Establishment of human embryonic stem cell lines from frozen-thawed blastocysts using STO cell feeder layers

Park, Se-Pill; Lee, Young Jae; Lee, Keum Sil; Shin, Hyun Ah; Cho, Hwang Yoon; Chung, Kyuha; Kim, Eun Young; Lim, Jin Ho

doi:10.1093/humrep/deh102

Cited by 74 publications

(50 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In fact, there was a first peak of generation of new hESC lines in 2004, contributed mostly from one group in the USA [12], one in Korea [13] and two in Sweden [14,15], which accounted for 16, 9 and 10 of the total of 38 new cell lines derived that year, respectively (Fig. 1).…”

Section: Gathering Information From Human Embryonic Stem Cell Registriesmentioning

confidence: 99%

A Survey of Parameters Involved in the Establishment of New Lines of Human Embryonic Stem Cells

Fraga¹,

Araújo²,

Stabellini

et al. 2011

Stem Cell Rev and Rep

View full text Add to dashboard Cite

Since the derivation of the first human embryonic stem cell (hESC) lines by Thomson and coworkers in 1998, more than 1,200 different hESC lines have been established worldwide. Nevertheless, there is still a recognized interest in the establishment of new lines of hESC, particularly from HLA types and ethnic groups currently underrepresented among the available lines. The methodology of hESC derivation has evolved significantly since 1998, when human LIF (hLIF) was used for maintenance of pluripotency. However, there are a number of different strategies for the several steps involved in establishing a new line of hESC. Here we make a survey of the most relevant parameters used between 1998 and 2010 for the derivation of the 375 hESC lines deposited in two international stem cell registries, and able to form teratomas in immunocompromised mice. Although we identify some trends in the methodology for establishing hESC lines, our data reveal a much greater heterogeneity of strategies than what is used for derivation of murine ESC lines, indicating that optimum conditions have not been consolidated yet, and thus, hESC establishment is still an evolving field of research.

show abstract

Section: Gathering Information From Human Embryonic Stem Cell Registriesmentioning

confidence: 99%

A Survey of Parameters Involved in the Establishment of New Lines of Human Embryonic Stem Cells

Fraga¹,

Araújo²,

Stabellini

et al. 2011

Stem Cell Rev and Rep

View full text Add to dashboard Cite

show abstract

“…The mouse embryonic fibroblast cell line, STO, has been used to establish nine cell lines from frozen blastocysts and zygotes (Park et al, 2004). The advantage of STO cells over primary cultures of MEFs is that, being immortalized, they are easy to maintain and propagate.…”

Section: Alternative Feeder Cellsmentioning

confidence: 99%

Toward xeno-free culture of human embryonic stem cells

Mallon

Park

Chen

et al. 2006

The International Journal of Biochemistry & Cell Biology

158

View full text Add to dashboard Cite

The culture of human embryonic stem cells (hESCs) is limited, both technically and with respect to clinical potential, by the use of mouse embryonic fibroblasts (MEFs) as a feeder layer. The concern over xenogeneic contaminants from the mouse feeder cells may restrict transplantation to humans and the variability in MEFs from batch-to-batch and laboratory-to-laboratory may contribute to some of the variability in experimental results. Finally, use of any feeder layer increases the work load and subsequently limits the large-scale culture of human ES cells. Thus, the development of feeder-free cultures will allow more reproducible culture conditions, facilitate scale-up and potentiate the clinical use of cells differentiated from hESC cultures. In this review, we describe various methods tested to culture cells in the absence of MEF feeder layers and other advances in eliminating xenogeneic products from the culture system.

show abstract

“…In 2003, Park et al [20] first demonstrated that STO cells have the potential to support the establishment and maintenance of human ESC lines. Thereafter, STO cells have been widely used as feeder cells for human ESC and iPSC culture [24,25]. Proteome analyses have revealed that STO cells produce unique protein species, such as insulin-like growth factor binding protein 4 (IGFBP-4), pigment epithelium-derived factor (PEDF) and secreted protein acidic and rich in cysteine (SPARC, also known as osteonectin), which may be associated with differentiation and cell growth [26].…”

Section: Mouse-derived Feeder Cellsmentioning

confidence: 99%

Feeder Cell Sources and Feeder-Free Methods for Human iPS Cell Culture

Kamano

Wang

et al. 2015

Interface Oral Health Science 2014

View full text Add to dashboard Cite

Induced pluripotent stem cells (iPSCs) hold great promise for regenerative medicine and disease modeling. The original methods to grow human iPSCs utilized methods developed for human embryonic cells (ESCs), in which mitotically inactivated mouse-derived fibroblasts are mainly used as a "feeder" cell layer to maintain the undifferentiated status of pluripotent stem cells. However, these methods still require further consideration to facilitate cell expansion and to maintain the undifferentiated state of human iPSCs and/or ESCs for a longer period of time. In addition, the use of animal-derived feeders should be avoided for eventual clinical application of iPSC therapies. Therefore, human-derived feeder culture systems or feeder-free culture systems are currently being developed to prevent exposure to animal pathogens. In this review, existing mouse and human feeder culture systems for human ESCs and iPSCs are first introduced, and then previously reported feeder-free culture methods using extracellular matrixassociated products or synthetic biomaterials are outlined to discuss an appropriate culture system for clinical application of iPSCs.

show abstract

Establishment of human embryonic stem cell lines from frozen-thawed blastocysts using STO cell feeder layers

Abstract: This study indicates that establishment of hES cells from frozen-thawed blastocysts minimizes the ethical problem associated with the use of human embryos in research and that the STO cell feeder layer can be used for the culture of hES cells.

Cited by 74 publications

References 22 publications

A Survey of Parameters Involved in the Establishment of New Lines of Human Embryonic Stem Cells

A Survey of Parameters Involved in the Establishment of New Lines of Human Embryonic Stem Cells

Toward xeno-free culture of human embryonic stem cells

Feeder Cell Sources and Feeder-Free Methods for Human iPS Cell Culture

Contact Info

Product

Resources

About