Establishment and Characterization of the MSKR Inbred Strain Originated from Japanese Wild Mice(Mus musculus molossinus).

Abstract

. A new inbred strain, MSKR, originated from Japanese wild mice was established in April, 1998. The MSKR mice were 60% of the C57BL/6N inbred mice in the 60-day body weight. Tail length/head-body length and hind-foot length/head-body length of the MSKR mice were significantly smaller than those of the C57BL/6N mice (0.896 vs 1.061, 0.189 vs 0.204), but ear length/head-body length of the MSKR mice was significantly larger than that of the C57BL/6N mice (0.143 vs 0.137). The age of the first parturition and size of the first litter were 63.20 ± 2.71 days and 6.20 ± 0.37, respectively, at the 20th and 22nd inbreeding generations. Genetic characterization of the MSKR strain was performed using 34 microsatellite markers, 29 biochemical markers, 9 immunogenetic markers, 3 coat color markers, and mitochondrial DNA RFLP-haplotypes. The result indicated that this newly established inbred strain has some different gene constitution from already known molossinus and common laboratory strains.-KEY WORDS: inbred strain, mouse, Mus musculus molossinus.

The absence of mitochondrial DNA diversity among common laboratory inbred mouse strains

“…Inbred mouse strains can be classified into two categories, classical and wild-derived, and the latter consists of several dozens of strains derived from wild mice that were trapped at different times and places and from different populations (Ideraabdullah et al, 2004). Some of these inbred strains were derived from Mus species and Mus musculus subspecies and also from their intersubspecific hybrids (Bonhomme and Guenet, 1996;Wada et al, 2000).…”

Identification of compatibility between ooplasmic factor and sperm gene in the intersubspecific crosses involving DDK and PWK mice strains

“…The limited gene variation is undesirable in inbred mice that will be used in various research fields. One of the methods to solve this problem is to introduce new genes from a wild genetic pool to a laboratory genetic pool (Wada et al, 2000). Various remarkable achievements make many wild-derived mouse strains useful for DDK syndrome and many other researches in the future, such as MOM, CASP, PWD/Ph, PREA, PREC and PWK (Gregorová and Forejt, 2000;Zhao et al, 2002;Storchová et al, 2004;Ideraabdullah et al, 2007).…”