Establishment of a novel human papillomavirus–negative and radiosensitive head and neck squamous cell carcinoma cell line

Kim, KyungMin; Park, Eun-Ji; Yeo, Jiyoung; Joo, Young‐Hoon; Cho, Kwang-Jae; Kim, Min‐Sik

doi:10.1002/hed.24037

Cited by 2 publications

(1 citation statement)

References 27 publications

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“…Nearly 50% of OSCC patients, with the exception of those with distant metastases, have clinical or pathological lymph node metastases, and metastases to the lungs and bones have also been reported 2,3) . Several tongue-derived squamous cell carcinoma cell lines have been described, and there is an association between tongue cancer and excessive alcohol consumption, smoking history, and human papillomavirus infection [4][5][6][7][8] . Commonly used human tongue squamous cell carcinoma cell lines include the highly invasive SAS cells and less invasive clones as well as the highly metastatic LMF3, LMF4, and LMF5 cell lines derived from human oral tongue squamous cell carcinoma.…”

Section: Introductionmentioning

confidence: 99%

Establishment and Characterization of the Human Tongue Squamous Cell Carcinoma Cell Line NOKT-1

Sakuma

Takahashi

Kii

et al. 2021

J. Hard Tissue Biology.

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The squamous cell carcinoma cell line NOKT-1 was successfully established from the right tongue of a 74-yearold Japanese man. Pathological diagnosis of the original tumor was moderately differentiated squamous cell carcinoma. NOKT-1 cells were transplanted subcutaneously into nude mice and xenograft was formed. In addition, the NOKT-1-XG cell line was established from the transplanted tumor of NOKT-1 cells. NOKT-1 cells and NOKT-1-XG cells were epithelial neoplastic and pleomorphic cells, which were similar. Immunocytochemistry revealed that NOKT-1 and NOKT-1-XG cells were CK17 and human mitochondria positive. To authenticate the NOKT-1 cell line and NOKT-1-XG cell line, we examined cross-contamination with other cell lines using short tandem repeat analysis, the results of which showed that NOKT-1 and NOKT-1-XG are new cell lines. Four of the 16 loci, corresponding to 25%, were different between these two cell lines, which indicates that the NOKT-1 genome was altered by transplantation. Moreover, in AM, NOKT-1 did not have a Y chromosome, whereas NOKT-1-XG had. Despite the genetic differences, a collagen gel droplet-embedded culture drug susceptibility test demonstrated that NOKT-1 cells derived from the original tumor and the NOKT-1-XG cell line had the same sensitivity. This cell line could be very useful for the development of immunotherapy and chemotherapy regimens and research on cancer etiology.

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Section: Introductionmentioning

confidence: 99%

Establishment and Characterization of the Human Tongue Squamous Cell Carcinoma Cell Line NOKT-1

Sakuma

Takahashi

Kii

et al. 2021

J. Hard Tissue Biology.

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Establishment and characterization of a squamous cell carcinoma cell line, designated hZK-1, derived from a metastatic lymph node tumor of the tongue

et al. 2017

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The hZK-1 cell line was successfully established from the metastatic foci of a lymph node of an 82-year-old Japanese woman with squamous cell carcinoma of the tongue. The pathological diagnosis of the tumor was moderately to well-differentiated squamous cell carcinoma. The hZK-1 cells were angular in shape, and had neoplastic and pleomorphic features. Adjacent hZK-1 cells were joined by desmosomes and well-developed microvilli, and many free ribosomes were observed in the cytoplasm. The doubling time of the hZK-1 cells was approximately 36, 33, and 29 h at the 10th, 20th, and 30th passages, respectively. The cell line was shown to be triploid, with a chromosomal distribution of 75-80. Immunocytochemical staining of the hZK-1 cells revealed cytokeratin (CK) 17-, Ki67-, and p53-positive staining, and negative staining for CK13. The hZK-1 cells were negative for human papillomavirus (HPV)-16 or-18 infection. Grafting was not successful when the hZK-1 cells were transplanted into the subcutis of SCID mice. The hZK-1 cells (2 × 10 cells/3 ml of growth medium) secreted vascular endothelial growth factor (VEGF) that reached a concentration of 2.6 ng/ml media after 3 days of culture. Hypoxia enhanced cellular HIF-1α expression and VEGF secretion in hZK-1 cells. The HIF-1α inhibitor YC-1 partially inhibited hypoxia-induced VEGF secretion in ZK-1 cells. The reverse transcription-polymerase chain reaction (RT-PCR) results revealed that the expression of CK17, Ki67, and p53 was elevated in the hZK-1 cells. hZK-1 cells were not sensitive to CDDP, TXT, 5-FU, or a mixture of these three anti-tumor agents.

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Establishment of a novel human papillomavirus–negative and radiosensitive head and neck squamous cell carcinoma cell line

Abstract: CMCSCC-1, a novel oral tongue SCC cell line, was established. It will help in the elucidation of the molecular pathogenesis of HPV-negative radiosensitive tumors. © 2015 Wiley Periodicals, Inc. Head Neck 38: E542-E551, 2015.

Cited by 2 publications

References 27 publications

Establishment and Characterization of the Human Tongue Squamous Cell Carcinoma Cell Line NOKT-1

Establishment and Characterization of the Human Tongue Squamous Cell Carcinoma Cell Line NOKT-1

Establishment and characterization of a squamous cell carcinoma cell line, designated hZK-1, derived from a metastatic lymph node tumor of the tongue

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