Establishment of a leukocyte cell line derived from peritoneal macrophages of fish, Labeo rohita (Hamilton, 1822)

Awasthi, Abhishek Kumar; Rathore, Gaurav; Sood, Neeraj; Khan, M. Firoze; Lakra, W. S.

doi:10.1007/s10616-013-9660-5

Cited by 15 publications

(13 citation statements)

References 44 publications

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“…The current research aimed to assess the phagocytic and bactericidal activity of peritoneal macrophages in the uptake of WT E. ictaluri and two LAV candidate strains. Our conclusions agree with those of multiple studies that document strong phagocytic capability and bactericidal activity of peritoneal macrophages against intracellular pathogens ( Esteban and Meseguer, 1997 ; António et al, 1998 ; Do Vale et al, 2002 ; Awasthi et al, 2015 ). We documented enhanced vaccine strains phagocytic capacity and effective bacterial killing ability of catfish peritoneal macrophages in vitro .…”

Section: Discussionsupporting

confidence: 92%

“…Winter flounder ( Pleuronectes americanus ) peritoneal macrophages were capable of engulfing formalin-fixed bacteria ( Y. ruckeri and Bacillus cereus ) after a short exposure ( Bodammer and Robohm, 1996 ). The phagocytic and microbicidal activity of peritoneal macrophages were described in rohu ( Labeo rohita ) and walking catfish ( Clarias batrachus ) ( Mamnur Rashid et al, 2002 ; Awasthi et al, 2015 ). Shoemaker et al (1997) evaluated the role of peritoneal macrophages in immunity to ESC after infection with live E. ictaluri .…”

Section: Introductionmentioning

confidence: 99%

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Phagocytic and Bactericidal Properties of Channel Catfish Peritoneal Macrophages Exposed to Edwardsiella ictaluri Live Attenuated Vaccine and Wild-Type Strains

et al. 2018

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Edwardsiella ictaluri (E. ictaluri), a Gram-negative, intracellular, facultative bacterium, is the causative agent of enteric septicemia of catfish (ESC), which is one of the most significant diseases of farmed channel catfish. Macrophages have a critical role in major defense mechanisms against bacterial infections by migrating to the site of infection, engulfing and killing pathogens, and priming adaptive immune responses. Vaccination of catfish with E. ictaluri live attenuated vaccine (LAV) strains increased the efficiency of phagocytosis and bacterial killing in catfish peritoneal macrophages compared in vitro with macrophages from non-vaccinated fish. Recently, our group developed several protective LAV strains from E. ictaluri. However, their effects on the antigen uptake and bacterial killing in catfish macrophages have not been evaluated. In this study, we assessed the phagocytic and bactericidal activity of peritoneal macrophages in the uptake of E. ictaluri wild-type (WT) and two LAV strains. We found that phagocytosis of LAV strains was significantly higher compared to their WT counterpart in peritoneal macrophages. Moreover, the uptake of E. ictaluri opsonized with sera from vaccinated catfish was more efficient than when opsonized with sera from sham-vaccinated fish. Notably, catfish macrophages did not lose their phagocytic properties at 4°C, as described previously in mammalian and zebrafish models. Also, opsonization of E. ictaluri with inactivated sera from vaccinated and sham-vaccinated catfish decreased significantly phagocytic uptake of bacteria at 32°C, and virtually suppressed endocytosis at 4°C, suggesting the important role of complement-dependent mechanisms in catfish macrophage phagocytosis. In conclusion, our data on enhanced phagocytic capacity and effective killing ability in macrophages of vaccine strains suggested the LAVs’ advantage if processed and presented in the form of peptides to specific lymphocytes of an adaptive immune system and emphasize the importance of macrophage-mediated immunity against ESC. Furthermore, we showed the role of complement-dependent mechanisms in the phagocytic uptakes of E. ictaluri in catfish peritoneal macrophages at 4 and 32°C. Finally, LAV vaccine-induced bacterial phagocytosis and killing properties of peritoneal macrophages emphasized the importance of the innate immune responses in ESC.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Phagocytic and Bactericidal Properties of Channel Catfish Peritoneal Macrophages Exposed to Edwardsiella ictaluri Live Attenuated Vaccine and Wild-Type Strains

