Feeder Cell Sources and Feeder-Free Methods for Human iPS Cell Culture

Abstract

Induced pluripotent stem cells (iPSCs) hold great promise for regenerative medicine and disease modeling. The original methods to grow human iPSCs utilized methods developed for human embryonic cells (ESCs), in which mitotically inactivated mouse-derived fibroblasts are mainly used as a "feeder" cell layer to maintain the undifferentiated status of pluripotent stem cells. However, these methods still require further consideration to facilitate cell expansion and to maintain the undifferentiated state of human iPSCs and/or ESCs for a longer period of time. In addition, the use of animal-derived feeders should be avoided for eventual clinical application of iPSC therapies. Therefore, human-derived feeder culture systems or feeder-free culture systems are currently being developed to prevent exposure to animal pathogens. In this review, existing mouse and human feeder culture systems for human ESCs and iPSCs are first introduced, and then previously reported feeder-free culture methods using extracellular matrixassociated products or synthetic biomaterials are outlined to discuss an appropriate culture system for clinical application of iPSCs.

Generation of Human-Induced Pluripotent Stem Cells from Peripheral Blood Mononuclear Cells using Small-Molecule Compound VC6TFZ

“…This feeder cell produces stemness-supporting factors that prevent spontaneous differentiation. This feeder cell produced adhesion molecules and extracellular matrix that increases attachment of iPSCs which supports growth and survival [22], [23].…”

“…Vitronectin was an extracellular matrix protein that was rich in peptide arginine-glycine-aspartate (RGD) needed for integrin-mediated cell adhesion and growth through cellular pathway activation. Vitronectin also supports self-renewal and pluripotency from ESC [22]. In this study, we used rabbit adipose mesenchymal cells which mitosis-inactivated using 2 μg/mL mitomycin-C for 30 min.…”

Expression of SSEA4 and TRA1-60 as Marker of Induced Pluripotent Stem Cells by Small Molecule Compound VC6TFZ on Peripheral Blood Mononuclear Cell

“…One of the challenges in reprogramming PBMC was that these cells had non-adherent nature [12]. To overcome this problem, we coated the well walls using vitronectin and feeder cells that enable the PBMC attachment on the well.…”

“…This attachment was important to avoid cell lost during medium replacement. This feeder cell produced adhesion molecules and extracellular matrix that increases attachment of iPSCs which supports growth and survival [12,13].…”

Induced pluripotent stem cells (iPSCs) as a new source of bone in reconstructive surgery: A systematic review and meta-analysis of preclinical studies

“…Additionally, STO cells were developed due to the ease of maintenance than MEFs for preparation of feeder layers. Furthermore, SNL76/7 cells were used as they promoted long‐term maintenance of mouse ESCs by suppressing spontaneous differentiation (Yu et al, ). These feeder cell lines may be useful for laboratory experiments because they enable large‐scale expansion of iPSCs at low cost; however, they are associated with a high possibility of contamination.…”