Establishment and genomic characterization of primary salivary duct carcinoma cell line

Li, Jie; Mitani, Yoshitsugu; Rao, Pulivarthi H.; Perlaky, László; Liu, Bin; Weber, Randal S.; El‐Naggar, Adel K.

doi:10.1016/j.oraloncology.2017.04.007

Cited by 7 publications

(10 citation statements)

References 35 publications

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“…To date, there are several previous reports on the establishment of cell lines for SGC [27][28][29][30][31][32][33][34][35] . In terms of SDC cell line, MDA-SDC-04 is the only SDC cell line established using a 2-dimensional culture reported until now 31 , while Li et al reported that the line requires an immortalization process and loses chromosomal aberrations by long-term passaging without any tumor-forming potential in xenografts. Thus, it is di cult to establish SGC cell lines using traditional 2-dimensional culture that reproduces the original tumor characteristics.…”

Section: Discussionmentioning

confidence: 99%

Novel Preclinical Human Salivary Gland Cancer Models Established Using Organoid Culture And Patient-Derived Xenografting

Aizawa¹,

Takada²,

Aoyama³

et al. 2021

Preprint

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Salivary gland carcinoma (SGC) has poor prognosis depending on the histological subtype. However, due to the scarcity of preclinical experimental models, its pathogenesis remains largely unknown, hampering the development of new treatment modalities for patients with these malignancies. Here, we first report the establishment of a large collection of human SGC experimental models for multiple histological subtypes using patient-derived xenograft (PDX) and organoid culture techniques with our previously optimized method. Additionally, we successfully generated organoids by culturing established PDXs (PDX-derived organoids). Through passaging, each pre-clinical model was confirmed to maintain the pathological characteristics of the original tumor and the genetic traits of corresponding histological subtypes. Finally, we confirmed that these organoids were available for pharmacologic studies. Thus, our comprehensive models of SGC could be a powerful resource for the development of novel therapeutic agents and investigating the pathogenesis of these malignancies.

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Section: Discussionmentioning

confidence: 99%

Novel Preclinical Human Salivary Gland Cancer Models Established Using Organoid Culture And Patient-Derived Xenografting

Aizawa¹,

Takada²,

Aoyama³

et al. 2021

Preprint

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“…SDC cell lines female derived RET981 (7,41), male derived MDA-SDC-04 (SDC-04; ref. 42), and MAC (43) were used. Both RET981 and SDC-04 cells express full length AR.…”

Section: Sdc and Prostate Cancer Cell Linesmentioning

confidence: 99%

Reciprocal and Autonomous Glucocorticoid and Androgen Receptor Activation in Salivary Duct Carcinoma

Mitani

Lin

Pytynia

et al. 2020

Clinical Cancer Research

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Purpose: To determine the expression of glucocorticoid receptor (GR) and androgen receptor (AR) in salivary duct carcinoma (SDC) and to analyze the role of these proteins in the development and management of this disease entity.Experimental Design: We performed a phenotypic assessment of GR and AR localization and expression, and determined their association with clinicopathologic factors in 67 primary SDCs. In vitro functional and response analysis of SDC cell lines was also performed.Results: Of the 67 primary tumors, 12 (18%) overexpressed GR protein, 30 (45%) had constitutive expression, and 25 (37%) had complete loss of expression. Reciprocal GR and AR expression was found in 32 (48%) tumors, concurrent constitutive GR and AR expression in 23 (34%), and simultaneous loss of both receptors and high GR with AR expressions were found in 12 (18%). GR overexpression was significantly associated with worse clinical outcomes. In vitro ligand-independent AR activation was observed in both male-and female-derived cell lines. GR antagonist treatment resulted in decreased cell proliferation and survival in GRoverexpressing cells, irrespective of AR status. Reciprocal GR-and AR-knockdown experiments revealed an independent interaction.Conclusions: Our study, for the first time, demonstrates differential GR and AR expressions, autonomous GR and AR activation, and ligand-independent AR expression and activation in SDC cells. The findings provide critical information on the roles of GR and AR steroid receptors in SDC tumorigenesis and development of biomarkers to guide targeted steroid receptor therapy trials in patients with these tumors.

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“…For an epithelial cell line, such observations are based on examination of the cellular morphology and expression pattern of the epithelial marker cytokeratin [ 9 ]. In addition, any changes in chromosomes that may have been induced by the immortalization protocol are screened by karyotype analysis [ 10 ] and/or the presence of gene mutations from the progenitor cell using whole-exome sequencing [ 11 ]. Actually, ovarian cancer has been known to originate from the ovarian surface epithelium (OSE) since the mid-90s to early 2000s [ 12 – 15 ].…”

Section: Introductionmentioning

confidence: 99%

Establishment of five immortalized human ovarian surface epithelial cell lines via SV40 T antigen or HPV E6/E7 expression

Shin¹,

Yang²,

Lee³

et al. 2018

PLoS ONE

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BackgroundHuman ovarian surface epithelial (HOSE) cells are a critical cell source for ovarian cancer research; however, they are difficult to obtain and maintain under standard laboratory conditions in large quantities. The aim of this study was to generate immortalized HOSE (IHOSE) cells with maintained properties to the original cell source, thereby guaranteeing a sufficiently large cell quantity for ovarian cancer research.MethodsHOSE cells isolated from four non-cancer patients and five IHOSE cell lines were established by induction of HPV-E6/E7 expression or SV40 large T antigen using a lenti-viral system. Each of IHOSE cells was confirmed to be distinct by STR profiling. RNA-sequencing was used to compare gene expression profiles in HOSE, IHOSE and ovarian cancer cells.ResultsRNA-sequencing results revealed a stronger linear correlation in gene expression between IHOSE and HOSE cells (R2 = 0.9288) than between IHOSE or HOSE cells and ovarian cancer cells (R2 = 0.8562 and R2 = 0.7982, respectively). The gene expression pattern of 319 differentially expressed genes revealed minimal differences between HOSE and IHOSE cells, while a strong difference between ovarian cancer cells and HOSE or IHOSE cells was observed. Furthermore, the five IHOSE cell lines displayed morphological characteristics typical of epithelial cells but showed a lower level of EpCAM, CD133 and E-cadherin, as cancer stem marker, than ovarian cancer cells. Moreover, unlike cancer cells, IHOSE cells could not form colonies in the anchorage-independent soft agar growth assay.ConclusionThese findings demonstrate that five newly established IHOSE cell lines have characteristics of progenitor HOSE cells while exhibiting continuous growth, and thus, should be highly useful as control cells for ovarian cancer research.

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Establishment and genomic characterization of primary salivary duct carcinoma cell line

Cited by 7 publications

References 35 publications

Novel Preclinical Human Salivary Gland Cancer Models Established Using Organoid Culture And Patient-Derived Xenografting

Novel Preclinical Human Salivary Gland Cancer Models Established Using Organoid Culture And Patient-Derived Xenografting

Reciprocal and Autonomous Glucocorticoid and Androgen Receptor Activation in Salivary Duct Carcinoma

Establishment of five immortalized human ovarian surface epithelial cell lines via SV40 T antigen or HPV E6/E7 expression

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