et al. 2018

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“…phagocytosis is the basic body defense and plays a role in limitting and breaking the foreign cells. The role of phagocytosis is mediated by monocytes, neutrophils, and macrophages as presented from the value of phagocytic activity (Awasthi et al, 2013). Phagocyte cells will kil the bacteria by producing reactive oxygens during the respiratory burst process (Uribe et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Growth performance and immune response of catfish Clarias sp. given probiotics Bacillus megaterium PTB 1.4 and Pediococcus pentosaceus E2211

Hamka

Meryandini

Widanarni

2020

Jurnal Akuakultur Indonesia

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ABSTRACT Motile aeromonad septicaemia (MAS) in catfish can be done by improving the immune responses through probiotic administration. Co-administering probiotics producing digestive enzymes are expected to have an impact on fish growth. This study was aimed to evaluate the effectivity of probiotics B. megaterium PTB 1.4 and P. pentosaceus E2211 to improve the growth performance and immune response of catfish infected by Aeromonas hydrophila. Catfish with the initial body weight of 7.36 ± 0.21 g were reared in a pond. This study was conducted with five treatments, i. e. K- (without probiotic addition and A. hydrophila injection), K+ (no probiotic addition with A. hydrophila injection), Bm (B. megaterium PTB 1.4 addition and A. hydrophila injection), Pp (P. pentosaceus E2211 addition and A. hydrophila injection), and Bm+Pp (B. megaterium PTB 1.4 addition and P. pentosaceus E2211 and A. hydrophila injection). The study results showed that Bm, Pp, and Bm+Pp treatments were able to improve the growth performance of catfish including digestive enzyme activity, daily growth rate, feed conversion ratio, and final biomass with the best results was Bm+Pp treatment. The immune response of catfish before and after the challenge test showed better survival rate, higher total leukocytes, phagocytic activity, respiratory burst, and lysozyme activity on Bm, Pp, and Bm+Pp treatment (P<0.05) than the control treatment. In conclusion, the combination of probiotics B. megaterium PTB 1.4 and P. pentosaceus E2211 in feed synergistically improved the growth performance and immune response on catfish against A. hydrophila infection. Keywords: Aeromonas hydrophila, catfish, growth performance, immune response, probiotics ABSTRAK Motile aeromonad septicaemia (MAS) pada ikan lele dapat dikendalikan melalui respons imun inang dengan pemberian probiotik. Pemberian bersama probiotik penghasil enzim pencernaan diharapkan dapat berdampak pada pertumbuhan ikan. Penelitian ini bertujuan mengevaluasi efektivitas pemberian probiotik B. megaterium PTB 1.4 dan P. pentosaceus E2211 terhadap kinerja pertumbuhan dan respons imun ikan lele terhadap infeksi A. hydrophila. Ikan lele dengan bobot 7.36 ± 0.21 g dipelihara pada kolam beton yang diberi waring. Penelitian ini menggunakan rancangan acak lengkap dengan lima perlakuan, yaitu: K- (tanpa probiotik dan tanpa diinjeksi A. hydrophila), K+ (tanpa probiotik dan diinjeksi A. hydrophila), Bm (diberi B. megaterium PTB 1.4 dan diinjeksi A. hydrophila), Pp (diberi P. pentosaceus E2211 dan diinjeksi A. hydrophila), dan Bm+Pp (diberi B. megaterium PTB 1.4 dan P. pentosaceus E2211 dan diinjeksi A. hydrophila). Hasil penelitian menunjukkan perlakuan Bm, Pp, dan Bm+Pp mampu meningkatkan pertumbuhan ikan lele. Aktivitas enzim pencernaan, laju pertumbuhan harian, rasio konversi pakan, dan biomassa panen dengan hasil terbaik yaitu perlakuan Bm+Pp. Respons imun ikan lele dengan kombinasi probiotik B. megaterium PTB 1.4 dan P. pentosaceus E2211 mampu meningkatkan sintasan, total leukosit, aktivitas fagositosis, respiratory burst, dan aktivitas lisozim, baik pada sebelum dan setelah uji tantang. Pemberian kombinasi probiotik B. megaterium PTB 1.4 dan P. pentosaceus E2211 pada pakan mampu bekerja sinergis dalam meningkatkan kinerja pertumbuhan dan respons imun ikan lele terhadap infeksi A. hydrophila. Kata kunci: A. hydrophila, ikan lele, kinerja pertumbuhan, probiotik, respons imun

