Establishment of an mKate2-Expressing Cell Line for Non-Invasive Real-Time Breast Cancer In Vivo Imaging

Vuletic, Ivan; Liu, Jinghao; Wu, Honglian; Ding, Yichen; Yu, Long-Chuan; Li, Changhui; Zhu, Daling; Ren, Qiushi; Sun, Hai; Li, Jun

doi:10.1007/s11307-015-0853-5

Cited by 7 publications

(6 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been reported that the expression of mKate-2 and luciferase have no effect on the biological behavior of parental cell. 18,19 Bioluminescence imaging (BLI) analysis confirmed that the light intensity, released from the VNP20009 strain expressing luciferase (“VNP20009-luc”), was proportional to bacterial counts (r 2 = 0.99). Additionally, VNP20009-luc displayed 0.36 ± 0.035 flux units per cfu in vitro ( Fig.…”

Section: Resultsmentioning

confidence: 94%

Suppression of pancreatic ductal adenocarcinoma growth by intratumoral delivery of attenuatedSalmonella typhimuriumusing a dual fluorescent live tracking system

Zhou

Zhao

Yang

et al. 2016

Cancer Biology & Therapy

View full text Add to dashboard Cite

Pancreatic ductal adenocarcinoma (PDAC) has the poorest prognosis among all malignancies and is resistant to almost all current therapies. Attenuated Salmonella typhimurium strain VNP20009 has been deployed as powerful anticancer agent in a variety of animal cancer models, and previous phase 1 clinical trials have proven its safety profiles. However, thus far, little is known about its effect on PDAC. Here, we established CFPAC-1 cell lines expressing an mKate2 protein and thus emitting far-red fluorescence in the subsequent xenograft implant. VNP20009 strain was further engineered to carry a luciferase cDNA, which catalyzes the light-emitting reaction to allow the observation of salmonella distribution and accumulation within tumor with live imaging. Using such VNP20009 strain and intratumoral delivery, we could reduce the growth of pancreatic cancer by inducing apoptosis and severe necrosis in a dosage dependent manner. Consistent with this finding, intratumoral delivery of VNP20009 also increase caspase-3 activity and the expression of Bax protein. In summary, we revealed that VNP20009 is a promising bacterial agent for the treatment of PDAC, and that we have established a dual fluorescent imaging system as a valuable tool for noninvasive live imaging of solid tumor and engineered bacterial drug.

show abstract

Section: Resultsmentioning

confidence: 94%

Suppression of pancreatic ductal adenocarcinoma growth by intratumoral delivery of attenuatedSalmonella typhimuriumusing a dual fluorescent live tracking system

Zhou

Zhao

Yang

et al. 2016

Cancer Biology & Therapy

View full text Add to dashboard Cite

show abstract

“…Bioluminescence imaging experiments were performed on a home-made imaging system [18]. Briefly, experimental mice were injected intraperitoneally with 150 mg/kg D-luciferin (15 mg/mL in sterile DPBS) at predetermined time points.…”

Section: Bioluminescent Imagingmentioning

confidence: 99%

Overcoming foreign-body reaction through nanotopography: Biocompatibility and immunoisolation properties of a nanofibrous membrane

et al. 2016

Self Cite

View full text Add to dashboard Cite

“…As a far-red fluorescent protein, higher brightness characterizes mKate2 and greater tissue penetration. It has, therefore, been widely used for labeling cells in vitro and real-time monitoring in vivo (15,16). In brief, the ADSCs were seeded into 24-well plates (20,000/well) and grown to 30-50% confluence.…”

Section: Labeling Of Adscsmentioning

confidence: 99%

Concentrated nanofat: a modified fat extraction promotes hair growth in mice via the stem cells and extracellular matrix components interaction

Zhang

et al. 2020

Ann Transl Med

View full text Add to dashboard Cite

Background: Fat graft transplantation seems a promising cell therapy for hair loss. However, impurities in lipoaspirate weaken the treatment effect. Here, we developed the lipoaspirate extraction method then investigate the effect and mechanism on hair growth-promoting in a mouse model. Methods: Fat graft was prepared into concentrated nanofat (CNF), decellularized CNF (DCNF), and adipose-derived stem cells (ADSCs). They were injected subcutaneously in the back of depilated mice to test the hair promoting effect. Conditioned media (CM) from the adipose extracts were applied to dermal papilla cells (DPCs) to evaluate the cell viability and the anagen related signal.Results: CNF and a high dose of ADSCs promoted hair growth and induced telogen-to-anagen transition in depilated mice. DCNF and a low dose of ADSCs did not show such effect; however, hair growth was promoted when they were used in combination. In vitro study showed the CNF-CM treated DPCs exhibited increased proliferation, migration, cell cycle progression, and elevated Wnt/β-catenin pathway protein levels compared with the other treatment groups.Conclusions: CNF has a better effect than ADSCs in hair promotion via activating the DPCs and anagen induction. In this nature complex of stem cells (SCs) and extracellular matrix (ECM), ECM serves a significant supplementary role and amplifies the power of ADSCs. These results supply a theoretical basis on the clinical application of CNF to treat hair loss.

show abstract

Establishment of an mKate2-Expressing Cell Line for Non-Invasive Real-Time Breast Cancer In Vivo Imaging

Abstract: In the present research, we have successfully built the MDA-MB-435s-mKate2 cell line that can be used as a suitable cell model for breast cancer therapy and anti-cancer drug evaluation by non-invasive fluorescence imaging in mice.

Cited by 7 publications

References 26 publications

Suppression of pancreatic ductal adenocarcinoma growth by intratumoral delivery of attenuatedSalmonella typhimuriumusing a dual fluorescent live tracking system

Suppression of pancreatic ductal adenocarcinoma growth by intratumoral delivery of attenuatedSalmonella typhimuriumusing a dual fluorescent live tracking system

Overcoming foreign-body reaction through nanotopography: Biocompatibility and immunoisolation properties of a nanofibrous membrane

Concentrated nanofat: a modified fat extraction promotes hair growth in mice via the stem cells and extracellular matrix components interaction

Contact Info

Product

Resources

About