Development of suspension culture technology and hormone effects on anthocyanin biosynthesis for red Cyclocarya paliurus cells

Liu, Yuan; Qi, Liang; Tang, Daobang; Chen, Yuqin; Zang, Jianwei; Zhao, Wenjia; Chen, Jiguang; Yin, Zhong‐Ping

doi:10.1007/s11240-021-02215-y

Cited by 4 publications

(1 citation statement)

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“…At present, polyphenols are mainly obtained from plant materials such as leaves, stems, flowers and fruits by extraction and purification ( Cui et al, 2014 ). There are lots of plants that can be used in plant cell culture to produce polyphenols, but plants have a long growth cycle and a vulnerability to the natural environment and pests ( Liu et al, 2022 ). It is also costly to get polyphenols of high purity because of a considerable variety of components in the differentiated plant organs like leaves and flowers ( Silva et al, 2015 ), which restricts the availability of natural plant polyphenols ( Dea et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Investigating the induction of polyphenol biosynthesis in the cultured Cycolocarya paliurus cells and the stimulatory mechanism of co-induction with 5-aminolevulinic acid and salicylic acid

Ling

Wang²,

Pan³

et al. 2023

Front. Bioeng. Biotechnol.

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Background: Plant cell culture technology is a potential way to produce polyphenols, however, this way is still trapped in the dilemma of low content and yield. Elicitation is regarded as one of the most effective ways to improve the output of the secondary metabolites, and therefore has attracted extensive attention.Methods: Five elicitors including 5-aminolevulinic acid (5-ALA), salicylic acid (SA), methyl jasmonate (MeJA), sodium nitroprusside (SNP) and Rhizopus Oryzae Elicitor (ROE) were used to improve the content and yield of polyphenols in the cultured Cyclocarya paliurus (C. paliurus) cells, and a co-induction technology of 5-ALA and SA was developed as a result. Meanwhile, the integrated analysis of transcriptome and metabolome was adopted to interpret the stimulation mechanism of co-induction with 5-ALA and SA.Results: Under the co-induction of 50 μM 5-ALA and SA, the content and yield of total polyphenols of the cultured cells reached 8.0 mg/g and 147.12 mg/L, respectively. The yields of cyanidin-3-O-galactoside, procyanidin B1 and catechin reached 28.83, 4.33 and 2.88 times that of the control group, respectively. It was found that expressions of TFs such as CpERF105, CpMYB10 and CpWRKY28 increased significantly, while CpMYB44 and CpTGA2 decreased. These great changes might further make the expression of CpF3′H (flavonoid 3′-monooxygenase), CpFLS (flavonol synthase), CpLAR (leucoanthocyanidin reductase), CpANS (anthocyanidin synthase) and Cp4CL (4-coumarate coenzyme A ligase) increase while CpANR (anthocyanidin reductase) and CpF3′5′H (flavonoid 3′, 5′-hydroxylase) reduce, ultimately enhancing the polyphenols accumulationConclusion: The co-induction of 5-ALA and SA can significantly promote polyphenol biosynthesis in the cultured C. paliurus cells by regulating the expression of key transcription factors and structural genes associated with polyphenol synthesis, and thus has a promising application.

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Section: Introductionmentioning

confidence: 99%