Establishment of a Cell Line (CNUH-HNSCC-1) Derived from an Advanced Laryngeal Squamous Cell Carcinoma

Lee, Dong Hoon; Lee, Joon Kyoo

doi:10.4068/cmj.2011.47.2.85

Cited by 3 publications

(10 citation statements)

References 15 publications

(15 reference statements)

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“…HNSCC has several specific markers, such as p53, EGFR, erbB‐2, and Ki‐67. Among these, we confirmed expression of the tumor suppressor genes p16 and p53, and the oncogenes ras, myc, EGFR, erbB‐2, and cyclin D1 . Genetic alterations, such as deletion of tumor suppressor genes and amplification of oncogenes, have been identified .…”

Section: Discussionmentioning

confidence: 52%

“…Our results support a similar previous claim that notch is a possible HNSCC cancer marker. P53 is commonly altered in tobacco‐related cancers, and the EGFR protein is altered in HNSCC and other malignant cancers; thus, forming a well‐known therapeutic target …”

Section: Discussionmentioning

confidence: 99%

“…Among these, we confirmed expression of the tumor suppressor genes p16 and p53, and the oncogenes ras, myc, EGFR, erbB-2, and cyclin D1. 15,21,22 Genetic alterations, such as deletion of tumor suppressor genes and amplification of oncogenes, have been identified. 21 Notch, an HNSCC-associated protein, is an important tumor suppressor protein.…”

Section: Discussionmentioning

confidence: 99%

“…Permanent HNSCC tumors cell lines are important tools for research into the molecular, biochemical, and genetic properties of head and neck cancer . Likewise, our newly established cancer cell line can be used as a powerful tool for various head and neck cancer research, new therapies, and preclinical studies on cancer progression …”

Section: Introductionmentioning

confidence: 99%

“…14 Likewise, our newly established cancer cell line can be used as a powerful tool for various head and neck cancer research, new therapies, and preclinical studies on cancer progression. 15 In this article, we describe the development and characterization of a new radio-sensitive HPV-negative HNSCC cell line.…”

Section: Introductionmentioning

confidence: 99%

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Establishment of a novel human papillomavirus–negative and radiosensitive head and neck squamous cell carcinoma cell line

et al. 2015

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Section: Discussionmentioning

confidence: 52%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Establishment of a novel human papillomavirus–negative and radiosensitive head and neck squamous cell carcinoma cell line

et al. 2015

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An Effective Primary Head and Neck Squamous Cell Carcinoma In Vitro Model

Oppel

Shao

Schürmann

et al. 2019

Cells

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Head and neck squamous cell carcinoma is a highly malignant disease and research is needed to find new therapeutic approaches. Faithful experimental models are required for this purpose. Here, we describe the specific cell culture conditions enabling the efficient establishment of primary cell culture models. Whereas a classical 10% serum-containing medium resulted in the growth of fibroblast-like cells that outcompeted epithelial cells, we found that the use of specific culture conditions enabled the growth of epithelial tumor cells from HPV+ and HPV− head and neck cancer tissue applicable for research. EpCAM and high Thy-1 positivity on the cell surface were mutually exclusive and distinguished epithelial and fibroblast-like subpopulations in all primary cultures examined and thus can be used to monitor stromal contamination and epithelial cell content. Interestingly, cells of an individual patient developed tumor spheroids in suspension without the use of ultra-low attachment plates, whereas all other samples exclusively formed adherent cell layers. Spheroid cells were highly positive for ALDH1A1 and hence displayed a phenotype reminiscent of tumor stem cells. Altogether, we present a system to establish valuable primary cell culture models from head and neck cancer tissue at high efficiency that might be applicable in other tumor entities as well.

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Precision Medicine in Head and Neck Cancers: Genomic and Preclinical Approaches

Miserocchi

Spadazzi

Calpona

et al. 2022

JPM

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Head and neck cancers (HNCs) represent the sixth most widespread malignancy worldwide. Surgery, radiotherapy, chemotherapeutic and immunotherapeutic drugs represent the main clinical approaches for HNC patients. Moreover, HNCs are characterised by an elevated mutational load; however, specific genetic mutations or biomarkers have not yet been found. In this scenario, personalised medicine is showing its efficacy. To study the reliability and the effects of personalised treatments, preclinical research can take advantage of next-generation sequencing and innovative technologies that have been developed to obtain genomic and multi-omic profiles to drive personalised treatments. The crosstalk between malignant and healthy components, as well as interactions with extracellular matrices, are important features which are responsible for treatment failure. Preclinical research has constantly implemented in vitro and in vivo models to mimic the natural tumour microenvironment. Among them, 3D systems have been developed to reproduce the tumour mass architecture, such as biomimetic scaffolds and organoids. In addition, in vivo models have been changed over the last decades to overcome problems such as animal management complexity and time-consuming experiments. In this review, we will explore the new approaches aimed to improve preclinical tools to study and apply precision medicine as a therapeutic option for patients affected by HNCs.

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Establishment of a Cell Line (CNUH-HNSCC-1) Derived from an Advanced Laryngeal Squamous Cell Carcinoma

Cited by 3 publications

References 15 publications

Establishment of a novel human papillomavirus–negative and radiosensitive head and neck squamous cell carcinoma cell line

Establishment of a novel human papillomavirus–negative and radiosensitive head and neck squamous cell carcinoma cell line

An Effective Primary Head and Neck Squamous Cell Carcinoma In Vitro Model

Precision Medicine in Head and Neck Cancers: Genomic and Preclinical Approaches

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