Establishment of a Pure Vascular Endothelial Cell Line from Human Placenta

Jinga, V.; Gafencu, Anca Violeta; Antohe, Felicia; Constantinescu, E; Heltianu, Constantina; Raicu, Monica; Manolescu, I; Hunziker, Walter; Simionescu, Maya

doi:10.1053/plac.1999.0492

Cited by 40 publications

(21 citation statements)

References 28 publications

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“…As IgG is found in the stroma [2], we assume that it diffuses through the stroma and thus gains access to the abluminal surface of the fetal endothelium. Based on hFcRn expression in fetal endothelial cells and on transport studies in isolated, polarized cultures of vascular endothelial cells derived from the human placenta [28,29], we suggest that transendothelial transport is also mediated by hFcRn by similar mechanisms as described above for placental STB (Fig. 3).…”

Section: Mechanism Of Transplacental Igg Transportmentioning

confidence: 55%

HFcRn-mediated transplacental immunoglobulin G transport: Protection of and threat to the human fetus and newborn

Ellinger

Fuchs

2012

Wien Med Wochenschr

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In human newborns, endogenous levels of plasma immunoglobulin G (IgG) begin to rise slowly after birth following exposure to the environment. For immunoprotection during fetal and early neonatal life, maternal IgG is provided by transplacental transport. While cellular immunoprotective IgG effects are mainly triggered by FcγRI, -RII and -RIII, transplacental IgG transfer is mediated by the MHC class I-like neonatal Fc-receptor, hFcRn. This compact review explains the mechanism of hFcRn-mediated IgG transcytosis across the placental barrier - syncytiotrophoblast and fetal endothelial cells. Restrictions of this IgG transport are summarized. These include IgG subclass discrimination and limited IgG transport before the third trimester that can cause insufficient protection from infections of preterm (≤ 35 th week) delivered babies. As hFcRn does not discriminate beneficial from hazardous IgGs, maternal auto- and alloimmune as well as therapeutic antibodies can reach the fetus. The consequences including severe diseases of the newborn are summarized in this article.

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Section: Mechanism Of Transplacental Igg Transportmentioning

confidence: 55%

HFcRn-mediated transplacental immunoglobulin G transport: Protection of and threat to the human fetus and newborn

Ellinger

Fuchs

2012

Wien Med Wochenschr

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“…The cells had a typical cobblestone morphology exhibiting a slightly elongated polygonal shape and displayed the characteristic biochemical markers for an endothelial cell line such as von Willebrand factor antigen, angiotensin converting enzyme, and uptake of acetylated low-density lipoproteins, as previously described [9]. The transendothelial electrical resistance of HPEC monolayer varied between 22 and 52 ⍀cm 2 , in the range of reported resistance for cultured bovine aortic endothelial cells [17].…”

Section: Characterization Of Cultured Placental Endothelial Cellsmentioning

confidence: 67%

“…HPEC were isolated from term placenta villi, cultured in MCDB with 15% fetal calf serum, and grown to confluence either on culture plates or on semipermeable filters (Transwell-COL) as previously described [9,10].…”

Section: Cellsmentioning

confidence: 99%

“…These data raised the question on the identity of the receptor or receptors that mediate the transfer of maternal IgG across the endothelial barrier. The isolation of human placental endothelial cells (HPEC) [9] and the high expression of FcRn on these cells [10] enabled the study of the transport of human IgG across cultured HPEC [10,11]. We have found that regulation of IgG level by HPEC results from interplay between salvaging, recycling, and transcytosis.…”

Section: Introductionmentioning

confidence: 99%

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Neonatal Fc receptors discriminates and monitors the pathway of native and modified immunoglobulin G in placental endothelial cells

Rădulescu¹,

Antohe²,

Jinga³

et al. 2004

Human Immunology

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“…The accessory proteins of the ZO provide structural support and bind to integral membrane proteins of the tight junctions, further linking membrane proteins to the actin cytoskeleton [4][5][6]. A key feature to measure the integrity of the blood-brain barrier is that it exhibits high transendothelial electrical resistance, TEER (approximately 2,000 ohm/cm 2 ) [7], while endothelial cells from the human placenta exhibit less than 50 ohm/cm 2 [8]. The high selectivity ensures that even small molecules such as dyes and antibiotics are prevented from entry into the CNS, while non-brain endothelium is more permissive.…”

Section: Model Of the Blood-brain Barrier In Vitromentioning

confidence: 99%

Non-Mammalian Models to Study Infections of the Central Nervous System and to Screen Potential Therapeutic Agents

Khan

Siddiqui²

2010

TOPROCJ

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Abstract:Infections of the central nervous system present the most significant challenge in our attempt to combat infectious diseases. Part of our inability to counter these diseases is attributed to an incomplete understanding of (i) how infectious agents cross the blood-brain barrier, a key step in the development of CNS infections, and (ii) the inability of drugs to traverse the blood-brain barrier to gain entry into the brain to kill parasites. Thus, there is an urgent need to expedite both drug discovery and drug delivery programmes. Here, we describe the use of in vitro, ex vivo, and in vivo models of the blood-brain barrier to examine the physical barriers, cellular mechanisms and molecular elements participating from both sides of the parasite-host interactions. In addition, these models provide useful screening tools to give rough indications of the potential of new antimicrobial agents with respect to efficacy, optimal routes of administration, and toxicity.Keywords: Blood-brain barrier, in vivo models, in vitro models, locusts, human brain microvascular endothelial cells.

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Establishment of a Pure Vascular Endothelial Cell Line from Human Placenta

Cited by 40 publications

References 28 publications

HFcRn-mediated transplacental immunoglobulin G transport: Protection of and threat to the human fetus and newborn

HFcRn-mediated transplacental immunoglobulin G transport: Protection of and threat to the human fetus and newborn

Neonatal Fc receptors discriminates and monitors the pathway of native and modified immunoglobulin G in placental endothelial cells

Non-Mammalian Models to Study Infections of the Central Nervous System and to Screen Potential Therapeutic Agents

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