, oil composition, and tolerance to biotic/abiotic stresses in bioenergy crops were reviewed. Major genes widely used to enhance oil content and composition were reviewed. Challenges in commercialization of GM oil plants were presented.

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Establishment of in vitro culture, plant regeneration, and genetic transformation of Camelina sativa

Cited by 18 publications

References 15 publications

Realizing the Potential of Camelina sativa as a Bioenergy Crop for a Changing Global Climate

Realizing the Potential of Camelina sativa as a Bioenergy Crop for a Changing Global Climate

Phenotypic Examination of Camelina sativa (L.) Crantz Accessions from the USDA-ARS National Genetics Resource Program

Fueling the future; plant genetic engineering for sustainable biodiesel production

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