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“…The number of cells was counted using a cell counting chamber (Wuxi Devan Scientific Ltd., Co., Wuxi, China). According to reports, after incubating the peritoneal cells for 1 h, >98% of adherent cells in culture dishes were identified as macrophages due to the observation of Fc receptor-mediated phagocytosis and the Giemsa staining performed (28,29).…”

Section: Methodsmentioning

confidence: 99%

Acetic acid alleviates the inflammatory response and liver injury in septic mice by increasing the expression of TRIM40

et al. 2019

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Sepsis remains a significant health care issue in clinical practice due to its high mortality rate and healthcare cost, despite extensive efforts to better understand the pathophysiology of sepsis. The systemic inflammatory response often leads to severe liver injury, even acute liver dysfunction and failure. Acetic acid, as a type of chemical compound, has been reported to be an emerging drug for improving metabolic syndrome and inhibiting inflammation in rats and human. To verify the effects of acetic acid in protecting the liver and reducing the inflammatory response, a septic mouse model was established by cecal ligation and puncture (CLP), and then the CLP-model mice were treated with acetic acid or PBS. Following the treatment, it was determined that, in CLP-model mice, acetic acid could alleviate the inflammatory response by decreasing the expression of cytokines including interleukin-6 and tumor necrosis factor-α. Additionally, acetic acid also alleviated the liver injury, and the levels of alanine aminotransaminase, aspartate aminotransferase, Toll-like receptor (TLR)4 and nuclear factor-κB (NF-κB) were decreased. The expression of tripartite motif-containing protein (TRIM)40 was also upregulated significantly. Therefore, the authors of the current study hypothesized that acetic acid could decrease the inflammatory response by increasing the expression of TRIM40 and TRIM40 may regulate the activity of the TLR4 signaling pathway. To further illustrate the interaction between TRIM40 and the TLR4 signaling pathway, the authors collected macrophages from the peritoneal cavity by intraperitoneally administering mice with 5 ml ice-cold normal saline. Following the collection, peritoneal macrophages were treated with acetic acid, TRIM40 small interfering RNA or PBS. It was demonstrated that acetic acid upregulated the expression of TRIM40. When TRIM40 was silenced, the protective effect of acetic acid would be reversed as well. The results suggested that TRIM40 could act on and downregulate the activity of the TLR4 signaling pathway. TRIM40 is possibly the major target for acetic acid, which may function as a protective factor in septic mice.

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Establishment of a leukocyte cell line derived from peritoneal macrophages of fish, Labeo rohita (Hamilton, 1822)

Cited by 15 publications

References 44 publications

Phagocytic and Bactericidal Properties of Channel Catfish Peritoneal Macrophages Exposed to Edwardsiella ictaluri Live Attenuated Vaccine and Wild-Type Strains

Phagocytic and Bactericidal Properties of Channel Catfish Peritoneal Macrophages Exposed to Edwardsiella ictaluri Live Attenuated Vaccine and Wild-Type Strains

Growth performance and immune response of catfish Clarias sp. given probiotics Bacillus megaterium PTB 1.4 and Pediococcus pentosaceus E2211

Acetic acid alleviates the inflammatory response and liver injury in septic mice by increasing the expression of TRIM40

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