Establishment and Optimization Growth of Shoot Buds-Derived Callus and Suspension Cell Cultures of <i>Kaempferia parviflora</i>

Abstract

Callus and suspension cells culture of Kaempferia parviflora was successfully established. Meristematic shoots can be used for utilization of plant cell biosynthetic capabilities for obtaining useful products from valuable medicinal plant to meet out the pharmaceutical demand and also for studying the metabolism.

Influence of N6-Benzyladenine and Sucrose on In Vitro Direct Regeneration and Microrhizome Induction of Kaempferia parviflora Wall. Ex Baker, An Important Ethnomedicinal Herb of Asia

“…It has also been reported in Zingiber officinale that after 3 months of culture supplemented with 10% sucrose plantlets exhibited symptoms of vitrification and bud decay ( Archana et al 2013 ). There are two known reports for microrhizome induction of K. parviflora, one using liquid culture system and another using high sucrose concentrations ( Zuraida et al 2014 ; Kafindra et al 2015 ). Zuraida et al (2014) reported that liquid MS medium supplemented with 4.44 μM BA and 1 NAA and 6% sucrose was most effective for microrhizome formation with 25.6 ± 3.4 g/flask produced in 3 months of culture.…”

“…There are two known reports for microrhizome induction of K. parviflora, one using liquid culture system and another using high sucrose concentrations ( Zuraida et al 2014 ; Kafindra et al 2015 ). Zuraida et al (2014) reported that liquid MS medium supplemented with 4.44 μM BA and 1 NAA and 6% sucrose was most effective for microrhizome formation with 25.6 ± 3.4 g/flask produced in 3 months of culture. It was also reported that higher sucrose concentration to 9%–12% retarded plantlets and caused plantlet death.…”

“…In vitro studies in K. parviflora are limited with only few known reports ( Dheeranupattana et al 2003 ; Mongkolchaipak et al . 2006 ; Prathanturarug et al 2007 ; Alveno 2012 ; Zuraida et al 2014 ) whereas report on microrhizome production is minimal. Microrhizome production is a technique of inducing rhizomes under in vitro conditions.…”

“…Therefore, to overcome obstacles such as year-round availability and increase the amount of high quality planting material, micropropagation or in vitro mass multiplication in K. parviflora was investigated. In vitro studies in K. parviflora are limited with only few known reports (Dheeranupattana et al 2003;Mongkolchaipak et al 2006;Prathanturarug et al 2007;Alveno 2012;Zuraida et al 2014) whereas report on microrhizome production is minimal. Microrhizome production is a technique of inducing rhizomes under in vitro conditions.…”

Establishment of a Rapid Micropropagation System for Kaempferia parviflora Wall. Ex Baker: Phytochemical Analysis of Leaf Extracts and Evaluation of Biological Activities

“…In vitro plant regeneration [ 20 , 21 ], microrhizome formation [ 20 , 25 ], and cell suspension-based culturing [ 26 ] of K. parviflora have been reported. Axillary shoot multiplication has been achieved using terminal shoot buds [ 21 ] or rhizomatous buds [ 20 ].…”