Establishment of a method to determine the magnetic particles in mouse tissues

Wu, Yifan; Zhang, Wuxu; Wang, Yuxia; Li, Qian; Gao, Guo; Dong, Na; Hu, Hengyao; Wang, Kan; Wu, Junhua; Gao, Zhongcai; Cui, Daxiang

doi:10.1186/1556-276x-7-665

Cited by 7 publications

(2 citation statements)

References 24 publications

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“…The concentration of iron in the nanosuspension was measured by the thiocyanate method [ 35 , 36 ]. In brief, 0.1 mL of the sample was diluted with 0.30 mL concentrated HNO 3 , kept for 10 min at 80 °C, and then cooled to room temperature.…”

Section: Methodsmentioning

confidence: 99%

Magnetic Relaxation Switching Assay Using IFNα-2b-Conjugated Superparamagnetic Nanoparticles for Anti-Interferon Antibody Detection

et al. 2023

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Type I interferons, particularly IFNα-2b, play essential roles in eliciting adaptive and innate immune responses, being implicated in the pathogenesis of various diseases, including cancer, and autoimmune and infectious diseases. Therefore, the development of a highly sensitive platform for analysis of either IFNα-2b or anti-IFNα-2b antibodies is of high importance to improve the diagnosis of various pathologies associated with the IFNα-2b disbalance. For evaluation of the anti-IFNα-2b antibody level, we have synthesized superparamagnetic iron oxide nanoparticles (SPIONs) coupled with the recombinant human IFNα-2b protein (SPIONs@IFNα-2b). Employing a magnetic relaxation switching assay (MRSw)-based nanosensor, we detected picomolar concentrations (0.36 pg/mL) of anti-INFα-2b antibodies. The high sensitivity of the real-time antibodies’ detection was ensured by the specificity of immune responses and the maintenance of resonance conditions for water spins by choosing a high-frequency filling of short radio-frequency pulses of the generator. The formation of a complex of the SPIONs@IFNα-2b nanoparticles with the anti-INFα-2b antibodies led to a cascade process of the formation of nanoparticle clusters, which was further enhanced by exposure to a strong (7.1 T) homogenous magnetic field. Obtained magnetic conjugates exhibited high negative MR contrast-enhancing properties (as shown by NMR studies) that were also preserved when particles were administered in vivo. Thus, we observed a 1.2-fold decrease of the T2 relaxation time in the liver following administration of magnetic conjugates as compared to the control. In conclusion, the developed MRSw assay based on SPIONs@IFNα-2b nanoparticles represents an alternative immunological probe for the estimation of anti-IFNα-2b antibodies that could be further employed in clinical studies.

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Section: Methodsmentioning

confidence: 99%

Magnetic Relaxation Switching Assay Using IFNα-2b-Conjugated Superparamagnetic Nanoparticles for Anti-Interferon Antibody Detection

et al. 2023

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“…The mice were sacrificed at the desired time, and then the hydrogels were excised from the skin and weighed. The residual Au–Fe 3 O 4 nanoparticles and siPLK-1 that remained in the hydrogels were measured using the potassium thiocyanide colorimetric analysis and the RiboGreen assay, respectively. , The Au–Fe 3 O 4 nanoparticles targeted to the tumor were also accessed, simultaneously. The excised tumor tissues were freeze-dried, weighed, dissolved in nitric acid, mineralized, and diluted in distilled water.…”

Section: Methodsmentioning

confidence: 99%

Injectable and Quadruple-Functional Hydrogel as an Alternative to Intravenous Delivery for Enhanced Tumor Targeting

Zhang

Kim

Song

2019

ACS Appl. Mater. Interfaces

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Intravenous (IV) route is the most commonly used drugdelivery approach. However, the targeting efficiency to tumor through IV delivery is usually less than 10%. To address this limitation, we report a new systemic delivery method utilizing injectable and quadruple-functional hydrogels to improve targeting efficiency through passive, active, and magnetic targeting, and hydrogel-controlled sustained release. The hydrogels consist of a folate/polyethylenimine-conjugated poly(organophosphazene) polymer, which encapsulates small interfering RNA (siRNA) and Au−Fe 3 O 4 nanoparticles to form a nanocapsule (NC) structure by a simple mixing. The hydrogels are localized as a long-term "drug-release depot" after a single subcutaneous injection and sol−gel phase transition. NCs released from the hydrogels enter the circulatory systems and then target the tumor through enhanced permeability and retention/folate/magnetism triple-targeting, over the course of circulation, itself prolonged by the controlled release. In vivo experiments show that 12% of NCs are successfully delivered to the tumor, which is a considerable improvement compared to most results through IV delivery. The sustained targeting of gold to tumor enables two cycles of photothermal therapy, resulting in an enhanced silencing effect of siRNA and considerable reduction of tumor volume, which we are unable to achieve via simple intravenous injection.

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A novel colorimetric technique for estimating iron in magnetosomes of magnetotactic bacteria based on linear regression

Rajalakshmi¹,

Anjukam²,

Ramesh³

et al. 2022

Arch Microbiol

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Establishment of a method to determine the magnetic particles in mouse tissues

Cited by 7 publications

References 24 publications

Magnetic Relaxation Switching Assay Using IFNα-2b-Conjugated Superparamagnetic Nanoparticles for Anti-Interferon Antibody Detection

Magnetic Relaxation Switching Assay Using IFNα-2b-Conjugated Superparamagnetic Nanoparticles for Anti-Interferon Antibody Detection

Injectable and Quadruple-Functional Hydrogel as an Alternative to Intravenous Delivery for Enhanced Tumor Targeting

A novel colorimetric technique for estimating iron in magnetosomes of magnetotactic bacteria based on linear regression

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