Establishment and characterization of two primary breast cancer cell lines from young Indian breast cancer patients: mutation analysis

Abstract

Two novel triple negative breast cancer cell lines, NIPBC-1 and NIPBC-2 were successfully established from primary tumors of two young breast cancer patients aged 39 and 38 years respectively, diagnosed as infiltrating duct carcinoma of breast. Characterization of these cell lines showed luminal origin with expression of epithelial specific antigen and cytokeratin 18 and presence of microfilaments and secretary vesicles, microvilli, tight junctions and desmosomes on ultra-structural analysis. Both the cell lines showed anchorage independent growth and invasion of matrigel coated membranes. Karyotype analysis showed aneuploidy, deletions and multiple rearrangements in chromosomes 7, 9, X and 11 and isochromosomes 17q in both the cell lines. P53 mutational analysis revealed no mutation in the coding region in both the cell lines; however NIPBC-2 cell line showed presence of heterozygous C/G polymorphism, g.417 C > G (NM_000546.5) resulting in Arg/Pro allele at codon 72 of exon 4. Screening for mutations in BRCA1&2 genes revealed presence of three heterozygous polymorphisms in exon 11 of BRCA1 and 2 polymorphisms in exons 11, and14 of BRCA2 gene in both the cell lines. Both the cell lines showed presence of CD 44+/24-breast cancer stem cells and capability of producing mammosphere on culture. The two triple negative breast cancer cell lines established from early onset breast tumors can serve as novel invitro models to study mechanisms underlying breast tumorigenesis in younger age group patients and also identification of new therapeutic modalities targeting cancer stem cells.

BRCA1 Gene's EXON 11 and Breast Carcinoma: A Mutational Hot Spot for Familial Patients and Prone to Metastases in Northern India

“…A recent study of Pandrangi S L from New Delhi, India established Two novel triple negative breast cancer cell lines, NIPBC-1 and NIPBC-2 from primary tumors of two young breast cancer patients aged 39 and 38 years respectively, diagnosed as infiltrating duct carcinoma of breast. Screening for mutations in BRCA gene revealed presence of three heterozygous polymorphisms in exon 11 of BRCA1 gene in both the cell lines [24]. The allocation of BRCA1 mutations recognized among Indian high-risk patients is similar to what has been observed in other populations.…”

Establishment and genomic characterization of primary salivary duct carcinoma cell line

“…Developing thoroughly characterized cell lines, from this rare cancer type is central to accelerating research and development. [15–19] We report the establishment and characterization of the first cell line derived from primary untreated salivary duct carcinoma. Importantly, the cell line shared lineage and molecular characteristics and both the full-length AR and the AR-V7 isoform with the parent tumor.…”

Establishment and Characterization of a New Triple Negative Breast Cancer Cell Line from an Iranian Breast Cancer Tissue

“…While cancer cell lines maintain many features of the tumor tissue (Neve et al, 2006;Vargo-Gogola and Rosen, 2007;Van Staveren et al, 2009), there are some difficulties with using them. For instance, the majority of them have been isolated from a metastatic tumor of older patients and many of these cell lines have been in culture for decades (Ku et al, 2013;Pandrangi et al, 2014). In addition, the genetic diversity of tumor cells may cause unpredictable tumor behavior and treatment failure.…”