Establishment of CRISPR-Cpf1-assisted gene editing tool and engineering of 4-hydroxyisoleucine biosynthesis in Corynebacterium glutamicum

Chen, Rui; Shi, Feng; Xiang, Youhe; Lai, Wenmei; Ji, Guohui

doi:10.1007/s11274-023-03705-1

Cited by 4 publications

(2 citation statements)

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“…While most examples of using CRISPR as counterselection are based on the use of recombinases (Figure 6), this system has also been employed to enhance the screening of second-crossover strains in suicidal plasmid systems. 29 The use of recombinases with a template can allow for the same wide variety of modifications detailed in the previous section (Figure 5), with the added advantage of this increase in efficiency. For instance, for E. coli strains, Cas9 selection can increase the recombination efficiency to 39%− 98% depending on the genomic modification.…”

Section: Acs Sustainablementioning

confidence: 99%

“…As a consequence, only mutated clones survive, thereby streamlining the process of clone selection while increasing the recombination efficiency. While most examples of using CRISPR as counterselection are based on the use of recombinases (Figure ), this system has also been employed to enhance the screening of second-crossover strains in suicidal plasmid systems . The use of recombinases with a template can allow for the same wide variety of modifications detailed in the previous section (Figure ), with the added advantage of this increase in efficiency.…”

Section: Introductionmentioning

confidence: 99%

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CRISPR Tools in Bacterial Whole-Cell Biocatalysis

Mulet,

Ripoll,

Betancor

2023

ACS Sustainable Chem. Eng.

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Biocatalysis has emerged as a promising alternative to conventional chemical processes for the production of a wide range of chemicals, providing a sustainable solution to the problem of limited resources due to an ever-increasing global population. This approach involves the use of biobased catalysts, such as whole microorganisms or enzymes, to perform chemical conversions. While whole-cell biocatalysts offer advantages over the use of free enzymes, limitations related to productivity and undesired compound production have been observed when using microorganisms. Offering high specificity, broad applicability, and increased efficiency over traditional genetic engineering methods, CRISPR-based technologies may be the quintessential tool for the fit-for-purpose design of efficient bacterial biocatalysts. In this work, we aim to demonstrate the potential of CRISPR-based technologies to enhance whole-cell bacterial biotransformations for a more sustainable obtention of industrially important products. We have included a comprehensive and in-depth analysis of the current state of the art, emphasizing challenges and opportunities for future research. Through a critical analysis of reported examples, we intend to highlight the opportunities and advantages offered by CRISPR-based technologies in the field of biocatalysis for more efficient, sustainable, and translational processes.

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Section: Acs Sustainablementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%