Establishment of an immunofluorescence assay to detect IgM antibodies to Nipah virus using HeLa cells expressing recombinant nucleoprotein

Kaku, Yoshihiro; Park, Eun sil; Noguchi, Akira; Inoue, Satoshi; Lunt, Ross; Malbas, Fedelino F.; Demetria, Catalino S.; Neoh, Hui Min; Jamal, A. Rahman A.; Morikawa, Shigeru

doi:10.1016/j.jviromet.2019.03.009

Cited by 5 publications

(3 citation statements)

References 12 publications

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“…We determined the optimal conditions for thermal heating and UV irradiation for the inactivation of NiV in serum samples and growth medium and established a recommended protocol for NiV inactivation applicable in low-containment laboratories. Effective inactivation of NiV with the protocol would enable regional or local laboratories to manipulate clinical samples for serological assays such as neutralization tests as well as serological tests without infectious NiV, which we had developed before [ 33 – 35 ]. This will help in the rapid diagnosis of NiV disease in BSL-2 laboratories or regional bases for testing and in controlling large epidemics of the disease in future.…”

Section: Discussionmentioning

confidence: 99%

Effective inactivation of Nipah virus in serum samples for safe processing in low-containment laboratories

Watanabe

Fukushi

Harada

et al. 2020

Virol J

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Background Nipah virus (NiV) is an emerging zoonotic paramyxovirus that causes severe encephalitis and respiratory disease with a high mortality rate in humans. During large outbreaks of the viral disease, serological testing of serum samples could be a useful diagnostic tool, which could provide information on not only the diagnosis of NiV disease but also the history of an individual with previous exposure to the virus, thereby supporting disease control. Therefore, an efficient method for the inactivation of NiV in serum samples is required for serological diagnosis. Methods We determined the optimal conditions for the inactivation of NiV infectivity in human serum using heating and UV treatment. The inactivation method comprised UV irradiation with a cover of aluminum foil for 30 min and heating at 56 °C for 30 min. Results With an optimized protocol for virus inactivation, NiV infectivity in serum samples (containing 6.0 × 105 TCID50) was completely inactivated. Conclusions We developed a recommended protocol for the effective inactivation of NiV. This protocol would enable a regional or local laboratory to safely transport or process samples, including NiV, for serological testing in its biosafety level-2 facility.

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Section: Discussionmentioning

confidence: 99%

Effective inactivation of Nipah virus in serum samples for safe processing in low-containment laboratories

Watanabe

Fukushi

Harada

et al. 2020

Virol J

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show abstract

“…We determined the optimal conditions for thermal heating and UV irradiation for the inactivation of NiV in serum samples and growth medium and established a recommended protocol for NiV inactivation applicable in low-containment laboratories. Effective inactivation of NiV with the protocol would enable regional or local laboratories to manipulate clinical samples for serological assays such as neutralization tests as well as serological tests without infectious NiV, which we had developed before [31][32][33]. This will help in the rapid diagnosis of NiV disease in BSL-2 laboratories or regional bases for testing and in controlling large epidemics of the disease in future.…”

Section: Discussionmentioning

confidence: 99%

Effective inactivation of nipah virus in serum samples for safe processing in low-containment laboratories

Watanabe

Fukushi

Harada

et al. 2020

Preprint

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Background Nipah virus (NiV) is an emerging zoonotic paramyxovirus that causes severe encephalitis and respiratory disease with a high mortality rate in humans. During large outbreaks of the viral disease, serological testing of serum samples could be a useful diagnostic tool, which could provide information on not only the diagnosis of NiV disease but also the history of an individual with previous exposure to the virus, thereby supporting disease control. Therefore, an efficient method for the inactivation of NiV in serum samples is required for serological diagnosis. Methods We determined the optimal conditions for the inactivation of NiV infectivity in human serum using heating and UV treatment. The inactivation method comprised UV irradiation with a cover of aluminum foil for 30 min and heating at 56 °C for 30 min. Results With an optimized protocol for virus inactivation, NiV infectivity in serum samples (containing 6.4 × 105 TCID50) was completely inactivated. Conclusions We developed a recommended protocol for the effective inactivation of NiV. This protocol would enable a regional or local laboratory to safely transport or process samples, including NiV, for serological testing in its biosafety level-2 facility.

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“…Compared with SN test and ELISA, HI and IFA tests have the advantages of rapid, ease of setting‐up and cost‐effectiveness. In addition, IFA test can be used to differentiate among immunoglobulin isotypes, which is useful for diagnostic approach (Kaku et al., 2019; Niedrig et al., 2008). Several studies consistently showed that HI and IFA tests have good correlations with SN test for flaviviruses‐specific antibody detection (De Paula & Fonseca, 2004; Niedrig et al., 2008).…”

Section: Introductionmentioning

confidence: 99%

Evaluation and comparison of hemagglutination inhibition and indirect immunofluorescence tests for the detection of antibodies against duck Tembusu virus

Tunterak

Ninvilai

Prakairungnamthip

et al. 2022

Transbounding Emerging Dis

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Currently, duck Tembusu virus (DTMUV), an emerging avian pathogenic flavivirus, is widely spread and becomes endemic in duck populations in Asia, causing significant economic losses in the duck producing industry. To early detection and control of DTMUV, the well-validated diagnostic tests for efficient detection of DTMUV infection in ducks are needed. In this study, we validated and compared hemagglutination inhibition (HI) and indirect immunofluorescence (IFA) tests for identifying antibodies against DTMUV in duck serum samples. Our results demonstrated that HI and IFA tests can both be used to detect antibodies against DTMUV in duck serum samples with high sensitivity (100%), specificity (>87%) and overall agreement with the gold standard serum neutralization (SN) test (>90%). Additionally, DTMUV-specific antibody titres determined by HI and IFA tests correlated well with the neutralizing antibody titres obtained by SN test. No cross-reactivity against common duck viruses and other flaviviruses was observed in both tests. It is interesting to note that HI test had higher diagnostic specificity and exhibited a stronger positive correlation with SN test than IFA test. Evaluating the performance of HI and IFA tests with experimental and field serum samples revealed that both tests showed comparable performance with SN test in terms of antibody kinetic and detection rate. Collectively, these findings support the use of both tests, particularly HI test, as the alternative to SN test for measuring the antibody responses against DTMUV in ducks. These tests could be the suitable choices for DTMUV diagnosis, epidemiological study and vaccine efficacy evaluation.

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Establishment of an immunofluorescence assay to detect IgM antibodies to Nipah virus using HeLa cells expressing recombinant nucleoprotein

Cited by 5 publications

References 12 publications

Effective inactivation of Nipah virus in serum samples for safe processing in low-containment laboratories

Effective inactivation of Nipah virus in serum samples for safe processing in low-containment laboratories

Effective inactivation of nipah virus in serum samples for safe processing in low-containment laboratories

Evaluation and comparison of hemagglutination inhibition and indirect immunofluorescence tests for the detection of antibodies against duck Tembusu virus

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