Establishment of an Atlantic salmon kidney cell line with an inducible gene expression system

Collet, Bertrand; Lester, Katherine

doi:10.1016/j.jbiotec.2011.05.010

Cited by 9 publications

(3 citation statements)

References 10 publications

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“…During temperature optimization, it was found that high temperature could promote cell growth, and the growth rate showed an increasing trend within a certain temperature range. The result was consistent with those observed in the cell culture of other cold‐water fishes (Collet & Lester, 2011a, 2011b; Pham et al ., 2017; Rodriguez Saint‐Jean et al ., 2014). Although cell growth speeds up at higher temperatures, GPG cells grown at higher temperatures were more likely to shed and die under stresses, such as CO 2 cylinder replacement and temporary shutdown of laboratory power.…”

Section: Discussionsupporting

confidence: 92%

Establishment of a cell line derived from the gills ofGymnocypris przewalskii, an endemic Schizothoracine fish from Qinghai Lake of Tibet Plateau

Wei

Liang

Yue

et al. 2022

Journal of Fish Biology

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Gymnocypris przewalskii (Naked carp), a native teleost, plays an important role in maintaining the ecological balance of Lake Qinghai (altitude, 3.2 km), the largest saline lake in China. In this study, a new gill cell line from G. przewalskii was developed using the explant technique and named as GPG. This cell line was maintained in Dulbecco's Modified Eagle Medium (DMEM) (high glucose), supplemented with 15% fetal bovine serum (FBS), and was successfully subcultured up to 32 passages. Meanwhile, this cell line was also authenticated by sequencing the mitochondrial cytochrome C oxidase subunit I (COI) and 16S rRNA genes and by chromosome analysis. With the Cytomegalovirus (CMV) promoter, the GPG cell line could express green fluorescent protein (GFP) at about 5% transfection efficiency. MTT test showed that Clostridium botulinum toxin (BTX) was toxic to the cell line. After cryopreservation with 10% dimethyl sulfoxide (DMSO), this cell line could be successfully revived at an efficiency over 70%. This study revealed that the GPG cell line could be used as materials for physio‐chemical investigation of G. przewalskii and also provided a tool for gene function study and toxicological reaction in vitro.

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Section: Discussionsupporting

confidence: 92%

Establishment of a cell line derived from the gills ofGymnocypris przewalskii, an endemic Schizothoracine fish from Qinghai Lake of Tibet Plateau

Wei

Liang

Yue

et al. 2022

Journal of Fish Biology

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“…To address the issue regarding the loss of physiological function of transfected cells established via constitutive expression of Large T or Tert after long-term culture [16,17], a reversible cell line approach has recently received a great deal of interest for the establishment of multiple cell lines from other somatic tissues [18][19][20][21]. More functional phenotypes of the immortal cells might be recovered by turning off the expression of Large T or Tert using the Cre/LoxP-based inducible system [17].…”

Section: Introductionmentioning

confidence: 99%

Establishment of A Reversibly Inducible Porcine Granulosa Cell Line

Bai

Zhu

Mao

et al. 2020

Cells

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Granulosa cells (GCs) are the key components of ovarian follicles for regulating oocyte maturation. Previous established GC lines have allowed prolonged proliferation, but lost some physiological features owing to long-term immortalization. This study was to establish an induced immortal porcine GC line with reversible proliferation status by the tetracycline inducible (Tet-on) 3G system. Our conditional immortal porcine GCs (CIPGCs) line steadily propagated for at least six months and displayed primary GC morphology when cultured in the presence of 50 ng/mL doxycycline [Dox (+)]. Upon Dox withdrawal [Dox (–)], Large T-antigen expression, reflected by mCherry fluorescence, gradually became undetectable within 48 h, accompanied by less proliferation and size increase. The levels of estradiol and progesterone, and the expression of genes associated with steroid production, such as CYP11A1 (cytochrome P450 family 11), 3β-HSD (3β-hydroxysteroid dehydrogenase), StAR (steroidogenic acute regulatory protein), and CYP19A1 (cytochrome P450 family 19 subfamily a member 1), were all significantly higher in the Dox (–) group than Dox (+) group. The CIPGCs could switch into a proliferative state upon Dox induction. Interestingly, the expression of StAR and CYP19A1 in the CIPGCs (–Dox) was significantly increased by adding porcine follicular fluid (PFF) to mimic an ovary follicle environment. Moreover, PFF priming the CIPGCs in Dox (–) group resulted in similar estradiol production as that of primary GC, and enabled this cell line to respond to gonadotrophins in estradiol production. Collectively, we have established an inducible immortal porcine GC line, which offers a unique and valuable model for future research on the regulation of ovarian functions.

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“…So far, a good few cell lines from freshwater and marine fish species have been reported from a broad range of species and tissues (Ou-Yang et al, 2010;Wang et al, 2010;Babu et al, 2011;Collet and Lester, 2011;Lakra et al, 2011 andYan et al, 2011). The investigators in the tropics regions are lacking cell lines of their choice, and which may be the reason for the limited number of studies on viruses from warm water fish species (Sunil et al, 2001 andkhamiss andHashem, 2012).…”

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confidence: 99%

Establishment of a cell culture system and characterization of the primary cultures from different organs of the Nile tilapia Oreochromis niloticus

Hashem¹,

Meshhal²,

Zahran³

et al. 2020

Egypt. J. of Aquatic Biolo. and Fish.

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Cell culture from fish is an excellent tool for many advanced applications. The cell lines derived from a particular fish species are more appropriate for studying the viruses reported from that species and studying virus-host interactions (Crane and Hyatt, 2011 and Pandey, 2013). It is an excellent alternative to obtain good quality chromosome preparations in cytogenetic studies of small and large species. With the establishment of a cell bank available at any time and in case of a repetition of cytogenetic methodologies, it is not necessary to go back to the field for new individuals (Paim et al., 2018). Fish cell lines are appropriate for in vitro assays, since they are believed to retain fish-specific traits in their metabolism of chemicals (Schenel et al., 2015). Cell cultures lack in vivo complexities such as bioaccumulation and depuration. Finally, experiments with cell ARTICLE INFO

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Establishment of an Atlantic salmon kidney cell line with an inducible gene expression system

Cited by 9 publications

References 10 publications

Establishment of a cell line derived from the gills ofGymnocypris przewalskii, an endemic Schizothoracine fish from Qinghai Lake of Tibet Plateau

Establishment of a cell line derived from the gills ofGymnocypris przewalskii, an endemic Schizothoracine fish from Qinghai Lake of Tibet Plateau

Establishment of A Reversibly Inducible Porcine Granulosa Cell Line

Establishment of a cell culture system and characterization of the primary cultures from different organs of the Nile tilapia Oreochromis niloticus

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